Trial to Evaluate Safety and Immunogenicity of an Ebola Zaire Vaccine in Healthy Adults

Ebola Zaire is a filovirus that has caused devastating epidemics of hemorrhagic fever in
South Africa. Research is underway to create a safe and effective vaccine to protect against
Ebola disease, especially for the military and health care workers. Promising animal studies
with this vaccine indicate safety and immunogenicity, and the vaccine platform used to
deliver the Ebola protein antigen has been successful in creating a safe and protective
immune response in people. Note that only one Ebola protein is used in this vaccine; since
the entire intact Ebola virus is required for infection, it is impossible to get Ebola
disease from this vaccine.

The study targets enrollment of 39 healthy adults. These participants are divided into 3
groups that will be administered one of three dose levels of the vaccine (low, medium,
high). The study participants will receive two doses of vaccine: one on day 1 and the second
on day 28 (1 month). Three participants at each dose level will act as controls and receive
a placebo instead of the active vaccine. A total of 13 visits to the clinic are required
over a period of 26 weeks. The total study is expected to take 9 months.

INCLUSION CRITERIA:

Subjects who have provided written informed consent and an authorization for disclosure of
protected health information must meet the following criteria:

1. Healthy adult men or women, 18 to 60 (inclusive) years of age.

2. Have provided written informed consent prior to screening procedures.

3. Free of clinically significant health problems, as determined by pertinent medical
history, physical examination without significant findings in the 28 days prior to
enrollment, and clinical judgment of the Investigator.

4. Agrees not to have, or plan to have, non-study vaccines within 60 days after
receiving the initial study vaccine, unless medically indicated (i.e., tetanus,
rabies vaccine).

5. Agrees not to have contact with ruminant animals or other hoofed animals such as
horses, pigs and cows 7 days after each vaccination.

6. Available, able, and willing to participate for all study visits and procedures
through Day 182 (Week 26).

7. Be willing to minimize blood and body fluid exposure of others for 7 days after
vaccination by:

- using effective barrier prophylaxis, such as latex condoms, during penetrative
sexual intercourse;

- avoiding the sharing of needles, razors, or toothbrushes; and

- avoiding open mouth kissing.

8. Body mass index (BMI) less than 40 kg/m2.

9. Laboratory criteria without clinically significant findings within 28 days prior to
enrollment:

- hemoglobin ≥11.5 g/dL for women and ≥13.5 g/dL for men.

- white blood cell count ≥3500 cells/mm3.

- differential either within institutional normal range or accompanied by site
physician approval;

- platelets within normal limits;

- alanine aminotransferase (ALT) or aspartate aminotransferase (AST) and alkaline
phosphatase within upper limit of normal range or as approved by the
Investigator; and

- serum creatinine within upper limit of normal range or as approved by the
Investigator.

10. Negative for Food and Drug Administration (FDA) approved HIV blood test.

11. Negative hepatitis B surface antigen (HbsAg).

12. Negative antibody to hepatitis C virus (antiHCV).

13. Normal urinalysis defined as negative or trace glucose, protein, and blood
(non-menstruating females) by dipstick.

14. Negative urine pregnancy test for women of childbearing potential.

15. Non-pregnant, non-lactating females must meet one of the following criteria: no
reproductive potential because of menopause (one year without menses) or because of a
hysterectomy, bilateral oophorectomy, or tubal ligation; or subject agrees to be
heterosexually inactive at least 21 days prior to enrollment and throughout the
duration of the study; or agrees to consistently practice contraception at least 21
days prior to enrollment and throughout the duration of the study by one of the
following methods: abstinence, condoms (male or female with or without a spermicide),
diaphragm or cervical cap with spermicide, intrauterine device, contraceptive pills
or patch, Norplant, Depo-Provera or other FDA approved contraceptive method, or male
partner has previously undergone a vasectomy as declared in medical history.

EXCLUSION CRITERIA:

Any subject who meets any of the following criteria will not qualify for entry into the
study:

1. History of prior infection with a filovirus or prior participation in a filovirus
vaccine trial.

2. History of prior infection with VSV or receipt of a VSV vectored vaccine.

3. Has traveled to an area where the World Health Organization (WHO) has declared as an
Ebola outbreak zone.

4. Healthcare worker who has direct contact with patients (nurse, physician, dentist,
emergency medical technician, dental hygienist).

5. Has a household contact (HHC) who is immunodeficient, on immunosuppressive
medications, HIV positive, pregnant or breast-feeding, or has an unstable medical
condition.

6. Breast-feeding, or is a childcare worker, or HHC, who has direct contact with
children, 5 years of age or younger.

7. Direct hands-on job preparing food in the food industry.

8. History of employment in an industry involved in contact with ruminant animals, other
hoofed animals such as pigs and horses, veterinary sciences, or other potential
exposure to VSV.

9. History of employment or activity that involves potential contact with filoviruses.

10. History of severe local or systemic reactions to any vaccination or a history of
severe allergic reactions.

11. Known allergy to any rVSVN4CT1 vectored vaccine component.

12. Receipt of investigational product (IP) up to 30 days prior to randomization or
ongoing participation in another clinical trial except observational studies.

13. Receipt of licensed non-live or live vaccines within 30 days prior to planned study
immunization.

14. Ability to observe possible local reactions at the eligible injection sites (deltoid
region) is, in the opinion of the Investigator, unacceptably obscured due to a
physical condition or permanent body art.

15. Acute or chronic, clinically significant dermatologic, psychiatric, hematologic,
pulmonary, cardiovascular, or hepatic or renal functional abnormality as determined
by the Investigator based on medical history, physical examination, and/or laboratory
screening test. This would include a known autoimmune arthritis, hemoglobinopathy or
coagulation abnormality as judged by the Investigator.

16. Leukopenia due to a clinical or pathological process, unless leukopenia is directly
attributable to a transient process (e.g., acute viral infection) and resolution has
been documented at time of enrollment.

17. Any screening or baseline laboratory test which in the opinion of the Investigator,
is considered clinically significant.

18. Any cytotoxic therapy in the previous 5 years.

19. Diabetes mellitus (type I or II), with the exception of previous gestational
diabetes.

20. Any chronic or active neurologic disorder, such as migraines (including silent),
seizures or epilepsy (excluding a single febrile seizure as a child as judged by the
Investigator).

21. Have a known history of Guillain Barré Syndrome (GBS).

22. Have an active malignancy or history of metastatic or hematologic malignancy.

23. Suspected or known alcohol and/or illicit drug abuse within the past 5 years per the
judgment of the Investigator.

24. Moderate or severe illness and/or fever >100.4 °F within 1 week prior to vaccination
(subjects can be rescheduled).

25. Administration of immunoglobulin G (IgGs) and/or any blood products within the 120
days preceding study entry or planned administration during the study period.

26. History of blood donation within 60 days of enrollment or plans to donate within the
study period.

27. Administration of chronic (defined as more than 14 days) immunosuppressants or other
immune modifying drugs within 6 months of study entry: for corticosteroids, this
includes chronic oral >14 days or intraarticular steroids in the past 6 months
(intranasal and topical are allowed).

28. Major surgery or hospitalization planned during the period of study participation.

29. Research staff or the immediate family of research staff directly involved with the
clinical study.

30. Any other significant finding that in the opinion of the Investigator that would
increase the risk of the individual having an adverse outcome from participating in
this study.