Construction and Use of Lung Tumor Microarray for the Analysis of Gene Expression in Lung Cancer

The proposed research intends to construct a set of tissue microarrays containing different
types of lung cancer tissues for the study of genes associated with lung cancer. In this
study, lung tissue arrays will be generated using paraffin embedded archival tissues
containing adjacent normal tissues and tumors from approximately 300 non-small cell lung
cancers and 100 neuroendocrine tumors of the lung. Corded tissues of 0.6 um in diameter will
be taken from each tumor and arrayed onto recipient paraffin blocks to generate a lung tumor
specific microarray. Four-micrometer thickness sections will be cut from the tissue array
and used to survey gene expression status in arrayed tumors using fluorescence in situ
hybridization (FISH, in situ hybridization, and/or immunohistochemistry methods.
Additionally, approximately 20 sections will be cut from each primary tumor block to isolate
genomic DNA for the analysis of genetic changes in the arrayed tumor samples. The goals of
this proposal are 1) to generate a single tissue block containing multiple tumors, 2) allow
rapid and efficient analysis of gene expressions in a large number of samples using a single
tissue section, and 3) to correlate the molecular changes in the tumors with the clinical
and pathological features of the disease. Due to the experimental nature of the research and
the fact that all tumors obtained are archival tissues at AFIP, the investigators will not
report the result of gene expression back to the subject.

To date, all samples needed for the protocol have been reached and there will be no more
patient accrual but survival status of the patients as well as molecular analysis using the
constructed lung tissue microarrays will continue for the foreseeable future.

- INCLUSION CRITERIA

The human tissue microarrays will include existing archival tissues of normal lung, non
small-cell lung cancer, and combined histological types of lung cancer samples. All tissue
samples are already in existence at the Armed Forces Institute of Pathology.

The primary selection criteria will be the type of cancer, the quality of the tumor block,
and the availability of the sample without regard to age, race, ethnic origin, and gender
of the patient.

Only tissue blocks of suitable fixation quality and proper diagnosis will be used to
generate the lung tissue microarrays.