fPAM for the in Vivo Depth Measurement of Pigmented Lesions and Melanoma Depth

The investigators propose the use of functional photoacoustic microscopy (fPAM) to evaluate
both benign and malignant pigmented lesions for tumor depth. Through fPAM analysis followed
by histological examination, the investigators anticipate that they will be able to
non-invasively determine tumor depth of pigmented lesions (moles and melanoma). In melanoma,
tumor depth (Breslow's depth) is not only an important prognostic indicator, but also directs
surgical treatment. The ultimate goal is to develop a sensitive clinical tool that will allow
non-surgical evaluation of pigmented lesions, which eventually, will aid in melanoma
diagnosis and management - potentially an earlier and more definitive surgical management.

In addition, the investigators propose to use the combination of fPAM and single-cell PAM to
respectively image CTCs in trunk vessels and cuticle capillaries. Based on the investigators'
murine models, the investigators anticipate that they will be able to differentiate CTCs from
other blood cells and reliably calculate CTC concentration in a non-invasive manner. CTC
concentration has been demonstrated to be a valuable indicator of a melanoma's metastatic
potential and a potential tool in evaluating treatment efficacy. The ultimate goal is to
develop a sensitive imaging device that will allow accurate evaluation of the risk of
melanoma recurrence and metastases, that may facilitate treatment monitoring.