Disease Risk Reduction and Omega-3 Rich Rainbow Trout (Fish for Health)

Consumption of fish high in long chain omega-3 fatty acids (LCn3) is associated with
reduction of cardiovascular disease (CVD) risk. CVD is a major cause of death in the United
States. The current obesity epidemic contributes significantly to elevated CVD risk by
increasing inflammation and platelet dysfunction. Prevention of CVD is a public health goal
and comprises several avenues of action, of which inclusion of LCn3-rich fish in the diet may
be one of the most effective.

Obese participants will participate in a double blind, randomized, cross-over designed trial
in which they will consume twice-weekly a prepared meal containing diploid (2N) farmed
rainbow trout, triploid (3N) farmed rainbow trout containing higher levels of LCn3, or
tilapia, a low LCn3 fish.

Inclusion Criteria:

- BMI of 30 - 39.9 kg/m2

Exclusion Criteria:

- Are allergic to fish

- Use tobacco products or nicotine in any form including snuff, pills, and patches, or
e-cigarettes in the past 6 months

- Have established cardiovascular, pulmonary, and/or a metabolic disease such as
diabetes

- Have uncontrolled high blood pressure

- Have alcohol, anabolic steroids, or other substance abuse issues

- Consume more than 3 alcoholic drinks/week

- Have cancer

- Are pregnant or nursing

- Take non-steroidal anti-inflammatory drugs such as aspirin, ibuprofen or Aleve, lipid
modifying drugs such as statins, take medications for blood glucose such as insulin or
metformin