Evaluation of Jarro-Dophilus EPS® Probiotic Formulations

Participants will be screened using the International Physical Activity Questionnaire
(questions regarding physical activity, including intensity, duration and frequency) and
inclusion/exclusion criteria. Participants will be scheduled to begin a 7-day baseline
period, during which participants will complete daily questionnaires of bowel movement
frequency, gastrointestinal symptoms and wellness. On or about day 8, height and weight will
be determined. Participants will then be randomized to one of the following groups:

Group 1: Jarro-Dophilus EPS® (5 billion CFU/capsule), Group 2: Jarro-Dophilus EPS® Higher
Potency (25 billion CFU/capsule), Group 3: Placebo capsule

Study participants will continue to complete the daily online questionnaire throughout the 28
days of treatment and for 7 days post treatment. Participants will also be asked to complete
a weekly questionnaire on gastrointestinal symptoms (Gastrointestinal Symptom Rating Scale)
throughout the entire study. In addition, participants in each group will be asked to collect
one stool sample at baseline, one stool sample during week 4 of treatment and an additional
sample during the washout period. Stool samples will be analyzed and quantified for the
probiotic bacteria and changes in the microbiota.

Participants will be asked to return any unconsumed supplements at the end of the treatment
period. Additionally, the participants will be weighed after the treatment period and the
washout period.

Inclusion Criteria:

To participate in the study you must

- be between18-50 years of age

- be willing to have your height and weight measured and provide demographic information

- be willing to consume a probiotic or placebo capsule daily for 4 weeks

- be willing to provide 3 stool samples during the study

- be willing to complete a daily questionnaires regarding general and gastrointestinal
wellness and the Gastrointestinal Symptom Rating Scale weekly throughout the entire
6-wk study

- have daily access to a computer with Internet access for the entire 6-wk study

- be willing and able to provide a valid social security for study payment purposes

- be willing and able to provide a valid social security for study payment purposes

- be willing to complete the International Physical Activity Questionnaire (asks you to
report physical activity including intensity, duration and frequency)

Exclusion Criteria:

To participate in the study you must NOT

- be currently taking medications for constipation or diarrhea

- have previously or are you currently being treated for any diseases or illnesses such
as gastrointestinal disease (gastric ulcers, Crohn's, Celiac, ulcerative colitis,
etc.), other chronic diseases (diabetes, kidney disease, etc.) or immune-compromising
diseases or conditions (HIV, AIDS, autoimmune, hepatitis, cancer, transplant patient
etc.)

- have any known allergy to milk, milk protein, soy, gluten or have gluten sensitivity

- have taken antibiotics within the past 4 weeks prior to randomization

- be currently taking a probiotic supplement and are unwilling to discontinue it a
minimum of 2 weeks prior to the study start

- be a current smoker.