Comparing the Effect of Whey and Soy Protein on the Absorption of Pomegranate Polyphenols in the Human Body
| Status: | Completed |
|---|---|
| Conditions: | Healthy Studies |
| Therapuetic Areas: | Other |
| Healthy: | No |
| Age Range: | 20 - 45 |
| Updated: | 2/7/2015 |
| Start Date: | August 2014 |
| End Date: | August 2015 |
| Contact: | Gail D Thames, BA |
| Email: | gthames@mednet.ucla.edu |
| Phone: | 310-825-0453 |
Dietary Protein in Pomegranate Ellagitannin Bioavailability
This study is comparing the quantity of active metabolites from consuming pomegranate juice
in three different preparations. People who participate in this study will come in to the
Center for a screening visit, which includes a blood test and medical history. If you
qualify based on the screening visit, you will then come in to the Center while fasting in
the morning on three different days. You will eat a standardized breakfast and drink one of
three juices, and then remain for metabolic testing at the Center for a period of time after
eating. You will also be provided a standardized lunch and water during this period. Each of
those visits will last about 7 hours. During this time, and for the rest of the 24 hours
following drinking the juice, you will collect all of your urine. Following all three of
these 7-hour visits, you will return to the Center again the next day for a fasting blood
draw, drop off your urine sample, and pick up a new container to collect another 24 hour
urine sample. The following day, you will return to the Center to drop off your second urine
sample collection.
in three different preparations. People who participate in this study will come in to the
Center for a screening visit, which includes a blood test and medical history. If you
qualify based on the screening visit, you will then come in to the Center while fasting in
the morning on three different days. You will eat a standardized breakfast and drink one of
three juices, and then remain for metabolic testing at the Center for a period of time after
eating. You will also be provided a standardized lunch and water during this period. Each of
those visits will last about 7 hours. During this time, and for the rest of the 24 hours
following drinking the juice, you will collect all of your urine. Following all three of
these 7-hour visits, you will return to the Center again the next day for a fasting blood
draw, drop off your urine sample, and pick up a new container to collect another 24 hour
urine sample. The following day, you will return to the Center to drop off your second urine
sample collection.
Pomegranate (Punica granatum L.) fruits are widely consumed as pomegranate juice (PJ), and
their health benefits have been studied extensively in animals and humans (1-6). The
pomegranate fruit is a rich source of polyphenols, most of which are ellagitannins (ETs).
Pomegranate extracts,which incorporate these major polyphenols from the pomegranate fruit,
have been developed in dry and liquid forms to provide alternative convenient sources for
obtaining the bioactive polyphenols found in PJ. We have previously reported on the
pharmacokinetics and metabolism of pomegranate polyphenols, after the consumption of PJ in
normal healthy human volunteers (7). In humans, ETs are converted to ellagic acid (EA) in
the small intestines and absorbed with a maximum plasma concentration observed at
approximately 1 hour. EA disappears from plasma 6 hours after administration of PJ.
Ellagitannins and ellagic acid remaining in the intestine and are then further converted by
gut microflora to urolithin-A, absorbed and derivatives, which are metabolized by phase II
enzymes and excreted in human urine for up to 48 hours after consumption of PJ(7).
Polyphenols are dietary constituents of plants associated with health-promoting effects. In
the human diet, polyphenols are generally consumed in foods along with macronutrients.
Because the health benefits of polyphenols are critically determined by their
bioavailability, the effect of interactions between plant phenols and food macronutrients is
a very important topic. The protein-polyphenol complexes can significantly change the plasma
kinetics profile. (8) Protein-polyphenol binding is mediated by a combination of hydrogen
and hydrophobic bonding depending on chemical (polarity) and structural (size/shape)
properties of interacting molecules. Covalent interactions between purified glycinin, a
soybean storage protein, and selected flavonoids and phenolic acids have also been reported.
Recently it is reported that protein-rich defatted soybean flour protects anthocyanins
during transit through upper digestive tract for subsequent colonic delivery/metabolism (9).
their health benefits have been studied extensively in animals and humans (1-6). The
pomegranate fruit is a rich source of polyphenols, most of which are ellagitannins (ETs).
Pomegranate extracts,which incorporate these major polyphenols from the pomegranate fruit,
have been developed in dry and liquid forms to provide alternative convenient sources for
obtaining the bioactive polyphenols found in PJ. We have previously reported on the
pharmacokinetics and metabolism of pomegranate polyphenols, after the consumption of PJ in
normal healthy human volunteers (7). In humans, ETs are converted to ellagic acid (EA) in
the small intestines and absorbed with a maximum plasma concentration observed at
approximately 1 hour. EA disappears from plasma 6 hours after administration of PJ.
Ellagitannins and ellagic acid remaining in the intestine and are then further converted by
gut microflora to urolithin-A, absorbed and derivatives, which are metabolized by phase II
enzymes and excreted in human urine for up to 48 hours after consumption of PJ(7).
Polyphenols are dietary constituents of plants associated with health-promoting effects. In
the human diet, polyphenols are generally consumed in foods along with macronutrients.
Because the health benefits of polyphenols are critically determined by their
bioavailability, the effect of interactions between plant phenols and food macronutrients is
a very important topic. The protein-polyphenol complexes can significantly change the plasma
kinetics profile. (8) Protein-polyphenol binding is mediated by a combination of hydrogen
and hydrophobic bonding depending on chemical (polarity) and structural (size/shape)
properties of interacting molecules. Covalent interactions between purified glycinin, a
soybean storage protein, and selected flavonoids and phenolic acids have also been reported.
Recently it is reported that protein-rich defatted soybean flour protects anthocyanins
during transit through upper digestive tract for subsequent colonic delivery/metabolism (9).
Inclusion Criteria:
- Male or female >20 and < 45 years of age at the time the consent form is signed.
- Subject must sign the Institutional Review Board-approved written informed consent
prior to the initiation of any study specific procedures or randomization. A subject
will be excluded for any condition that might compromise the ability to give truly
informed consent for participation in the study.
Exclusion Criteria:
- Any subject who is eating more than 4 servings per day of fruits and vegetables,
taking vitamin supplements or taking any antibiotics or other medication or dietary
supplement which interfere with the absorption of polyphenols.
- Any subjects with a history of gastrointestinal surgery (except for appendectomy),
diabetes mellitus, or other serious medical condition, such as chronic hepatitis or
renal disease, bleeding disorder, congestive heart disease, chronic diarrhea
disorders, myocardial infarction, coronary artery bypass graft, angioplasty within 6
months prior to screening, current diagnosis of uncontrolled hypertension (defined as
BP >160mmHg, diastolic BP>95mmHg), active or chronic gastrointestinal disorders,
bulimia, anorexia, laxative abuse, or endocrine diseases (except thyroid disease
corrected with medication to normal laboratory values) as indicated by medical
history or routine physical examination.
- Major surgery within 12 weeks prior to subject randomization and/or screening,
especially cardiac surgery
- Is currently a smoker who has a therapeutic plan to quit smoking anytime during the
study period; or if not a current smoker, has quit smoking within the past 3 months.
- Known HIV positive.
- Clinical evidence of current malignancy with the exception of basal cell or squamous
cell carcinoma of the skin and cervical intraepithelial neoplasia.
- Currently receiving systemic chemotherapy and/or radiotherapy.
- Active bleeding.
- Subject has any disorder (excluding illiteracy or visual impairment) that compromises
the ability of the subject to give written informed consent and/or to comply with
study procedures.
- Subject who is allergic to soy protein.
- In the opinion of the study investigator has a risk of non-compliance with study
procedures, or can not read, understand or complete study related materials.
We found this trial at
1
site
Click here to add this to my saved trials
