Early Feasibility Study of Zone-MPC and HMS With DiAs in the Outpatient Setting

This is a feasibility study of an artificial pancreas (AP) system with our previously
validated Zone-MPC and Health Monitoring System (HMS) algorithms (ClinicalTraisl.gov:
NCT01929798) integrated into the Diabetes Assistant (DiAs) system. The system will be
evaluated on 2-3 subjects per site (n=6-9 subjects) for 2 weeks at 3 different sites
(William Sansum Diabetes Center, University of Virginia, and Mayo Clinic, Rochester, MN).
Basal rates will be adjusted in a run-to-run manner by study physicians prior to the
closed-loop phase for a maximum of 3 weeks. Subjects will then complete 2 weeks at home use
of the Zone-MPC/HMS system using the DIAs platform for a closed-loop feasibility trial. The
purpose of this pilot study is to establish that safe day-and-night use of the Zone-MPC/HMS
system integrated into the DiAs is achievable in the home environment, to analyze and learn
to improve upon the run-to- run optimization process, and to collect efficacy data to inform
a future larger study.

Inclusion Criteria:

- Clinical diagnosis, based on investigator assessment, of type 1 diabetes for at least
one year and using insulin for at least 1 year and an insulin pump for at least 6
months

- Willing to wear the study CGM device for the duration of the study

- Age ≥21 to <65 years

- HbA1c <10.0%; if HbA1c <6.0% then total daily insulin must be ≥0.5 U/kg

- For females, not currently known to be pregnant If female and sexually active, must
agree to use a form of contraception to prevent pregnancy while a participant in the
study. A negative urine pregnancy test will be required for all premenopausal women
who are not surgically sterile. Subjects who become pregnant will be discontinued
from the study.

- Demonstration of proper mental status and cognition for the study

- Currently using insulin-to-carbohydrate ratio to calculate meal bolus sizes

- Hypoglycemia awareness as demonstrated by a Clarke Hypoglycemia Unawareness score of
2 or lower

- Access to internet and cell phone service at home, and a computer for downloading
device data

- Availability of care partner committed to participating in training activities,
knowledgeable at all times of the participant's location, and being present and
available to provide assistance when system is being used at night

- Commitment to maintaining uninterrupted availability via cell phone and avoiding any
overnight travel for the duration the period using the closed-loop system

- An understanding of and willingness to follow the protocol and sign the informed
consent

Exclusion Criteria:

- Admission for diabetic ketoacidosis in the 12 months prior to enrollment

- Severe hypoglycemia resulting in seizure or loss of consciousness in the 12 months
prior to enrollment

- History of a seizure disorder (except hypoglycemic seizure), unless written clearance
is received from a neurologist

- Coronary artery disease or heart failure, unless written clearance is received from a
cardiologist

- History of cardiac arrhythmia (except for benign premature atrial contractions and
benign premature ventricular contractions which are permitted)

- Cystic fibrosis

- A known medical condition that in the judgment of the investigator might interfere
with the completion of the protocol such as the following examples:

- Inpatient psychiatric treatment in the past 6 months for either the subject or
the subject's primary care giver (i.e., parent or guardian)

- Presence of a known adrenal disorder

- Abnormal liver function test results (Transaminase >2 times the upper limit of
normal); testing required for subjects taking medications known to affect liver
function or with diseases known to affect liver function

- Abnormal renal function test results (calculated GFR <60 mL/min/1.73m2); testing
required for subjects with diabetes duration of greater than 5 years post onset
of puberty

- Active gastroparesis

- If on antihypertensive, thyroid, anti-depressant or lipid lowering medication,
lack of stability on the medication for the past 2 months prior to enrollment in
the study

- Uncontrolled thyroid disease (TSH undetectable or >10 mlU/L); testing required
within three months prior to admission for subjects with a goiter or who are on
thyroid hormone replacement, and within one year otherwise

- Abuse of alcohol or recreational drugs

- Infectious process not anticipated to resolve prior to study procedures (e.g.
meningitis, pneumonia, osteomyelitis)

- A recent injury to body or limb, muscular disorder, use of any medication, any
carcinogenic disease, or other significant medical disorder if that injury,
medication or disease in the judgment of the investigator will affect the completion
of the protocol

- Current use of the following drugs and supplements:

- Acetaminophen

- Any medication being taken to lower blood glucose, such as Pramlintide,
Metformin,GLP-1 Analogs such as Liraglutide, and nutraceuticals intended to
lower blood glucose

- Beta blockers

- Oral or injectable glucocorticoids

- Any other medication that the investigator believes is a contraindication to the
subject's participation