Evaluating the Safety and Immune Response to Three Different DNA HIV Vaccines Administered With a MVA-CMDR Boost Vaccine in Healthy, HIV-Uninfected Adults

This study will evaluate the safety, tolerability, and immunogenicity to four different HIV
vaccines in healthy, HIV-uninfected adults. The vaccines include three DNA vaccines—DNA Nat-B
env, DNA CON-S env, and DNA Mosaic env—and a vaccine called MVA-CMDR that may boost the
immune response to the DNA vaccines.

The study will enroll healthy, HIV-uninfected participants aged 18 to 50 years. Participants
will be randomly assigned to one of three groups and will receive either one of the
experimental vaccine regimens or a placebo vaccine regimen. Group 1 participants will receive
the DNA Nat-B env and MVA-CMDR vaccines or placebo. Group 2 participants will receive the DNA
CON-S env and MVA-CMDR vaccines or placebo. Group 3 participants will receive DNA Mosaic env
and MVA-CMDR vaccines or placebo.

All participants will receive one of their assigned vaccines at study entry (Month 0), and
Months 1, 2, 4, and 8.

Total study duration will be either 3 years after enrollment (for participants in the United
States) or 5 years after enrollment (for participants in Switzerland). For all participants,
study visits will occur at study entry (Month 0), and Months 0.5, 1, 1.5, 2, 2.5, 4, 4.5, 8,
8.25, 8.5, 11, 13.75, and 14. After the last study visit, participants will be contacted
annually by phone or e-mail for a total of 3 (U.S. participants) or 5 (Switzerland
participants) years to answer questions about their health.

All study visits will include a physical exam, HIV risk reduction counseling, and an
interview and/or questionnaire. Select study visits will include blood collection, urine and
stool collection, HIV testing, an electrocardiogram (ECG), and a pregnancy test for
participants who were born female. For participants receiving the MVA-CMDR vaccine, select
study visits may also include an assessment of cardiac symptoms.

Inclusion Criteria:

General and Demographic Criteria

- Access to a participating HIV Vaccine Trials Network (HVTN) clinical research site
(CRS) and willingness to be followed for the planned duration of the study

- Ability and willingness to provide informed consent

- Assessment of understanding: participant demonstrates understanding of this study;
completes a questionnaire prior to first vaccination with verbal demonstration of
understanding of all questionnaire items answered incorrectly

- Willing to be contacted annually after completion of scheduled clinic visits for a
total of 3 years for U.S. participants (5 years for participants in Switzerland)
following initial study injection

- Agrees not to enroll in another study of an investigational research agent

- Good general health as shown by medical history, physical exam, and screening
laboratory tests

HIV-Related Criteria

- Willingness to receive HIV test results

- Willingness to discuss HIV infection risks and amenable to HIV risk reduction
counseling

- Assessed by the clinic staff as being at "low risk" for HIV infection and committed to
maintaining behavior consistent with low risk of HIV exposure through the last
required protocol clinic visit

Laboratory Inclusion Values

Hemogram/Complete Blood Count (CBC)

- Hemoglobin greater than or equal to 12.5 g/dL for participants who were born female,
greater than or equal to 13.5 g/dL for participants who were born male

- White blood cell count equal to 3,300 to 12,000 cells/mm^3

- Total lymphocyte count greater than or equal to 800 cells/mm^3

- Remaining differential either within institutional normal range or with site physician
approval

- Platelets equal to 125,000 to 550,000/mm^3

Chemistry

- Chemistry panel: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and
alkaline phosphatase less than 1.25 times the institutional upper limit of normal;
creatinine less than or equal to the institutional upper limit of normal

- Cardiac Troponin T or I (cTnT or cTnI) does not exceed the institutional upper limit
of normal

Virology

- Negative HIV-1 and -2 blood test: U.S. participants must have a negative Food and Drug
Administration (FDA)-approved enzyme immunoassay (EIA). Non-U.S. site may use locally
available assays that have been approved by HVTN Laboratory Operations.

- Negative hepatitis B surface antigen (HBsAg)

- Negative anti-hepatitis C virus antibodies (anti-HCV), or negative HCV polymerase
chain reaction (PCR) if the anti-HCV is positive

Urine

- Normal urine:

- Negative urine glucose, and

- Negative or trace urine protein, and

- Negative or trace urine hemoglobin (if trace hemoglobin is present on dipstick, a
microscopic urinalysis with red blood cells levels within institutional normal
range)

Reproductive Status

- Participants who were born female: negative serum or urine beta human chorionic
gonadotropin (beta-HCG) pregnancy test performed prior to vaccination on the day of
initial vaccination. Persons who are NOT of reproductive potential due to having
undergone total hysterectomy or bilateral oophorectomy (verified by medical records),
are not required to undergo pregnancy testing.

- Reproductive status: participants who were born female must:

- Agree to consistently use effective contraception (information on effective
contraception methods can be found in the protocol) for sexual activity that
could lead to pregnancy from at least 21 days prior to enrollment through the
last required protocol clinic visit;

- Or not be of reproductive potential, such as having reached menopause (no menses
for 1 year) or having undergone hysterectomy, bilateral oophorectomy, or tubal
ligation;

- Or be sexually abstinent.

- Participants who were born female must also agree not to seek pregnancy through
alternative methods, such as artificial insemination or in vitro fertilization until
after the last required protocol clinic visit.

Exclusion Criteria:

General

- Blood products received within 120 days before first vaccination

- Investigational research agents received within 30 days before first vaccination

- Body mass index (BMI) greater than or equal to 40

- Intent to participate in another study of an investigational research agent during the
planned duration of the HVTN 106 study

- Pregnant or breastfeeding

Vaccines and Other Injections

- Smallpox vaccine received within the last 5 years

- HIV vaccine(s) received in a prior HIV vaccine trial. For participants who have
received control/placebo in an HIV vaccine trial, the HVTN 106 Protocol Safety Review
Team (PSRT) will determine eligibility on a case-by-case basis and the identity of the
study control/placebo must be obtained.

- Non-HIV experimental vaccine(s) received within the last 5 years in a prior vaccine
trial. Exceptions may be made for vaccines that have subsequently undergone licensure
by the FDA. For participants who have received control/placebo in an experimental
vaccine trial, the HVTN 106 PSRT will determine eligibility on a case-by-case basis.
For participants who have received an experimental vaccine(s) more than 5 years ago,
eligibility for enrollment will be determined by the HVTN 106 PSRT on a case-by-case
basis.

- Live attenuated vaccines other than influenza vaccine received within 30 days before
first vaccination or scheduled within 14 days after injection (e.g., measles, mumps,
and rubella [MMR]; oral polio vaccine [OPV]; varicella; yellow fever)

- Influenza vaccine or any vaccines that are not live attenuated vaccines and were
received within 14 days prior to first vaccination (e.g., tetanus, pneumococcal,
hepatitis A or B)

- Allergy treatment with antigen injections within 30 days before first vaccination or
that are scheduled within 14 days after first vaccination

Immune System

- Immunosuppressive medications received within 168 days before first vaccination. (Not
excluded: [1] corticosteroid nasal spray; [2] inhaled corticosteroids; [3] topical
corticosteroids for mild, uncomplicated dermatitis; or [4] a single course of
oral/parenteral corticosteroids at doses less than 2 mg/kg/day and length of therapy
less than 11 days with completion at least 30 days prior to enrollment

- Serious adverse reactions to vaccines or to vaccine components such as neomycin or
streptomycin, including anaphylaxis and related symptoms such as hives, respiratory
difficulty, angioedema, and/or abdominal pain. (Not excluded: a participant who had a
nonanaphylactic adverse reaction to pertussis vaccine as a child)

- Immunoglobulin received within 60 days before first vaccination

- Autoimmune disease

- Immunodeficiency

- Hypersensitivity to eggs and/or egg products

Clinically Significant Medical Conditions

- Untreated or incompletely treated syphilis infection

- Clinically significant medical condition, physical examination findings, clinically
significant abnormal laboratory results, or past medical history with clinically
significant implications for current health. More information on this criterion can be
found in the protocol.

- Any medical, psychiatric, occupational, or other condition that, in the judgment of
the investigator, would interfere with, or serve as a contraindication to, protocol
adherence, assessment of safety or reactogenicity, or a participant's ability to give
informed consent

- Psychiatric condition that precludes compliance with the protocol. Specifically
excluded are persons with psychoses within the past 3 years, ongoing risk for suicide,
or history of suicide attempt or gesture within the past 3 years.

- Current anti-tuberculosis (TB) prophylaxis or therapy

- Asthma exclusion criteria: Asthma other than mild, well-controlled asthma. More
information on this criterion can be found in the protocol.

- Diabetes mellitus type 1 or type 2, including cases controlled with diet alone (Not
excluded: history of isolated gestational diabetes)

- Thyroidectomy, or thyroid disease requiring medication during the last 12 months

- Hypertension:

- If a person has been found to have elevated blood pressure or hypertension during
screening or previously, exclude for blood pressure that is not well controlled.
Well-controlled blood pressure is defined as consistently less than or equal to
140 mm Hg systolic and less than or equal to 90 mm Hg diastolic, with or without
medication, with only isolated, brief instances of higher readings, which must be
less than or equal to 150 mm Hg systolic and less than or equal to 100 mm Hg
diastolic. For these participants, blood pressure must be less than or equal to
140 mm Hg systolic and less than or equal to 90 mm Hg diastolic at enrollment.

- If a person has NOT been found to have elevated blood pressure or hypertension
during screening or previously, exclude for systolic blood pressure greater than
or equal to 150 mm Hg at enrollment or diastolic blood pressure greater than or
equal to 100 mm Hg at enrollment.

- History of myocarditis, pericarditis, cardiomyopathy, congestive heart failure with
permanent sequelae, clinically significant arrhythmia (including any arrhythmia
requiring medication, treatment, or clinical follow-up)

- Participants who have 2 or more of the following cardiac risk factors:

- Participant report of history of elevated blood cholesterol defined as fasting
low-density lipoprotein (LDL) greater than 160 mg/dL;

- First degree relative (e.g., mother, father, brother, or sister) who had coronary
artery disease before the age of 50 years;

- Current smoker; or

- BMI greater than or equal to 35

- Electrocardiogram (ECG) with clinically significant findings, or features that would
interfere with the assessment of myo/pericarditis, as determined by the contract ECG
Lab, cardiologist, or study clinician. More information on this criterion can be found
in the protocol.

- History of hereditary angioedema, acquired angioedema, or idiopathic angioedema

- Bleeding disorder diagnosed by a doctor (e.g., factor deficiency, coagulopathy, or
platelet disorder requiring special precautions)

- Malignancy (Not excluded: participant who has had malignancy excised surgically and
who, in the investigator's estimation, has a reasonable assurance of sustained cure or
who is unlikely to experience recurrence of malignancy during the period of the study)

- Seizure disorder: History of seizure(s) within past 3 years. Also exclude if
participant has used medications in order to prevent or treat seizure(s) at any time
within the past 3 years.

- Asplenia: any condition resulting in the absence of a functional spleen