Vigil™ + Nivolumab in Advanced Non-Small Cell Lung Cancer

This is an open label phase 2 study to evaluate the combination of Vigil™ autologous tumor
cell immunotherapy and nivolumab PD-1 inhibitor therapy in patients with advanced or
metastatic NSCLC that is progressive on or after one prior platinum-based systemic therapy
(ALK or EGFR mutation-targeted therapy should have been received if appropriate mutation
present).

Patients undergoing a standard surgical procedure (e.g., tumor biopsy, palliative resection,
or thoracentesis of malignant pleural effusion) may elect to have tumor tissue procured for
manufacture of Vigil™ vaccine. Patients meeting study eligibility criteria will receive
doublet therapy comprising of (i) Vigil™ 1 x 10^7 cells by intradermal injection every 2
weeks for a minimum of 4 and a maximum of 12 doses and (ii) nivolumab 3 mg/kg by intravenous
infusion over 60 minutes every 2 weeks. Three to six weeks after tissue procurement has
occurred eligibility will be reconfirmed by the study site. Subjects must begin the study
regimen within 6 weeks of tissue procurement. Radiological assessment of tumor response will
be performed at screening, Cycle 5 (Week 9) and approximately every 2 months thereafter
until progressive disease unless the subject is lost to follow-up, withdraws consent for
study related procedures, or initiates another cancer therapy. Tumor biopsy for correlative
studies should be obtained at tissue procurement and at Cycle 5 (Week 9). Peripheral blood
mononuclear cells (PBMC) for correlative studies should be obtained before tumor
procurement, and prior to initiation of study therapy on Day 1 at Cycle 1 (Week 1), Cycle 5
(Week 9), Cycle 9 (Week 17) and EOT.

Tissue Procurement Inclusion Criteria:

Patients will be eligible for tissue procurement for the Vigil™ manufacturing process, if
they meet all of the following criteria:

1. Histologically or cytologically confirmed diagnosis of NSCLC.

2. Age ≥ 18 years.

3. Locally advanced or metastatic disease that is progressive after one prior
platinum-based systemic chemotherapy regimen

1. Adjuvant therapy will count as a line of therapy if administered within 6 months
of relapse).

2. Subjects with EGFR or ALK mutations should also have received appropriate
targeted therapy.

4. No systemic therapy, immunologic therapy or investigational therapy within 3 weeks
and no radiation therapy within 1 week prior to tumor procurement for vaccine
manufacture.

5. Planned standard of care surgical procedure (e.g., tumor biopsy or palliative
resection or thoracentesis) and expected availability of a cumulative mass of ~10-30
grams tissue ("golf-ball" size) or pleural fluid estimated volume ≥ 500mL (must be
primary tap) for immunotherapy manufacture.

6. At least one area of cancer, not intended for vaccine manufacture, that is
measureable by RECIST 1.1 criteria.

7. At least one tumor, not intended for vaccine manufacture, that is considered
appropriate for on-treatment biopsy. The same tumor may suffice for both on-treatment
biopsy and RECIST 1.1 measurement so long as imaging occurs prior to biopsy.

8. ECOG Performance Status ≤ 1

9. Estimated survival ≥ 6 months.

10. Ability to understand and the willingness to sign a written informed consent document
for tissue harvest.

Tissue Procurement Exclusion Criteria:

Patients meeting any of the following criteria are not eligible for tissue procurement for
the Vigil™ manufacturing:

1. Any localized anticancer therapy (e.g., radiation, radiofrequency ablation,
cryotherapy) to tumor intended for vaccine manufacture unless unequivocal evidence of
post-treatment disease progression of the target tumor.

2. Medical condition requiring any form of chronic systemic immunosuppressive therapy
(steroid or other) except physiologic replacement doses of hydrocortisone or
equivalent (no more than 30 mg hydrocortisone or 10 mg prednisone equivalent daily
for < 30 days duration

3. Known history of other malignancy unless having undergone curative intent therapy
without evidence of that disease for ≥ 3 years except cutaneous squamous cell and
basal cell skin cancer, superficial bladder cancer, in situ cervical cancer or other
in situ cancers are allowed if definitively resected.

4. Brain metastases unless treated with curative intent (gamma knife or surgical
resection) and without evidence of progression for ≥ 4 months.

5. Any documented history of autoimmune disease with exception of Type 1 diabetes on
stable insulin regimen, hypothyroidism on stable dose of replacement thyroid
medication, vitiligo, or asthma not requiring systemic steroids.

6. Known history of allergies or sensitivities to gentamicin.

7. History of or current evidence of any condition (including medical, psychiatric or
substance abuse disorder), therapy, or laboratory abnormality that might confound the
results of the study, interfere with the patient's participation for the full
duration of the study, or is not in the best interest of the patient to participate,
in the opinion of the treating Investigator.

8. Known HIV or chronic Hepatitis B or C infection.

9. History of pneumonitis or interstitial lung disease.

Study Enrollment Inclusion Criteria:

Patients will be eligible for registration into the trial if they meet all of the
following inclusion criteria:

1. Successful manufacturing of at least 4 vials of Vigil™.

2. ECOG Performance Status ≤ 1

3. Estimated survival ≥ 4 months.

4. Disease that is measurable by RECIST 1.1 criteria.

5. Adequate organ function as defined by the following laboratory values:

Absolute granulocyte count ≥ 1,500/mm3 Absolute lymphocyte count ≥ 500/mm3 Platelets
≥ 75,000/mm3 Hemoglobin ≥ 9 g/dL Creatinine ≤ 1.5x institutional upper limit of
normal Total bilirubin ≤ 1.5x institutional upper limit of normal AST(SGOT) and
ALT(SGPT) ≤2x institutional upper limit of normal or

≤5x institutional upper limit of normal if liver metastases INR / PT and aPTT ≤ 1.5 x
ULN (if not using anticoagulants) Immunological Thyroid Stimulating Hormone within
institutional limits

6. Subject has recovered to CTCAE Grade 1 or better from all adverse events associated
with prior therapy or surgery. Pre-existing motor or sensory neurologic pathology or
symptoms must be recovered to CTCAE Grade 2 or better.

7. If female of childbearing potential, has a negative urine or serum pregnancy test. If
the urine test is positive or cannot be confirmed as negative, a negative serum test
will be required for study entry.

8. Investigator deems the patient to have a tumor appropriate for on-treatment biopsy
and patient agrees to provide tissue biopsy at Cycle 5 (Week 9) for correlative
studies.

9. Ability to understand and the willingness to sign a written informed protocol
specific consent.

Study Enrollment Exclusion Criteria:

In addition to the procurement exclusion criteria, patients will NOT be eligible for study
registration and enrollment if meeting any of the following criteria:

1. Any anti-neoplastic therapy between tissue procurement for vaccine manufacture and
start of study therapy.

2. Live vaccine used for the prevention of infectious disease administered < 30 days
prior to the start of study therapy.

3. Post-surgery complication that in the opinion of the treating investigator would
interfere with the patient's study participation or make it not in the best interest
of the patient to participate.