An Alternative Culture Medium to Better Support Preimplantation Embryo Development in Vitro
| Status: | Completed |
|---|---|
| Conditions: | Women's Studies, Infertility |
| Therapuetic Areas: | Reproductive |
| Healthy: | No |
| Age Range: | 18 - Any |
| Updated: | 8/30/2018 |
| Start Date: | May 2015 |
| End Date: | August 2018 |
The purpose of this research study is to evaluate a newly developed embryo culture media
system for human clinical use in ART.
system for human clinical use in ART.
Egg retrieval will be performed as per standard operating procedures. Eggs from a single
patient are mixed together in a single group during the normal recovery and washing procedure
following egg retrieval. The single group of eggs will be divided in half, then the
embryologist will randomly place each half into one of the two culture media treatments,
commercial or experimental. All mature eggs collected at retrieval will undergo fertilization
per standard operating procedures according to physician recommendations. After fertilization
(day 0), standard procedures involve in vitro embryo culture in a sequential two step media
system. After confirmation of fertilization success on day 1, embryos are cultured until day
3 in step 1 medium (both commercial and experimental media are two step sequential culture
systems). On day 3, embryos are evaluated and moved from step one to step two culture medium.
On day 5 and day 6, embryo development will be assessed. Good quality blastocysts will either
be transferred fresh or cryopreserved for later transfer and/or genetic testing.
patient are mixed together in a single group during the normal recovery and washing procedure
following egg retrieval. The single group of eggs will be divided in half, then the
embryologist will randomly place each half into one of the two culture media treatments,
commercial or experimental. All mature eggs collected at retrieval will undergo fertilization
per standard operating procedures according to physician recommendations. After fertilization
(day 0), standard procedures involve in vitro embryo culture in a sequential two step media
system. After confirmation of fertilization success on day 1, embryos are cultured until day
3 in step 1 medium (both commercial and experimental media are two step sequential culture
systems). On day 3, embryos are evaluated and moved from step one to step two culture medium.
On day 5 and day 6, embryo development will be assessed. Good quality blastocysts will either
be transferred fresh or cryopreserved for later transfer and/or genetic testing.
Inclusion Criteria:
- Adult women of any age who come to Colorado Center for Reproductive Medicine for
fertility treatment using IVF.
- Patients can be of any race, culture, sexual orientation or ethnicity.
Exclusion Criteria:
- Minors are excluded from the study
We found this trial at
2
sites
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