Biomarkers in Tissue Samples From Patients Who Have Undergone Neck Dissection for Oral Cancer

OBJECTIVES:

- To test preliminary data from node-positive oral squamous cell carcinoma (OSCC)
signatures for their ability to predict lymph-positive primaries using a cohort of OSCC
tumors.

- To perform lymph node prediction using sentinel lymph node-biopsied primaries.

- To validate the preliminary data node-positive OSCC signature and test its ability to
predict nodal status.

OUTLINE: Patient tissue samples are screened using polymerase chain reaction (PCR) for human
papilloma virus-specific primers. Samples are analyzed to identify a nodal-metastasis
signature for oral squamous cell carcinoma. Samples also undergo microarray analysis to
quantify expression levels for targeted genes. Initial data analysis is performed using
Affymetrix® Microarray Suite 5.0 to quantify expression levels for targeted genes.

- Have a primary tumor specimen banked by the American College of Surgeons Oncology
Group (ACOSOG) as part of clinical trial ACOSOG-Z0360

* All tumor specimens are squamous cell carcinomas of the tongue, floor of the mouth,
or gingiva

- All patients have undergone neck dissections * Pathologically confirmed nodal stage
(negative or positive)