Pilot Study to Assess Safety and Feasibility of Normothermic Machine Preservation In Human Liver Transplantation

The shortage of donor organs leads to the use of marginal donors including donors after
cardiac death. Normothermic machine perfusion (NMP) preservation is a promising method to
decrease their high risk of complication compared to the standard cold storage (CS). The aim
of this project is to assess safety and feasibility of NMP in human liver transplantation.

This will be a single center prospective cohort pilot study. Thirty-two livers that have
acceptable quality for transplantation will be preserved with NMP in 2-18 hours after cross
clamp and cold flush. The liver grafts at NMP will be at physiological temperature and have
oxygen and nutrient supply with continuous perfusion. The transplantation and post-transplant
care will follow the standard of care. The follow-up period is 12 months after
transplantation. The primary end point will be the rate of post-transplant Early Allograft
Dysfunction (EAD), while the secondary end points will be: primary non function (PNF) rate, 1
and 6 months patient and graft survival, peak liver function tests in the first 7 days after
transplantation, intraoperative hepatic arterial and portal flow measurement, rate and
magnitude of post-reperfusion syndrome, surgical outcomes (operative time, transfusion
requirement etc.), rate of post-transplant kidney failure, assessment of histological
ischemia reperfusion (liver and bile duct), rate of vascular complications, rate of biliary
complications, hospital and ICU length of stay, rejection rate, infection rate, the ability
to predict function based on "on-pump" viability markers, and the incidence of adverse effect
(AE).

The outcome will be compared to a control group of 100 historical patients (matched with a
1:4 ratio) transplanted in our program in the past 10 years (liver preserved using CS).
Control subjects will be matched using donor and recipient age, Model for End-Stage Liver
Disease (MELD) score, cold ischemia time, donor risk index and presence of steatosis.

Inclusion Criteria:

- Patients undergoing primary liver transplantation

- Age 18-75 years at the time of transplantation

- Willingness and ability to comply with the study procedures

- Signed Informed Consent Form

- For patients with hepatocellular carcinoma as indication for Orthoptic Liver
Transplantation (OLT), tumor must be within Milan Criteria or down-staged to Milan
Criteria.

Exclusion Criteria:

Recipient Exclusion Criteria:

- History of prior solid organ transplantation

- Patient on a respiratory and/or cardiocirculatory support at the time of transplant

- MELD score >35

- HIV positive patient

- Patient with current severe systemic infection

- Multiorgan transplant

Donor Exclusion Criteria:

- Donor age <14 or >70 years

- Liver steatosis (on visual estimate or on liver biopsy) > 30%

- Donors with Grade 2-3 traumatic liver laceration

- Partial grafts (living donors and split liver donors)

- Liver in which the investigator is unwilling or unable (for logistical reasons) to
perform normothermic machine perfusion (NMP) preservation

- Inability to NMP perfuse the liver within 4 hours after cross clamp