Brentuximab Vedotin in Relapsed/Refractory Germ Cell Tumors

Primary Objective To determine the anti-tumor efficacy of brentuximab vedotin in relapsed/
refractory NSGCT.

Secondary Objectives

1. To determine the progression free survival in patients with relapsed/ refractory NSGCT
treated with brentuximab vedotin.

2. To determine the overall survival of patients with relapsed/ refractory NSGCT treated
with brentuximab vedotin.

3. To determine the safety and tolerability of brentuximab vedotin in this patient
population.

Eligible patients will be divided into two cohorts, those who are CD30 positive and those who
are CD30 negative/unknown. Both groups will be treated similarly and in parallel but analyzed
separately. CD30 status may be unknown in the unlikely case of tumor-marker-only relapse or
when a fresh tumor biopsy is not feasible, and archival tumor tissue is not obtainable
despite efforts to do so. These patients will be included in the CD30 negative cohort for
analysis purposes, since statistically NSGCT are more likely to be CD30 negative. The number
of such patients with unknown CD30 status should not exceed 5 patients.

Eligible patients will be treated with brentuximab vedotin at 1.8 mg/kg IV every 3 weeks
(maximum dose of 180 mg) indefinitely until disease progression, unacceptable toxicity, or
study closure.Eligible patients with grade 2 peripheral neuropathy at enrollment will be
treated with brentuximab vedotin at 1.2 mg/kg IV every 3 weeks (maximum dose of 180 mg)
indefinitely until disease progression, unacceptable toxicity, or study closure. Response to
treatment will be assessed clinically with history, physical exam and tumor markers
measurement (BHCG and AFP) on day 1 of each cycle and with CT scans after cycle 2, 4, and
every 4 cycles thereafter while receiving treatment.

Inclusion Criteria

Subject must meet all of the following applicable inclusion criteria to participate in this
study:

1. Age ≥ 18 years at the time of informed consent.

2. Patients with histologically or serologically confirmed relapsed/refractory
non-seminoma germ cell tumor, (i.e., embryonal carcinoma, choriocarcinoma, or yolk sac
tumors) including female GCT and primary mediastinal NSGCT.

3. Patients must have progressed after prior high dose chemotherapy (HDCT) treatment,
been deemed not to be a candidate for high dose chemotherapy or refused high-dose
chemotherapy, and be considered incurable by other standard therapies including
further chemotherapy or surgery. There is no maximum allowable number of previous
therapies.

"Failure" of prior therapy is defined as:

1. A >25% increase in the products of perpendicular diameters of measurable tumor
masses during prior therapy which are not amenable to surgical resection.

2. The presence of new tumors which are not amenable to surgical resection.

3. An increase in AFP or beta-hCG (two separate determinations at least one week
apart are required if rising tumor markers are the only evidence of failure).

NOTE: Patients with clinically growing "teratoma" (normal declining tumor markers and
radiographic or clinical progression) should be considered for surgery.

4. Patients must have evidence of recurrent or metastatic carcinoma by one or more of the
following:

i) The appearance of metastatic disease by standard imaging techniques ii) The
appearance of rising serum tumor marker, AFP or beta-hCG NOTE: If a rising tumor
marker is the only evidence of progressive disease, at least 2 consecutive rising
values at least one week apart are needed. Patients with only evidence of disease is
rising tumor marker AFP and beta-hCG will be provided alternate causes of increased
serum levels of these markers are not present, such as cross reaction with luteinizing
Hormone (LH) (that can be tested if needed by testosterone suppression of LH),
hepatitis, use of marijuana or second primary tumor, etc.

5. Patients with primary medistinal non seminomatous germ cell tumor are eligible if they
have received first line platinum based chemotherapy and their recurrence is not
amenable to surgical resection based on the treating physician expert opinion.

6. Patients with late relapse (>2 years) of non seminomatous germ cell tumors are
eligible if they have received first line platinum based chemotherapy and their
recurrence is not amenable to surgical resection based on the treating physician
expert opinion.

7. Patients with brain metastases are allowed onto the study as long as patients have
completed their treatment for brain metastasis, no longer require corticosteroids, and
are asymptomatic. Subjects with neurological symptoms should undergo a head CT scan or
brain MRI to exclude brain metastasis, at the discretion of the treating physician.

8. Patients with ECOG performance status of 0-2.

9. Adequate organ and marrow function as defined below:

1. Hemoglobin ≥ 8 g/dL

2. Absolute neutrophil count ≥ 1,000/mm3

3. Platelet count ≥ 75,000/mm3

4. Total bilirubin ≤ 1.5 × ULN except patients with documented Gilbert's syndrome (≤
3 × ULN)

5. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ≤ 2.5 × ULN;
for patients with hepatic metastases, ALT and AST ≤ 5 × ULN

6. Calculated creatinine clearance ≥ 30 mL/min as determined by the Cockcroft-Gault
equation.

10. Patients who are willing and able to comply with the protocol and study procedures
including willingness to undergo tumor biopsy for tumor cells before therapy to assess
for CD30 status (unless archival tumor tissue from orchiectomy or other previous
sample is not obtainable despite efforts to do so and a fresh tumor biopsy is not
feasible).

11. Females of childbearing potential must not be pregnant or breast-feeding. Male and
female patients of reproductive potential must agree to use two forms of highly
effective contraception from the screening visit through 28 days after the last dose
of study drug. Acceptable forms of effective contraception include:

- Oral, injected or implanted hormonal methods of contraception.

- Placement of an intrauterine device (IUD) or intrauterine system (IUS).

- Barrier methods of contraception: Condom or Occlusive cap (diaphragm or
cervical/vault caps) with spermicidal foam/gel/film/cream/suppository.

- Male sterilization (with the appropriate post-vasectomy documentation of the
absence of sperm in the ejaculate).

- True abstinence: When this is in line with the preferred and usual lifestyle of
the subject. [Periodic abstinence (e.g., calendar, ovulation, symptothermal,
post-ovulation methods) and withdrawal are not acceptable methods of
contraception.] Pregnancy tests for females of childbearing potential are
required; must be serum at screening and the post treatment safety assessment
visit. A positive urine pregnancy test must be confirmed by a serum pregnancy
test and a pelvic US since some NSGCT may secrete beta-hCG and cause a false
positive pregnancy. A pelvic US does not need to be repeated with each cycle
unless the treating physician thinks it is necessary to do so.

12. Potential subject must have the ability to understand (as judged by the treating
physician) and willingness to provide written informed consent and HIPAA authorization
for release of personal health information.

NOTE: HIPAA authorization may be included in the informed consent or obtained separately.
Written informed consent must be obtained from a potential subject prior to the conduct of
any study-specific procedures.

Exclusion Criteria

Subjects meeting any of the criteria below may not participate in the study:

1. Patients with pure seminoma.

2. Patients with pure teratoma.

3. Chemotherapy within 2 weeks of initiating study treatment. There is no maximum
allowable number of previous therapies.

4. Major surgery within 3 weeks of starting study treatment. There is no minimum time
requirement for minor procedures such as biopsy or vascular access placement.

5. Radiation within 2 weeks of starting study treatment.

6. ≥ Grade 3 neuropathy at the time of enrollment.

7. Pregnancy or breast-feeding.

8. Previous treatment with any anti-CD30 directed therapy.