DNA Analysis of Blood and Tissue From Patients With Lung Cancer

OBJECTIVES:

- Assess the role of genetic polymorphisms in lung-cancer risk in patients with lung
cancer.

- Assess the role of germline polymorphisms in DNA repair genes, p53 pathway genes, and a
matrix metalloproteinase gene (tumor invasion factor) in lung-cancer risk, after
adjusting for potential confounders.

- Assess the roles of gender and age in the genetic susceptibility of lung cancer.

- Assess the role of genetic polymorphisms in modifying the diet-lung cancer risk
association (gene-diet and exploratory gene-gender-diet and gene-gene-diet
interactions).

- Assess the role of polymorphisms on the risk of developing specific histologic subtypes
of lung cancer in case-only analyses.

- Determine whether continued exposures to mainstream and/or second-hand tobacco smoking
is associated with poorer clinical prognosis in patients treated for lung cancer.

OUTLINE: This is a case-control study.

Blood samples are collected from patients and controls. Samples are analyzed by polymerase
chain reaction for gene polymorphisms. Genes to be studied include GSTP1, GSTM1, GSTT1,
ERCC2, XRCC1, EPHX, NAT-2, p53 gene, CYP1A1, NQO1, MnSOD, and GPX1. Tissue samples collected
from patients undergoing surgery and blood samples are archived for future studies.

Patients and controls complete questionnaires about diet, medical history, and
occupational/environmental history.

PROJECTED ACCRUAL: A total of 3,400 patients and 3,400 controls will be accrued for this
study.

DISEASE CHARACTERISTICS:

- Patient:

- Newly diagnosed lung cancer

- Patient at Massachusetts General Hospital (MGH) or Boston Medical Center
Cancer Center

- Control:

- Nonblood-related friend or spouse of the patient

- No specific matching characteristics

- Friend or spouse of other hospital patients

PATIENT CHARACTERISTICS:

- Not specified

PRIOR CONCURRENT THERAPY:

- Not specified

EXCLUSIONS:

Healthy controls with other primary cancers (melanoma, colon, etc.) are excluded Vulnerable
populations are excluded