Muscle Insulin Resistance in Aging (Mirage)

Study Objectives:

1. To determine the effects of diet-induced weight loss with and without the addition of
exercise on mitochondrial biogenesis and energetic capacity, cellular redox state and
insulin resistance.

2. To determine the effects of diet-induced weight loss with and without the addition of
exercise on intramyocellular lipid profiles.

3. To determine the effects of diet-induced weight loss with and without exercise on
skeletal muscle proteins mediating a program of autophagy and either loss or maintenance
of muscle mass.

Inclusion Criteria:

- 65-80 years of age

- Stable weight (No Gain/Loss of >10 lbs in 6 months)

- Sedentary (≤ 1 continuous exercise/week)

- Non-smoker

- BMI ≥ 30 kg/m2

- Resting Blood Pressure ≤ 150 millimeters of mercury systolic and ≤ 95 millimeters of
mercury diastolic

- Note from Primary care physician/Cardiologist for exercise clearance if positive
stress test symptoms were observed from exercise test

- Must be willing to washout for 14 days from all diabetes medication and independent in
self blood glucose monitoring during the washout periods (those with diabetes only)

Exclusion Criteria:

- Clinically significant cardiovascular disease including history of myocardial
infarction, within the past year

- Peripheral Vascular Disease

- Hepatic, renal, muscular/neuromuscular, or active hematologic/oncologic disease

- Clinically diminished pulse

- Presence of bruits in lower extremities

- Previous history of pulmonary emboli

- Peripheral Neuropathy