Phase II Trial of Dasatinib in Patients With Isocitrate Dehydrogenase (IDH)-Mutant Advanced Intrahepatic Cholangiocarcinoma

This research study is a Phase II clinical trial. Phase II clinical trials test the safety
and effectiveness of an investigational intervention to learn whether the intervention works
in treating a specific disease. "Investigational" means that the intervention is being
studied.

The standard treatment for metastatic cholangiocarcinoma is combination chemotherapy with
gemcitabine and cisplatin. The FDA (the U.S. Food and Drug Administration) has not approved
dasatinib for your specific disease (Cholangiocarcinoma) but it has been approved for other
uses (Chronic Myeloid Leukemia)

Inclusion Criteria:

- Participants must meet the following criteria on screening examination to be eligible
to participate in the study:

- Participants must have unresectable or metastatic histologically confirmed
intrahepatic cholangiocarcinoma

- Patients must have either IDH1 or IDH2 mutations (any known mutations) based on the
SNaPshot platform or other molecular testing platform from either archived tissue or
fresh biopsy (tested in CLIA-certified lab)

- Patients with other biliary tract cancers (extrahepatic or gallbladder cancers) with
IDH1 or IDH2 mutations are allowed

- Participants must have measurable disease, defined as at least one lesion that can be
accurately measured in at least one dimension (longest diameter to be recorded) as ≥
20 mm with conventional techniques or as ≥10 mm with spiral CT scan. See section 10
for the evaluation of measureable disease.

- Participants must have received at least one prior platinum-based regimen for advanced
cholangiocarcinoma and had progressive disease or become intolerable to the regimen

- Age ≥18 years.

- Life expectancy of ≥3 months.

- ECOG performance status 0 or 1 (see Appendix A).

- Participants must have adequate organ and marrow function as defined below:

- Absolute neutrophil count ≥ 1,200/mcL

- Platelets ≥75,000/mcL

- Hemoglobin ≥9 g/dL

- Total bilirubin ≤ 2.5 x the upper limit of normal

- AST (SGOT)/ALT (SGPT) ≤ 5 X institutional upper limit of normal

- PT/PTT ≤ 1.5 x ULN

- Creatinine ≤ 1.5 or GFR ≥ 60 mL/min/1.73m2

- Serum Albumin ≥2.8 g/dl

- Prior chemoembolization, radiofrequency ablation, or radiation to the liver is allowed
as long as the patient has measurable disease outside of the treated area or
measurable progression at the site of the treated area

- Ability to understand and the willingness to sign a written informed consent document.

- Sexually active subjects (men and women) must agree to use medically accepted barrier
methods of contraception (eg, male or female condom) during the course of the study
and for 4 months after the last dose of study drug(s), even if oral contraceptives are
also used. All subjects of reproductive potential must agree to use both a barrier
method and a second method of birth control during the course of the study and for 4
months after the last dose of study drug(s).

- Women of childbearing potential must have a negative pregnancy test at screening.
Women of childbearing potential include women who have experienced menarche and who
have not undergone successful surgical sterilization (hysterectomy, bilateral tubal
ligation, or bilateral oophorectomy) or are not postmenopausal. Postmenopause is
defined as amenorrhea ≥ 12 consecutive months. Note: women who have been amenorrheic
for 12 or more months are still considered to be of childbearing potential if the
amenorrhea is possibly due to prior chemotherapy, antiestrogens, ovarian suppression
or any other reversible reason.

Exclusion Criteria:

- Participants who exhibit any of the following conditions at screening will not be
eligible for admission into the study.

- Prior treatment with dasatinib

- Periampullary tumors

- Chemotherapy, within 4 weeks prior to entering the study (6 weeks for nitrosoureas or
mitomycin) or those who have not recovered to less than or equal to grade 1 from
adverse events due to agents administered more than 4 weeks earlier.

- The subject has received radiation therapy:

- to bone or brain metastasis within 14 days of the first dose of study treatment

- to any other site(s) within 28 days of the first dose of study treatment

- The subject has active brain metastases or epidural disease (Note: Subjects with brain
metastases previously treated with whole brain radiation or radiosurgery or subjects
with epidural disease previously treated with radiation or surgery who are
asymptomatic and do not require steroid treatment for at least 2 weeks before starting
study treatment are eligible. Neurosurgical resection of brain metastases or brain
biopsy is permitted if completed at least 3 months before starting study treatment.
(Baseline brain imaging with contrast-enhanced CT or MRI scans for subjects with known
brain metastases is required to confirm eligibility.)

- The subject has uncontrolled, significant intercurrent or recent illness including,
but not limited to, the following conditions:

- Cardiovascular disorders including

- Congestive heart failure (CHF): New York Heart Association (NYHA) Class III
(moderate) or Class IV (severe) at the time of screening

- Concurrent uncontrolled hypertension defined as sustained BP > 140 mm Hg
systolic, or > 90 mm Hg diastolic despite optimal antihypertensive treatment
within 7 days of the first dose of study treatment

- Diagnosed or suspected congenital long QT syndrome

- Any of the following within 6 months before the first dose of study
treatment:

- Unstable angina pectoris

- Clinically-significant cardiac arrhythmias

- Stroke (including TIA, or other ischemic event)

- Myocardial infarction

- Thromboembolic event requiring therapeutic anticoagulation (Note:
subjects with a venous filter (e.g. vena cava filter) are not eligible
for this study)

- Any history of clinically significant ventricular arrhythmias (such as
ventricular tachycardia, ventricular fibrillation, or Torsades de
pointes)

- Prolonged QTc interval on pre-entry electrocardiogram (> 450 msec), may
use either the Fridericia and Bazett's correction

- Hypokalemia or hypomagnesemia that is not corrected prior to dasatinib
administration

- Patients should not be taking drugs that are generally accepted to have
a risk of causing Torsades de Pointes. The following must be
discontinued at least 7 days prior to starting dasatinib to be
eligible:quinidine, procainamide, disopyramide, amiodarone, sotalol,
ibutilide,dofetilide, erythromycins, clarithromycin, chlorpromazine,
haloperidol, mesoridazine, thioridazine, pimozide, cisapride, bepridil,
droperidol, methadone, arsenic, chloroquine, domperidone,
halofantrine,levomethadyl, pentamidine, sparfloxacin, lidoflazine

- Other clinically significant disorders such as:

- active infection requiring systemic treatment within 28 days before the
first dose of study treatment

- serious non-healing wound/ulcer/bone fracture within 28 days before the
first dose of study treatment

- Patients with known moderate/severe pleural effusions that are unrelated to
malignancy or established diagnosis of pulmonary arterial hypertension

- Concurrent malignancy (other than adequately treated non-melanoma skin cancer,
superficial transitional cell carcinoma of the bladder, and cervical carcinoma in
situ) diagnosed within the past 3 years or any currently active malignancy

- Psychiatric illness/social situations that would limit compliance with study
requirements.

- The subject has received any other type of investigational agent within 28 days before
the first dose of study treatment.

- The subject is unable to swallow tablets

- Individuals who are known to be HIV-positive are excluded from this study.

- Pregnant women are excluded from this study due to the potential for teratogenic or
abortifacient effects. Because there is an unknown but potential risk of adverse
events in nursing infants secondary to treatment of the mother with dasatinib,
breastfeeding should be discontinued if the mother is treated with dasatinib. These
potential risks may also apply to other agents used in this study.

- Subjects may not be receiving any other study agents concurrently while on this study