Brassica Intake and Isothiocyanate Absorption
| Status: | Completed |
|---|---|
| Conditions: | Healthy Studies |
| Therapuetic Areas: | Other |
| Healthy: | No |
| Age Range: | 21 - 70 |
| Updated: | 4/13/2015 |
| Start Date: | January 2015 |
| End Date: | April 2015 |
Brassica Intake and Isothiocyanate Absorption: Intake Patterns May Have Implications for Cancer Prevention by Dietary Brassica Vegetables
Healthy individuals (n=18) will participate in 2 controlled-feeding study periods that are 3
weeks each in length: 1) Control diet 2) Brassica diet. The control diet will consist of
typical American foods and will be free of Brassica vegetables and free of glucosinolates
and isothiocyanates. The Brassica diet will contain the control diet plus
glucosinolate/isothiocyanate treatment foods. There will be a break of 3 weeks in between
study periods. Blood, urine, and fecal samples will be collected at the end of each study
period. Eligible participants will be selected based on genotype for glutathione
S-transferase (GST), which has been shown to influence the potential protective role of
dietary Brassicas. Half the participants (n=9) will be GSTM1-positive individuals and half
(n=9) will have the GSTM1-null genotype.
weeks each in length: 1) Control diet 2) Brassica diet. The control diet will consist of
typical American foods and will be free of Brassica vegetables and free of glucosinolates
and isothiocyanates. The Brassica diet will contain the control diet plus
glucosinolate/isothiocyanate treatment foods. There will be a break of 3 weeks in between
study periods. Blood, urine, and fecal samples will be collected at the end of each study
period. Eligible participants will be selected based on genotype for glutathione
S-transferase (GST), which has been shown to influence the potential protective role of
dietary Brassicas. Half the participants (n=9) will be GSTM1-positive individuals and half
(n=9) will have the GSTM1-null genotype.
Inclusion Criteria:
- Between 21 - 70 years of age
- Not currently taking glucosinolate/ITC containing supplements
- Voluntarily agree to participate and sign an informed consent document
- Free of cancer (never diagnosed or cancer-free for at least 5 y)
Exclusion Criteria:
- Pregnant, lactating, or intend to become pregnant during the study period
- Known allergy or intolerance to Brassica vegetables
- History of bariatric surgery or nutrient malabsorption disease (such as celiac
disease) or other metabolic disorders requiring special diet recommendations
- Use of tobacco products
- Use of certain medications (prescription or over-the-counter) that may interfere with
the study objectives
- Type 2 diabetes requiring the use of diabetes pills, insulin, or non-insulin shots
- Use of blood-thinning medications such as Coumadin (warfarin), Dicumarol, or Miradon
(anisindione)
- Unable or unwilling to give informed consent or communicate with study staff
- Self-report of alcohol or substance abuse within the past 12 months and/or current
acute treatment or rehabilitation program for these problems (long-term participation
in Alcoholics Anonymous is not an exclusion)
- Other medical, psychiatric, or behavioral factors that in the judgment of the
Principal Investigator may interfere with study participation or the ability to
follow the intervention protocol
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