Mitochondrial Dysfunction, Inflammation, and White Matter Integrity in Youth With Mood Disorders

This is a research study looking at the biology of mood disorders in youth. The investigator
ask participants to come in for three visits. The first visit includes a diagnostic
interview, some questionnaires, and a blood draw. The blood draw will take place at the
Fairview Outpatient Lab. A trained technician will take about 4 teaspoons of blood from the
participant's arm. This sample will be sent to Dr. Andreazza in Toronto, Ontario for further
analysis. No results will be shared with the participant.

The second visit would take place at the Fairview Riverside Ambulatory Research Center (ARC).
This appointment will include the Trier Social Stress Test (TSST). For this, participants
will be asked to prepare a short speech for 5 minutes and deliver that speech for in front of
an audience with another task to follow. This task will be videotaped. After the participant
has completed the speech task, the participant will be asked to complete a number of laptop
games which will test for things like attention and memory. Participants will be asked to
provide 5 saliva samples throughout the course of this visit so the investigators can look at
Cortisol which is a hormone naturally found in saliva. The investigators will also ask
participants to complete an additional set of 5 spit samples at home during the course of a
normal day and bring those back to their next appointment.

The third visit would take place at the Center for Magnetic Resonance Research (CMRR) and
would include a brain MRI. The MRI scan is noninvasive and takes about 2 hours. Participants
will be asked to provide a urine sample and complete a urine toxicology screen (drug test)
and pregnancy test (if female) prior to the MRI scan. Participants may not participate if
drug or pregnancy testing is positive. The MRI involves taking pictures of the brain, from
which measures of the integrity of certain brain tissues can be derived. For the scan,
participants will be asked to lie down quietly on a scanner bed. Once they are inside the
scanner, it will start to take pictures. While in the scanner, participants will be doing
things like resting, or listening to music.

Inclusion Criteria - All Participants:

- Age 13-18 years old

- Both child and guardian are English speaking

Inclusion Criteria - Patient Group:

- Has a diagnosis of a depressive disorder, a bipolar spectrum disorder (type 1, type 2,
Unspecified), or an unspecified mood disorder based on the Diagnostic Statsitical
Manual 5 (DSM-5)

Inclusion Criteria - Healthy Controls

- Does not meet criteria for any psychiatric disorders based on the DSM-5

- Does not have a family history of psychotic or mood disorders in first degree relative

Exclusion Criteria:

- Past history of brain damage, a seizure disorder, increased intracranial pressure,
history of head trauma with loss of consciousness for >15 minutes, history of stroke,
mental retardation, or other serious neurological disorder

- MRI contraindications such as braces, metal implants, implanted medical devices, or
claustrophobia

- Intelligence quotient < 80

- Severe or acute medical illness

- A history of current (past 3 months) substance disorder (defined by DSM-5) with the
exception of Nicotine

- Refusal to cooperate with study procedures

- Active suicidal ideation with intent

- Self-injury that requires medical attention (e.g. stitches) or gets significantly
worse during the course of the study