Effect of Broccoli Sprout Extract on Keratinocyte Differentiation in Normal Skin
| Status: | Completed |
|---|---|
| Conditions: | Skin and Soft Tissue Infections |
| Therapuetic Areas: | Dermatology / Plastic Surgery |
| Healthy: | No |
| Age Range: | 18 - Any |
| Updated: | 12/8/2017 |
| Start Date: | September 2015 |
| End Date: | December 2016 |
Adult participants will apply a broccoli sprout extract-jojoba oil compound to one arm every
night under occlusion for 1 week. Jojoba oil alone will be applied to the other arm. At the
end of 1 week, a 6mm punch biopsy will be taken from both arms and analyzed via polymerase
chain reaction (PCR) and immunohistochemistry for differences in various skin proteins.
night under occlusion for 1 week. Jojoba oil alone will be applied to the other arm. At the
end of 1 week, a 6mm punch biopsy will be taken from both arms and analyzed via polymerase
chain reaction (PCR) and immunohistochemistry for differences in various skin proteins.
10 adult volunteers, including African American, Caucasian, male and female participants,
will be recruited from parents of patients seen in the pediatric dermatology clinic. Each
participant will receive 7ml of broccoli sprout extract (BSE) in jojoba oil, containing
3500nmol of sulforaphane (Please see the preparation of broccoli sprout extract below).
500nmol of sulforaphane will be applied each night. The participants will apply the
BSE-jojoba oil mixture over a circle, 3cm in diameter, on the upper arm every night for 7
consecutive nights. The control arm will have only jojoba oil applied. Both arms will be
occluded with saran wrap every night. After the seventh night, the volunteers will have a 6mm
biopsy taken from each arm (2 total), performed by a dermatology resident. The biopsies will
be cleaned with alcohol, numbed with 0.5-1cc of 1% lidocaine with epinephrine, and closed
with one 4-0 nylon nonabsorbable suture. The biopsy site will be covered with Vaseline and a
bandaid. The area will be kept covered for 24 hours, after which it may be washed with soap
and water and then recovered with Vaseline and a bandaid. The suture will be removed 10-14
days after the procedure. From the initial visit through the final visit for suture removal,
we expect the duration of the trial to last approximately 1 month. The participants will be
told to avoid applying any topical medications or over the counter lotions or creams for the
duration of the study and will also be told to wash with Dove soap in the shower. They will
also be advised to avoid a diet high in cruciferous plants such as broccoli, mustard, and
horseradish. The biopsy results will each be divided in half. One half to be frozen for
cryosectioning and immunofluorescence staining and the other half to be used for protein or
RNA extraction for molecular assays (real time polymerase chain reaction and/or quantitative
polymerase chain reaction for select messenger RNAs and western blotting for select protein
antigens). The levels of keratin 1, keratin 5, keratin 10, keratin 14, keratin 16, keratin
17, and Nrf2 will be studied with both immunofluorescence and RNA PCR.
will be recruited from parents of patients seen in the pediatric dermatology clinic. Each
participant will receive 7ml of broccoli sprout extract (BSE) in jojoba oil, containing
3500nmol of sulforaphane (Please see the preparation of broccoli sprout extract below).
500nmol of sulforaphane will be applied each night. The participants will apply the
BSE-jojoba oil mixture over a circle, 3cm in diameter, on the upper arm every night for 7
consecutive nights. The control arm will have only jojoba oil applied. Both arms will be
occluded with saran wrap every night. After the seventh night, the volunteers will have a 6mm
biopsy taken from each arm (2 total), performed by a dermatology resident. The biopsies will
be cleaned with alcohol, numbed with 0.5-1cc of 1% lidocaine with epinephrine, and closed
with one 4-0 nylon nonabsorbable suture. The biopsy site will be covered with Vaseline and a
bandaid. The area will be kept covered for 24 hours, after which it may be washed with soap
and water and then recovered with Vaseline and a bandaid. The suture will be removed 10-14
days after the procedure. From the initial visit through the final visit for suture removal,
we expect the duration of the trial to last approximately 1 month. The participants will be
told to avoid applying any topical medications or over the counter lotions or creams for the
duration of the study and will also be told to wash with Dove soap in the shower. They will
also be advised to avoid a diet high in cruciferous plants such as broccoli, mustard, and
horseradish. The biopsy results will each be divided in half. One half to be frozen for
cryosectioning and immunofluorescence staining and the other half to be used for protein or
RNA extraction for molecular assays (real time polymerase chain reaction and/or quantitative
polymerase chain reaction for select messenger RNAs and western blotting for select protein
antigens). The levels of keratin 1, keratin 5, keratin 10, keratin 14, keratin 16, keratin
17, and Nrf2 will be studied with both immunofluorescence and RNA PCR.
Inclusion Criteria:
- Able to attend 3 visits at the designated times.
Exclusion Criteria:
- Pregnant
- Known allergy to broccoli, lidocaine, or epinephrine
We found this trial at
1
site
1800 Orleans St.
Baltimore, Maryland 21287
Baltimore, Maryland 21287
410-955-5000
Principal Investigator: Bernard Cohen, MD
Phone: 410-955-5933
Johns Hopkins Hospital Patients are the focus of everything we do at The Johns Hopkins...
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