The Cholesterol Lowering Effects of Strawberry

The trial is designed as a randomized, 2-arm, 2-sequence, within-subject, cross-over, chronic
feeding study. The arms of the study will include regular consumption of a strawberry or
strawberry-placebo powder equivalent to ~3.5 servings of strawberries/day (50g) freeze-dried
strawberry powder (provided twice a day, 25 grams each time >6h apart).

A planned sample size of 50 men and women will be enrolled into the study. This study will
require one initial screening visit, 4 study visits, and 6 weekly visits. This study will
take approximately 12 weeks per subject to complete.

The initial screening visit will provide subject with the informed consent document and
determine subject eligibility through anthropometric measurements, vital signs, fasting blood
glucose test (finger prick), fasting blood draw, and completion of a survey relate to general
eating, health, and exercise habits.

If willing and eligible to participate, a 3-day food record (2 weekdays and 1 weekend day)
will be instructed at the Screening Visit and collected before study day 1 to assess
subjects' baseline dietary intake pattern. Subjects will be instructed to follow a strictly
limited polyphenolic diet for 7 days prior to the study and throughout their participation
time, while maintaining their usual diet pattern and physical activity, with counseling by
staff investigator and/or Registered Dietitian. A dinner meal will be provided the day before
the study visit to control the second meal effect from the food and beverage intake of the
night before the study visit.

Subject will arrive at the center in a fasted state for at least 10 hours, well hydrated and
rested. Each study visit will require blood draws throughout the visit. After evaluation of
subject's health status (via anthropometric, vital sign and blood glucose measurements and
in-person interview), a Licensed health care professional will take a fasting blood draw with
a butterfly needle. Subjects will be randomized to receive a placebo or treatment beverage
based on randomized treatment sequences at 4 study visits. The sequences of receiving the
beverage treatments at each visit will be randomly assigned to one of two of following
sequences: placebo- treatment beverage or treatment beverage -placebo.

Each study visit will involve blood sample collection at time points 0h (fasting) for
assessment of change in plasma lipid and lipoprotein markers. Each study visit will involve a
2 flow mediated dilatation procedure to assess endothelial function.

Inclusion Criteria:

- Men and post-menopausal women (without menses for 1 year) with moderate
hypercholesterolemia as defined by LDL cholesterol between 115mg/dl-165mg/dl and total
cholesterol >160mg/dl preferred but not to exceed 240mg/dl

- 40-69 years of age

- Body Mass Index (BMI) range from 25 to 38.9 kg/m2

- Nonsmoker

- No clinical evidence of cardiovascular, metabolic, respiratory, renal,
gastrointestinal or hepatic disease

- Not taking any medications that would interfere with outcomes of the study, i.e. lipid
lowering medications, anti-inflammatory drugs, dietary supplements, etc…

- Able to provide informed consent

- Able to comply and perform the procedures requested by the protocol

- Weight stable: not gained or lost weight +/- 5 kg in previous 3 months

Exclusion Criteria:

- Men and women who smoke

- Past smokers: abstinence for minimum 2 years

- Men and women with known or suspected food intolerance, allergies or hypersensitivity

- Men and women known to have/diagnosed with diabetes mellitus

- Men and women who have fasting blood glucose concentrations > 110mg/dL

- Men and women who have uncontrolled blood pressure >120 mmHg/80 mmHg

- Men and women with documented vascular disease, e.g., heart failure, myocardial
infarction, stroke, angina, related surgeries, etc...

- Men and women with cancer other than non-melanoma skin cancer in previous 5 years

- Women who are known to be pregnant or who are intending to become pregnant over the
course of the study

- Women who are breast-feeding

- Taking medication or dietary supplements that may interfere with the outcomes of the
study; e.g., antioxidant supplement, anti-inflammation, lipid lowering medication,
blood pressure lowering medication, etc... Subjects may choose to go off dietary
supplements (requires 30 days washout); e.g., fish oil, probiotics, etc...

- Men and women who have donated blood within 3 months of the Screening Visit and blood
donors/participants for whom participation in this study will result in having donated
more than 1500 milliliters of blood in the previous 12 months.

- Men and women who are vegans

- Substance (alcohol or drug) abuse within the last 2 years

- Excessive coffee and tea consumers (> 4 cups/d) and polyphenol consumers (>2 cups
fresh/day

- Men and women who do excessive exercise regularly or athlete

- Unstable weight: gained or lost weight +/- 5 kg in previous 3 months

- Women who are taking unstable dose and brand of hormonal contraceptives and/or stable
dose and brand less than 6 months.