Autonomic Manifestations of Testosterone Deficiency in Men

The investigators will recruit 40 men between the ages of 40 to 80-years-old with low
testosterone. Participants will be selected among those males referred to the Texas Tech
University Health Sciences Center's (TTUHSC's) Internal Medicine Endocrinology Division
outpatient clinic for the study and management of testosterone deficiency and to the TTUHSC
Urology Department for diagnosis and management of prostate cancer that will result in
testosterone deficiency due to surgical or pharmacological castration.

After recruitment, patients will have an initial standard clinical visit where aspects such
as fatigue and anxiety will be investigated, followed by a full battery of autonomic testing
(tilt table, heart rate response to deep breathing, Valsalva, and the Quantitative Sudomotor
Axon Reflex Test (QSART).

Those patients with an initial diagnosis of testosterone deficiency will be initiated on
testosterone replacement (intramuscular or skin routes) as standard of care, followed by a
second visit in three (3) months--after normalization of serum testosterone levels--to
evaluate changes in anxiety and fatigue level, and to repeat the cardiovascular autonomic
function test.

Those patients with primary diagnosis of prostate cancer and normal serum testosterone
levels will be evaluated a second time after confirmation of low testosterone, as described
above. Standard questionnaires will be used to evaluate fatigue and anxiety. The autonomic
testing of the heart will include tilt table (tilt the patient 70 degree by special designed
table) and heart rate response to deep breathing, and Valsalva maneuver. Descriptive
statistics will be compared between before and after treatment using different statistical
methods.

Inclusion Criteria for first group:

1. Patients with testosterone deficiency (total testosterone< 277 ng/dL [9.6 nmol/L])
measured between 7-10 am, and with one or more symptoms and signs suggestive of
androgen deficiency as mainly determined by history.

- Reduced sexual desire (libido)

- Decreased spontaneous erections

- Breast discomfort, gynecomastia

- Loss of body (axillary and pubic) hair - reduced shaving

- Very small (<5ml) or shrinking testes

- Height loss

- Low trauma fracture

- Low bone mineral density

- Hot flushes

- Sweats

- Decreased energy

- Decreased motivation

- Decreased initiative

- Decreased self confidence

- Feeling sad or blue

- Depressed mood

- Dysthymia

- Poor concentration

- Poor memory

- Sleep disturbance

- Increased sleepiness

2. Age 40 to 80 years

Inclusion Criteria for second group:

1. Patients who underwent chemical or surgical castration for prostatic cancer who are
going to receive androgen deprivation therapy.

2. Age 40 to 80 years

Exclusion Criteria (both groups):

1. Patients with history or current diagnosis of:

- Atrial fibrillation.

- Cardiac arrythmia

- Pacemaker placement.

- Myocardial infarction < 3 months

- Uncontrolled diabetes mellitus with hemoglobin A1c > 8.5% in the last 6 months.

- Diabetes mellitus with autonomic neuropathy

- Breast cancer

- Heart failure with left ventricular ejection fraction below 35%.

- Severe sleep apnea.

- Recent eye surgery (< 3 months)

- Recent ischemic stroke (< 3 months)

- History of retinal detachment.

- History of brain aneurysm.

- Severe chronic obstructive pulmonary disease (COPD) on oxygen therapy.

- Intraocular hemorrhage and lens dislocation.

- Glaucoma.

- Thyroid disease.

- Not literate in English

Exclusion Criteria for patient who will receive testosterone treatment

- Metastatic prostate cancer

- Prostate-specific antigen (PSA) concentration >4.0 mcg/L,

- Prostate-specific antigen (PSA) >3.0 mcg/L in high-risk men (African-Americans or men
with first-degree relatives with prostate cancer). (Measured at clinic visit 1)

- Polycythemia (hemoglobin level >18 g/dL, hematocrit > 52%) (measured at clinic visit
1)

- Severe acne.

- History of venous or arterial thrombosis.

- Persons not literate in English