The Role of Vitamin D in Female Reproductive Tract Immunity

This outpatient open label study will assess the impact of 8 weeks of daily versus weekly
VitD and calcium supplementation on measures of cervicovaginal (CV) innate immune function,
including antimicrobial and proinflammatory gene expression, Toll-like receptor
(TLR)-mediated responses, immunohistochemical (IHC) analysis of immune cell markers, and
microbiota in premenopausal women with insufficient serum VitD levels at baseline.

Women will be seen in 3 study visits and will be contacted by one scheduled follow-up
telephone call after their final study visit. Volunteers will be consented at Visit 1 and
undergo procedures to confirm they are eligible to continue in the study.

Once it has been confirmed that the participant meets all of the inclusion criteria and none
of the exclusion criteria, baseline blood and genital samples will be taken in the luteal
phase at Visit 2. The participant will then be given their first dose of VitD 4000 IU daily
or 50,000 IU weekly in capsule form, which they will then administer for 8 weeks.
Participants will also administer 1000 mg of calcium daily. Participants will return upon
completion of supplementation for repeat of blood and genital samples (Visit 3).

Inclusion Criteria:

- Serum 25(OH)D ≤ 25ng/mL (62 nmol/L)

- In good health, as evidenced by history and procedures at screening visits without
any clinically significant systemic disease.

- Not at risk of an STI, meaning: In a monogamous heterosexual or same sex relationship
with a partner who is not known to be HIV positive and has no known risks for STIs,
or Sexually abstinent

- Willing and able to comply with study procedures

- 21 to 50 years of age

- Not at risk for pregnancy, meaning: Protected from pregnancy due to surgical
sterilization of participant and or her sexual partner, Consistent condom use, In a
same sex relationship or, Abstinent from sexual activity and planning to remain
abstinent for the duration of the study

- Regular menstrual cycles (every 21 - 35 days) for the past two cycles, per
participant report

- Willing to use sunscreen of SPF15 or higher when exposed to the sun for at least 15
minutes, and to avoid use of non-study Vitamin D, calcium, and multivitamin
supplementation.

Exclusion Criteria:

- A clinically significant history of an abnormal Pap test (by written report) in the
past year that has not been evaluated and/or treated, if indicated, according to
standard guidelines

- Surgery or biopsy of the vagina or cervix within 14 days

- Current STI or lower genital tract infection including HIV-1, Chlamydia trachomatis
(CT), Trichomonas vaginalis (TV), Neisseria Gonorrhoeae (NG), Hepatitis B, yeast
vaginitis or bacterial vaginosis (BV) (by Nugent score of 7 - 10); this does not
include asymptomatic HSV or human papilloma virus (HPV)

- Current presence of vulvar, anal and/or vaginal genital warts

- Current tobacco use of any amount

- Body Mass Index (BMI) of ≥ 35 kg/m2

- History of sensitivity/allergy to any component of the study product, topical
anesthetic, or allergy to silver nitrate and/or Monsel's solution

- Known bleeding disorder that could lead to prolonged or continuous bleeding with
biopsy, or taking anticoagulant drugs

- Grade 2 or higher laboratory abnormality, per the August 2009 update of the DAIDS
Table for Grading the Severity of Adverse Events

- Systemic use in the last two weeks or anticipated use during the study of any of the
following: corticosteroids, non-steroidal anti-inflammatory drugs (NSAIDs),
antibiotics, anticoagulants or other drugs known to prolong bleeding and/or clotting,
antifungals, antivirals (e.g., acyclovir or valacyclovir) or antiretrovirals (e.g.,
Viread). Note: Participants should avoid NSAIDS except for treatment of dysmenorrhea
during menses. Participants may use acetaminophen on an as-needed but not daily basis
during the study.

- Known risk factors for hypercalcemia (e.g. malignancy or granulomatous disease) or
malabsorption syndromes (e.g. celiac disease, radiation enteritis, active
inflammatory bowel disease).

- Other conditions that, in the opinion of the investigator, would constitute
contraindications to participation in the study or would compromise the ability to
comply with study protocols, such as any major chronic illness including but not
limited to cancer, serious autoimmune disease, metabolic disorders or a major
psychiatric disorder (e.g., schizophrenia)

- Current participation in any other drug or device study, or any study which, in the
opinion of the investigator, would jeopardize interpretation of results of this study

- Use of depot medroxyprogesterone acetate (DMPA) in last six months

- Use of any other hormonal contraceptive method (oral, transdermal, transvaginal,
implant, or intrauterine device) without 2 subsequent, normal menses since stopping
hormonal contraceptives

- Current use of copper IUD

- Currently pregnant or pregnancy within the past 3 months

- Currently breastfeeding or having breastfed an infant in the last two months