Evaluation of Intracoronary Hyperoxemic Oxygen Therapy in Anterior Acute Myocardial Infarction Patients (IC-HOT)

A Multi-Center, Consecutively Enrolled Single-Arm Study to confirm the safety and
effectiveness of the delivery of supersaturated oxygen (SSO2) Therapy for 60 minutes
selectively into the left main coronary artery (LMCA) with a commercially available qualified
SSO2 delivery catheter used with the TherOx® DownStream® System and Cartridge in the
treatment of qualified patients presenting with anterior acute myocardial infarction in whom
reperfusion with PCI is successful within six hours after symptom onset.

GENERAL INCLUSION CRITERIA: Candidates for this study must meet ALL of the following
criteria:

Pre-PCI:

1. The subject must be ≥18 and ≤80 years of age.

2. AMI must be anterior (ST-segment elevation >1 mm in two or more contiguous leads
between V1 and V4 or new left bundle branch block).

3. Subject is experiencing clinical symptoms consistent with anterior AMI of ≤6 hour
duration from time of symptom onset until admission to the emergency room.

4. The subject or legally authorized representative has been informed of the nature of
the study, agrees to its provisions and has been provided and signed written informed
consent, approved by the appropriate Institutional Review Board (IRB).

5. Subject and his/her physician agree to all required follow-up procedures and visits.

ANGIOGRAPHIC INCLUSION CRITERIA: These are evaluated after the subject has provided
signed informed consent but prior to enrollment:

6. Based on coronary anatomy, PCI is indicated for revascularization of the culprit
lesion(s) with use of a commercially available coronary stent (bare metal or
drug-eluting, at operator discretion) in the LAD.

7. The primary stented infarct-related lesion(s) must be in the proximal and/or mid-LAD
coronary artery (other lesions in the LAD target vessel, including diagonal branches,
may be treated if clinically indicated).

8. Baseline (pre-PCI) TIMI flow grade 0, 1, 2, or 3 flow in the LAD.

9. Successful angioplasty as documented by <50% diameter residual angiographic stenosis
within all treated culprit lesions with TIMI 2 or 3 flow and no major complications
such as perforation or shock.

10. Expected ability to place the SSO2 delivery catheter in the coronary ostium of the
left main coronary system to deliver SSO2 Therapy with stable, coaxial alignment.

GENERAL EXCLUSION CRITERIA:

Pre-PCI:

1. Prior CABG surgery.

2. Prior myocardial infarction, or known prior systolic dysfunction (known ejection
fraction <40% by any prior measure or regional wall motion abnormalities; this
criterion does not include left ventricular dysfunction induced by the acute MI).

3. Thrombolytic therapy administered for this STEMI.

4. An elective surgical procedure is planned that would necessitate interruption of
anti-platelet agents during the first 30 days post-enrollment.

5. Subjects who previously underwent coronary stent implantation and in whom coronary
angiography demonstrates stent thrombosis to be the cause of the anterior AMI.

6. Subjects who have previously undergone an angioplasty or stenting procedure in the
left anterior descending coronary artery.

7. Subjects with ventricular pseudoaneurysm, VSD, or severe mitral valve regurgitation
(with or without papillary muscle rupture).

8. Any contraindication to MRI imaging. This will include any of the following
exclusions:

- Cardiac pacemaker or implantable defibrillator;

- Non-MRI compatible aneurysm clip;

- Neural Stimulator (i.e., TENS unit);

- Any implanted or magnetically activated device (insulin pump);

- Any type of non-MRI compatible ear implant;

- Metal shavings in the orbits;

- Any metallic foreign body, shrapnel, or bullet in a location which the physician
feels would present a risk to the subject;

- Any history indicating contraindication to MRI, including claustrophobia or
allergy to gadolinium;

- Inability to follow breath hold instructions or to maintain a breath hold for >15
seconds; and

- Known hypersensitivity or contraindication to gadolinium contrast.

9. Known impaired renal function (creatinine clearance <30 ml/min/1.73 m2

10. Known platelet count <100,000 cells/mm by the MDRD formula) or on dialysis. 3 or
>700,000 cells/mm3

11. Subject has active bleeding or a history of bleeding diathesis or coagulopathy
(including heparin induced thrombocytopenia), or refusal to receive blood transfusions
if necessary. or a known Hgb <10 g/dL.

12. History of intracerebral mass, aneurysm, arteriovenous malformation, or hemorrhagic
stroke.

13. Stroke or transient ischemic attack within the past six (6) months, or any permanent
neurological defect.

14. Gastrointestinal or genitourinary bleeding within the last two (2) months, or any
major surgery (including CABG) within six weeks of enrollment.

15. Subject has received any organ transplant or is on a waiting list for any organ
transplant.

16. Subject has other medical illness (e.g., cancer, dementia) or known history of
substance abuse (alcohol, cocaine, heroin, etc.) that may cause non-compliance with
the protocol, confound the data interpretation, or is associated with limited life
expectancy of less than one year.

17. Subject has a known hypersensitivity or contraindication to unfractionated heparin,
abciximab, aspirin, bivalirudin, cangrelor, clopidogrel, ticlopidine, prasugrel, or
ticagrelor that cannot be adequately premeditated.

18. Current use of warfarin, dabigatran, or factor Xa inhibitors, or known intent to
administer these agents after the primary PCI.

19. Subjects presenting with or developing in the cath lab prior to completion of the
primary PCI procedure any of the following conditions: cardiogenic shock (SBP <80 mmHg
for >30 minutes), or requiring IV pressors or emergent placement of an intra-aortic
balloon pump (IABP), Impella, or other hemodynamic support for hypotension treatment,
or cardiopulmonary resuscitation for >10 minutes, or ventricular fibrillation or
tachycardia requiring cardioversion or defibrillation.

20. Severe known cardiac valvular stenosis or insufficiency, pericardial disease, or
non-ischemic cardiomyopathy.

21. Any significant medical or social condition which in the investigator's opinion may
interfere with the subject's participation in the study or ability to comply with
follow-up procedures, including MRI (e.g. alcoholism, dementia, lives far from the
research center, etc.).

22. Current participation in other investigational device or drug trials that have not
finished the primary endpoint follow-up period.

23. Previous enrollment in this study.

ANGIOGRAPHIC EXCLUSION CRITERIA: These are evaluated after the subject has provided
signed Informed Consent but prior to enrollment:

24. Anticipated inability to achieve a stable coaxial position in the left main coronary
artery with the SSO2 delivery catheter.

25. Treatment during the index procedure of any lesion in either the left main, LCX
(including the ramus), and/or RCA.

26. Post-index procedure planned intervention within 30 days (i.e., PCI of non-target
lesions in any vessel, or CABG). Note: Planned revascularization (PCI or bypass) of a
non-target lesion >30 days following the index procedure is allowed.

27. Anterior MI is due to thrombosis within or adjacent to a previously implanted stent.

28. Left ventriculography demonstrates severe mitral regurgitation, a ventricular septal
defect, or a pseudoaneurysm.

29. Any left main coronary artery stenosis >20%.

30. Any untreated LAD or diagonal branch lesion is present with diameter stenosis > 50% in
a vessel with reference vessel diameter > 2.0 mm (visually estimated), or for which
PCI will be required before the MRI study.

31. Presence of a non-stented coronary dissection with NHLBI grade >B upon completion of
the PCI procedure.