Bone Marrow Transplant Studies for Safe and Effective Treatment of Leukemia
| Status: | Completed |
|---|---|
| Conditions: | Cancer, Blood Cancer, Blood Cancer, Blood Cancer, Orthopedic, Hematology, Hematology, Hematology, Leukemia |
| Therapuetic Areas: | Hematology, Oncology, Orthopedics / Podiatry |
| Healthy: | No |
| Age Range: | 10 - 55 |
| Updated: | 4/6/2019 |
| Start Date: | March 27, 1997 |
| End Date: | August 3, 2017 |
HLA-Matched Peripheral Blood Mobilized Hematopoietic Precursor Cell Transplantation Followed by T Cell Add-Back for Hematological Malignancies
Bone marrow transplants (BMT) are one of the accepted therapies used to treat leukemia.
However, BMT have risks of complications. One potentially life-threatening complication is
known as graft-versus-host disease (GVHD).
The GVHD is a reaction caused by an incompatibility between donor cells and recipient cells.
Antigens found on the recipient s cells are recognized by the donor s transplanted white
blood cell lymphocytes. These lymphocytes begin attacking the recipient s cells and tissues
and may lead to death.
One of the most effective ways to prevent this reaction is to remove the lymphocytes from the
transplanted marrow. Unfortunately, without lymphocytes the recipient s immune system will be
lowered and may result in a relapse of leukemia or an infection.
Researchers have shown they can perform effective BMT by removing the lymphocytes prior to
the transplant and then later adding the lymphocytes back. This technique can reduce the
potential for GVHD and preserve the graft-versus-leukemia (GVL) effect of the transplant.
In this study researchers plan to use peripheral blood with lymphocytes removed rather than
bone marrow. In order to increase the number of progenitor cells, the cells responsible for
correcting the leukemia, donors will receive doses of G-CSF prior to the transplant. G-CSF
(granulocyte colony stimulating factor) is a growth factor that increases the production of
progenitor cells in the donor s blood stream.
The study will be broken into two parts. The first part of the study will attempt to
determine if peripheral blood with lymphocytes removed can prevent GVHD while preserving the
GVL effect of the transplant.
In the second part of the study, patients that received the transplant will have the
lymphocytes added-back on two separate occasions in order reduce the chances of relapse and
infection.
The study is designed to treat up to 55 patients ages 10 to 60 years and follow their
progress for 5 years.
However, BMT have risks of complications. One potentially life-threatening complication is
known as graft-versus-host disease (GVHD).
The GVHD is a reaction caused by an incompatibility between donor cells and recipient cells.
Antigens found on the recipient s cells are recognized by the donor s transplanted white
blood cell lymphocytes. These lymphocytes begin attacking the recipient s cells and tissues
and may lead to death.
One of the most effective ways to prevent this reaction is to remove the lymphocytes from the
transplanted marrow. Unfortunately, without lymphocytes the recipient s immune system will be
lowered and may result in a relapse of leukemia or an infection.
Researchers have shown they can perform effective BMT by removing the lymphocytes prior to
the transplant and then later adding the lymphocytes back. This technique can reduce the
potential for GVHD and preserve the graft-versus-leukemia (GVL) effect of the transplant.
In this study researchers plan to use peripheral blood with lymphocytes removed rather than
bone marrow. In order to increase the number of progenitor cells, the cells responsible for
correcting the leukemia, donors will receive doses of G-CSF prior to the transplant. G-CSF
(granulocyte colony stimulating factor) is a growth factor that increases the production of
progenitor cells in the donor s blood stream.
The study will be broken into two parts. The first part of the study will attempt to
determine if peripheral blood with lymphocytes removed can prevent GVHD while preserving the
GVL effect of the transplant.
In the second part of the study, patients that received the transplant will have the
lymphocytes added-back on two separate occasions in order reduce the chances of relapse and
infection.
The study is designed to treat up to 55 patients ages 10 to 60 years and follow their
progress for 5 years.
One of the most effective ways of preventing lethal graft-versus-host disease (GVHD) after
allogeneic bone marrow transplantation (BMT) for leukemia is to remove T-lymphocytes from the
transplanted marrow. The reduced early mortality from T cell depletion is however offset by
an increased risk of leukemic relapse and infection. We have shown that bone marrow
transplants for leukemia depleted of T cells by elutriation and followed by delayed add-back
of donor T cells reduces GVHD while preserving an immune response to the hematologic
malignancy (the so-called graft-versus-leukemia (GVL) or graft-versus-myeloma effect). The
study highlighted a possible benefit of large doses of marrow progenitor cells on transplant
outcome. GVHD was reduced but not prevented by T cell depletion of the marrow. The first
objective of our BMT studies is to prevent GVHD from the transplant while conserving GVL
reactivity. This is a prerequisite to our second objective of determining the risk of GVHD
and the benefit from GVL from add-back of donor lymphocytes. These studies will provide the
basis for a planned trial adding back donor lymphocytes selected in vitro to confer immunity
against infectious agents and residual leukemia without causing GVHD.
In this study we will evaluate the use of T cell depleted peripheral blood progenitor cells
(PBPC) (instead of bone marrow) to optimize the stem cell and lymphocyte dose. Donors will be
given G-CSF and their mobilized PBPC harvested by leukapheresis. To minimize acute GVHD, the
transplant will be T cell depleted, using a new technique developed in normal volunteers
which improves T cell depletion and reduces stem cell loss (protocol 96-H-0049). The study
has two phases: The first phase evaluates engraftment and GVHD following T cell depleted PBPC
transplants. Stopping rules will be used to make modifications to the protocol in the event
of graft failure. Cyclosporine will be withdrawn from the protocol if the incidence of acute
GVHD is low or absent. In the second phase patients will receive add-back of donor
lymphocytes on day 45 and day 100 post transplant to prevent relapse and confer donor-immune
function. The risk of acute GVHD following this procedure will be determined. It is planned
to treat up to 55 patients aged between 10 and 60 years. The end points of the study are
graft take; acute and chronic GVHD, leukemic relapse, transplant-related and all causes of
mortality, cytomegalovirus reactivation and leukemia-free survival. Patients will be followed
for 5 years.
allogeneic bone marrow transplantation (BMT) for leukemia is to remove T-lymphocytes from the
transplanted marrow. The reduced early mortality from T cell depletion is however offset by
an increased risk of leukemic relapse and infection. We have shown that bone marrow
transplants for leukemia depleted of T cells by elutriation and followed by delayed add-back
of donor T cells reduces GVHD while preserving an immune response to the hematologic
malignancy (the so-called graft-versus-leukemia (GVL) or graft-versus-myeloma effect). The
study highlighted a possible benefit of large doses of marrow progenitor cells on transplant
outcome. GVHD was reduced but not prevented by T cell depletion of the marrow. The first
objective of our BMT studies is to prevent GVHD from the transplant while conserving GVL
reactivity. This is a prerequisite to our second objective of determining the risk of GVHD
and the benefit from GVL from add-back of donor lymphocytes. These studies will provide the
basis for a planned trial adding back donor lymphocytes selected in vitro to confer immunity
against infectious agents and residual leukemia without causing GVHD.
In this study we will evaluate the use of T cell depleted peripheral blood progenitor cells
(PBPC) (instead of bone marrow) to optimize the stem cell and lymphocyte dose. Donors will be
given G-CSF and their mobilized PBPC harvested by leukapheresis. To minimize acute GVHD, the
transplant will be T cell depleted, using a new technique developed in normal volunteers
which improves T cell depletion and reduces stem cell loss (protocol 96-H-0049). The study
has two phases: The first phase evaluates engraftment and GVHD following T cell depleted PBPC
transplants. Stopping rules will be used to make modifications to the protocol in the event
of graft failure. Cyclosporine will be withdrawn from the protocol if the incidence of acute
GVHD is low or absent. In the second phase patients will receive add-back of donor
lymphocytes on day 45 and day 100 post transplant to prevent relapse and confer donor-immune
function. The risk of acute GVHD following this procedure will be determined. It is planned
to treat up to 55 patients aged between 10 and 60 years. The end points of the study are
graft take; acute and chronic GVHD, leukemic relapse, transplant-related and all causes of
mortality, cytomegalovirus reactivation and leukemia-free survival. Patients will be followed
for 5 years.
- INCLUSION CRITERIA-PATIENT:
- Ages 10 to 55 years.
- Chronic myelogenous leukemia, any of these categories: chronic phase, accelerated
phase of blast transformation.
- Acute lymphoblastic leukemia, any of these categories: Adults (greater than 18 years)
in first remission with high risk features (presenting leukocyte count greater than
100,000 per cu mm, Karyotypes t9;22, t4, t19, t11, biphenotypic leukemia). All second
remissions, primary induction failure, partially responding or untreated relapse.
- Acute myelogenous leukemia (AML): AML in first remission Except AML with good risk
karyotypes: AML M3 (t15;17), AML M4Eo (inv 16), AML t(8;21). All AML in second or
subsequent remission, primary induction failure and resistant relapse.
- Myelodysplastic syndromes, any of these categories: refractory anemia with excess of
blasts, transformation to acute leukemia, chronic myelomonocytic leukemia.
- Multiple myeloma following initial disease control with chemotherapy.
- Chronic lymphocytic leukemia (CLL) and prolymphocytic leukemia, in remission or
partial remission following fludarabine treatment. Richter transformation of CLL.
- No major organ dysfunction precluding transplantation.
- DLCO greater than 65 percent predicted.
- Left ventricular ejection fraction: greater than 40 percent predicted.
- ECOG performance status of 0 or 1.
- Informed consent given. Informed consent from both parents for minors.
- Women of childbearing age with a negative pregnancy test may participate.
EXCLUSION CRITERIA:
- Pregnant.
- Age greater than 55 or less than 10.
- ECOG performance status of 2 or more.
- Severe psychiatric illness. Mental deficiency sufficiently severe as to make
compliance with the BMT treatment unlikely, and making informed consent impossible.
- Major anticipated illness or organ failure incompatible with survival from BMT.
- DLCO less than 65% predicted.
- Left ventricular ejection fraction: less than 40% predicted.
- Serum creatinine greater than 3 mg/dl.
- Serum bilirubin greater than 4 mg/dl.
- Transaminases greater than 3 x upper limit of normal.
- HIV positive.
- History of other malignancies except basal cell or squamous carcinoma of the skin,
positive PAP smear and subsequent negative follow up (patient).
INCLUSION CRITERIA-DONOR:
- HLA 6/6 or 5/6 matched sibling donor.
- Fit to receive G-CSF and give peripheral blood stem cells (normal blood count,
normotensive, no history of stroke).
- Informed consent given.
EXCLUSION CRITERIA - DONOR:
- Pregnant.
- Severe psychiatric illness. Mental deficiency sufficiently severe as to make
compliance with the BMT treatment unlikely, and making informed consent impossible.
- Donor unfit to receive G-CSF and undergo apheresis. (Uncontrolled hypertension,
history of stroke, thrombocytopenia).
- HIV positive.
We found this trial at
1
site
9000 Rockville Pike
Bethesda, Maryland 20892
Bethesda, Maryland 20892
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