Characterization of Wild Blueberry Polyphenols Bioavailability and Kinetic Profile Over 24-hour Period

This study is a randomized, single blinded, 2-arm, within-subjects, placebo-controlled design
utilizing a multiple sampling, repeated measures paradigm to characterize the bioavailability
and kinetic profile of wild blueberry polyphenols.

A planned sample size of 12 will be enrolled into the study. This study will require one
initial screening visit, one pre-study visit, and 2 study visits. This study will take
approximately 3-4 weeks per subject to complete.

The initial screening visit will provide subject with the informed consent document and
determine subject eligibility through anthropometric measurements, vital signs, fasting blood
glucose test (finger prick), and completion of a survey relate to general eating, health, and
exercise habits.

If willing and eligible to participate, a 3-day food record (2 weekdays and 1 weekend day)
will be instructed at the Screening Visit and collected at the Pre-study visit to assess
subjects' baseline dietary intake pattern. Subjects will be instructed to follow a strictly
limited polyphenolic diet for 7 days prior to the study and throughout their participation
time, while maintaining their usual diet pattern and physical activity, with counseling by
staff investigator and/or Registered Dietitian. A dinner meal will be provided the day before
the study visit to control the second meal effect from the food and beverage intake of the
night before the study visit.

Subject will arrive at the center in a fasted state for at least 10 hours, well hydrated and
rested. Each study visit will require blood draws throughout the visit. After evaluation of
subject's health status (via anthropometric, vital sign and blood glucose measurements and
in-person interview), a registered nurse will place a catheter in subject's arm for the
purpose of multiple blood sample collections and take the initial blood draw in the fasting
state. Subjects will be randomized to receive a placebo or wild blueberry beverage treatment
based on randomized treatment sequences at 2 study visits along with 3 standard meals
(breakfast, lunch and dinner). The sequences of receiving the beverage treatments at each
visit will be randomly assigned to one of two of following sequences: placebo-wild blueberry
or wild blueberry-placebo.

Each study visit will involve with blood samples collection at time points 0 (fasting), 0.25,
0.5, 1, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 24 hour (h) for assessment of change in plasma
metabolites of wild blueberry polyphenols and influence of metabolic and inflammation
markers. Also, urine samples will be collected prior to the study treatment as a baseline and
all voids over 6 time periods, 0 to 2 h, 2 to 4 h, 4 to 6 h, 6 to 8 h, 8 to 10h and 10 to 24
h during each study visit to assess wild blueberry polyphenolic metabolites.

Inclusion Criteria:

- 20-45 years of age

- Body Mass Index (BMI) range from 25 to 33 kg/m2

- Nonsmoker

- No clinical evidence of cardiovascular, metabolic, respiratory, renal,
gastrointestinal or hepatic disease

- Not taking any medications that would interfere with outcomes of the study, i.e. lipid
lowering medications, anti-inflammatory drugs, dietary supplements, etc…

- Able to provide informed consent

- Able to comply and perform the procedures requested by the protocol

- Weight stable: not gained or lost weight +/- 5 kg in previous 3 months

Exclusion Criteria:

- Men and women who smoke

- Past smokers: abstinence for minimum 2 years

- Men and women with known or suspected food intolerance, allergies or hypersensitivity

- Men and women known to have/diagnosed with diabetes mellitus

- Men and women who have fasting blood glucose concentrations > 110mg/dL

- Men and women who have uncontrolled blood pressure >120 mmHg/80 mmHg

- Men and women with documented vascular disease, e.g., heart failure, myocardial
infarction, stroke, angina, related surgeries, etc...

- Men and women with cancer other than non-melanoma skin cancer in previous 5 years

- Women who are known to be pregnant or who are intending to become pregnant over the
course of the study

- Women who are lactating

- Taking medication or dietary supplements that may interfere with the outcomes of the
study; e.g., antioxidant supplement, anti-inflammation, lipid lowering medication,
blood pressure lowering medication, etc... Subjects may choose to go off dietary
supplements (requires 30 days washout); e.g., fish oil, probiotics, etc...

- Men and women who have donated blood within 3 months of the Screening Visit and blood
donors/participants for whom participation in this study will result in having donated
more than 1500 milliliters of blood in the previous 12 months.

- Men and women who are vegans

- Substance (alcohol or drug) abuse within the last 2 years

- Excessive coffee and tea consumers (> 4 cups/d) and berry consumers (>2 cups fresh/day

- Men and women who do excessive exercise regularly or athlete

- Unstable weight: gained or lost weight +/- 5 kg in previous 3 months

- Women who are taking unstable dose and brand of hormonal contraceptives and/or stable
dose and brand less than 6 months.