Resveratrol and Human Hepatocyte Function in Cancer
| Status: | Withdrawn |
|---|---|
| Conditions: | Liver Cancer, Cancer, Cancer |
| Therapuetic Areas: | Oncology |
| Healthy: | No |
| Age Range: | 21 - 80 |
| Updated: | 6/15/2016 |
| Start Date: | December 2015 |
| End Date: | June 2016 |
The purpose of this study is to determine if Resveratrol, a nutritional supplement, shows a
beneficial effect in the cellular function of normal liver cells and diseased liver cells
(cancer cells) in samples of liver tissue taken during elective liver surgery. Outcomes
based on 3 measures will test the hypothesis that Resveratrol when used as a nutritional
supplement will 1)improve metabolic function in liver cells, 2)reduce cellular growth and
proliferation of cancer cells, 3)decrease inflammation in the liver.
beneficial effect in the cellular function of normal liver cells and diseased liver cells
(cancer cells) in samples of liver tissue taken during elective liver surgery. Outcomes
based on 3 measures will test the hypothesis that Resveratrol when used as a nutritional
supplement will 1)improve metabolic function in liver cells, 2)reduce cellular growth and
proliferation of cancer cells, 3)decrease inflammation in the liver.
Hepatic function will be assessed by standard laboratory techniques. Hepatocyte signaling
pathway proteins will be measured using western blot analysis for protein expression and
polymerase chain reaction for gene expression. Activation of signaling pathways in both
native hepatocytes and carcinoma will be analyzed by multi-plex signal array. The effect on
transcription factors that may be important in gene expression will be analyzed by
transcription factor array. The effect of resveratrol in altering hepatocyte and cancer cell
metabolism will be analyzed by proteomic analysis.
pathway proteins will be measured using western blot analysis for protein expression and
polymerase chain reaction for gene expression. Activation of signaling pathways in both
native hepatocytes and carcinoma will be analyzed by multi-plex signal array. The effect on
transcription factors that may be important in gene expression will be analyzed by
transcription factor array. The effect of resveratrol in altering hepatocyte and cancer cell
metabolism will be analyzed by proteomic analysis.
Inclusion Criteria:
- Undergoing elective liver resection for liver cancer
Exclusion Criteria:
- Inability to speak or read English
- Sclerosing cholangitis, hemochromatosis, hepatic encephalopathy, acute hepatic
failure
- History of daily alcohol intake
- Presence of human immunodeficiency virus
- Presence of significant renal dysfunction as defined by baseline serum creatinine >
2.0 mg/dl or need/impending need for chronic dialysis therapy
- Known allergy to the study medication
- Pregnancy, lactating women, women contemplating pregnancy during the study period
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