Safety and Efficacy of CG0070 Oncolytic Virus Regimen for High Grade NMIBC After BCG Failure

The plan is to study the safety and efficacy of CG0070 in high-grade NMIBC patients who
failed BCG therapy. Most patients with NMIBCis (Cis, Cis with Ta and/or T1, high grade Ta or
T1 with frequent or uncontrolled recurrences) who have failed BCG intravesical therapy
(standard of care) usually have no other choice but to proceed to cystectomy. Cystectomy is a
surgery associated with major morbidity, mortality and quality of life issues. Morbidity and
long term tedious medical care will be for the rest of the patient's life span. Most patients
at this stage do not show signs of disease progression into the muscle layer or of
metastasis, making surgery a very difficult decision. CG0070, if successful in this trial,
will serve to provide a therapeutic alternative for this patient population in need.

Inclusion Criteria:

1. Patients must have pathologically confirmed non-muscle invasive bladder cancer (NMIBC)
high grade disease (HG), as defined by the 2004 WHO classification system

2. Patients must have no evidence of muscle invasive disease

3. Patients must be able to provide a sufficient biopsy sample to the central pathologist
for histopathologically confirmed, transitional cell (urothelial) carcinoma.
Urothelial tumors with mixed histology (but with <50% variant) are eligible.

4. Patients must have received at least two or more prior courses of intravesical therapy
per recommended schedules. BCG must have been one of the prior therapies administered.

5. Patients can have either failed BCG induction therapy within a six-month period or
have been successfully treated with BCG, but subsequently found to have recurrence.
The first standard course of intravesical BCG therapy must include at least six weekly
treatments (allowable range of instillations per course is 4-9). The second course of
BCG therapy must include at least two weekly treatments.

6. Patients have either Cis or Cis with Ta and/or T1 disease at enrollment or in the
past. For those patients with only Ta or T1 disease at enrollment AND with no history
of Cis, they must have

- disease recurrence either must occur within 12 months of the most recent
intravesical therapy of any kind, OR

- disease recurrence within 18 months of BCG maintenance OR

- disease recurrence within 24 months of BCG induction

- T1 patients need to have evidence of muscle included in their latest biopsy; and
if not a re-TURBT has to be done prior to enrollment

7. 18 years of age or older

8. Radical cystectomy has been declined by the patient in a signed special section of the
informed consent, whereby there is a clear explanation by the investigator to the
subject that a delay of cystectomy may increase his/her chance of disease progression,
the results of which may lead to serious and life threatening consequences.

9. Patients must be able to enter into the study within ten weeks of their most recent
diagnostic procedure, which is usually a diagnostic biopsy, a transurethral resection
of bladder tumor (TURBT) procedure or positive urine cytology.

10. Eastern Cooperative Oncology Group (ECOG) performance status <2.

11. Not pregnant or lactating

12. Patients with child bearing potential must agree to use adequate contraception

13. Agree to study specific informed consent and HIPAA authorization for release of
personal health information

14. Adequate baseline CBC, renal and hepatic function. Parameters described as WBC>3000
cells/mm^3, ANC>1,000 cells/mm^3, hemoglobin>9.5g/dL, and platelet count >100,000
cells/mm^3

- Adequate renal function: serum creatinine <2.5mg/dL

- Bilirubin, AST and ALT not more than 2 x Upper Limits of Normal

- PT/INR, PTT, and fibrinogen within institutional acceptable limits

- Absolute lymphocyte count ≥ 800/μL before the first dose of CG0070

Exclusion Criteria:

1. Previous systemic chemotherapy or radiation for bladder cancer. Note: Prior
immunotherapy or intravesical (administered within the bladder) chemotherapy for
superficial disease is acceptable

2. History of anaphylactic reaction following exposure to humanized or human therapeutic
monoclonal antibodies, hypersensitivity to GM-CSF or yeast derived products,
clinically meaningful allergic reactions or any known hypersensitivity or prior
reaction to any of the formulation excipients in the study drugs.

3. Known infection with HIV, HBV or HCV.

4. Anticipated use of chemotherapy or radiotherapy not specified in the study protocol
while on study

5. Any underlying medical condition that, in the Investigator's opinion, will make the
administration of study vector hazardous to the patient, would obscure the
interpretation of adverse events, or not permit adequate surgical resection.

6. Systemic treatment on any investigational clinical trial within 28 days prior to
registration.

7. Concurrent treatment with immunosuppressive or immunomodulatory agents, including any
systemic steroid (exception: inhaled or topically applied steroids, and acute and
chronic standard dose NSAIDs, are permitted). Use of a short course (i.e., ≤ 1 day) of
a glucocorticoid is acceptable to prevent a reaction to the IV contrast used for CT
scans.

8. Immunosuppressive therapy, including: cyclosporine, antithymocyte globulin, or
tacrolimus within 3 months of study entry.

9. History of prior experimental cancer vaccine treatment (e.g., dendritic cell therapy,
heat shock vaccine) within the last year

10. History of stage III or greater cancer, excluding urothelial cancer. Basal or squamous
cell skin cancers must have been adequately treated and the subject must be
disease-free at the time of registration. Subjects with a history of stage I or II
cancer, must have been adequately treated and have been disease-free for ≥ 2 years at
the time of registration.

11. Progressive or persistent viral or bacterial infection

- All infections must be resolved and the patient must remain afebrile for seven
days without antibiotics prior to being placed on study

- Urinary tract infection, including particularly bladder infection, must be
resolved prior to being placed on study

12. Unwilling or unable to comply with the protocol or cooperate fully with the
investigator and site personnel