The Effect of Pomegranate on Aging and Inflammation of the Skin

Pomegranate (Punica granatum L.) is grown commercially in the Near East, India, Spain,
Israel and the United States (California) where it is of significant economic importance1.
Pomegranate fruits and products, including juice, tea, wine and extracts are widely consumed
and recognized for their health benefits. Pomegranate (Punica granatum L.) fruit possesses
strong antioxidant, anti-inflammatory and antiproliferative properties. In recent years,
most health advantages of pomegranate have been attributed to the presence of ellagitannins,
mainly punicalagins and ellagic acid. Punicalagin is unique to pomegranate and is part of a
family of ellagitannins. The ellagitannins are hydrolyzed to ellagic acid in the gut, and
further metabolized by the colon microflora to form urolith A and B. Investigations using
pure bacterial cultures have shown that pomegranate by-products and punicalagins
significantly inhibited the growth of pathogenic Escherichia coli, Pseudomonas aeruginosa,
clostridia and Staphylococcus aureus.

Oral feeding of pomegranate fruit extract to mice afforded protection to mouse skin against
the adverse effects of ultraviolet-B (UVB) radiation by modulating UVB-induced signaling
pathways.5 Hydroalcoholic extract based-ointment from pomegranate was reported improving
wound healing in vivo. Pomegranate ellagitannins have been demonstrated to have
antimicrobial activity. Punicalagin is unique to pomegranate and is part of a family of
ellagitannins. The ellagitannins are hydrolyzed to ellagic acid in the gut, and further
metabolized by the colon microflora to form urolith A and B. Investigations using pure
bacterial cultures have shown that pomegranate by-products and punicalagins significantly
inhibited the growth of pathogenic Escherichia coli, Pseudomonas aeruginosa, clostridia and
Staphylococcus aureus.

Pomegranate Extract (POMx) is made from pomegranate fruit as a byproduct of pomegranate
juice (PJ) production. A second pressing of the fruit liberates a complex mixture of
hydrolysable polyphenolic compounds normally ingested with pomegranate juice, and these are
purified by spray drying. POMx powder is encapsulated for oral administration, with each
capsule containing 1,000 mg of pomegranate polyphenols. The present study will determine
whether a pomegranate product (POMx or PJ) can decrease skin photoaging, inflammation and
skin pathogenic bacteria.

Inclusion Criteria:

1. Age 30-45 years or older, female and any racial/ethnic group

2. Subjects must understand and sign the informed consent prior to participation

3. Subjects must be in generally good health

4. Subjects must be able and willing to comply with the requirements of the protocol.

Exclusion Criteria:

1. Oral or topical retinoid use within twelve months of entry into the study

2. Non-compliant subjects

3. Taking any antibiotics or other medication or dietary supplements regularly

4. Subjects with a significant medical history or concurrent condition that the
investigator(s) feel is not safe for the study

5. Subjects who cannot avoid excessive exposure to either natural or artificial
sunlight.

6. Allergic to pomegranate products