Vaccination With Autologous, Lethally Irradiated Melanoma Cells Engineered by Adenoviral Mediated Gene Transfer to Secrete Granulocyte-Macrophage Stimulating Factor (GMSF)

- The vaccines created from the participants melanoma cells are scheduled to be given to
the participants on days 1, 8, 15, 29 and every two weeks after until the supply of
vaccine has run out. The amount of vaccine is dependent on the total amount of cells
yielded when the tumor is processed and treated. It is hoped that participants will
receive at least six vaccines.

- The vaccines will be administered in two injections that will be placed underneath the
participants skin. The two injections will be given at the same place on the body.

- If tumor sample yields enough cells, participants will also receive an injection of
non-transduced irradiated melanoma cells. The purpose of this is to measure the amount
of reaction of the participants immune system occuring created by the vaccine.

- If either the vaccine site or DTH placement site has shown a reaction, a punch-biopsy
will be taken. This will consist of a small piece of skin tissue removed under local
anesthesia.

- With vaccine #5, participants will receive a second DTH injection. Two days after the
vaccine and DTH injection, punch biopsies will be taken of both sites.

- At week 10 of treatment (or earlier if necessary), participants will undergo a chest,
abdomen and pelvic CT scan. The physician may also have participants undergo a brain MRI
if indicated at this time.

Inclusion Criteria:

- Stage III patients must have: A) Histologically documented melanoma B) Lymphadenopathy
of at least 2cm in greatest diameter by physical exam or CT scan in a region draining
a known or suspected primary melanoma or in transit metastatic disease of at least 2cm
in greatest diameter by physical exam or CT in a region draining a known primary
melanoma C) refused, not eligible, or failed adjuvant therapy with high dose
a-interferon D) must be able to have all measurable disease removed at time of tumor
harvest

- Stage IV patients must have histologically documented metastatic melanoma

- ECOG Performance Status 0 or 1

- Estimated life expectancy of 6 months or greater

- 18 years of age or older

- Signed Informed Consent

- Greater than 4 weeks from any chemotherapy, radiotherapy, immunotherapy, or systemic
glucocorticoid therapy

- Greater than 6 months since bone marrow or peripheral blood stem cell transplant

Exclusion Criteria:

- Uncontrolled active infection

- Pregnancy or nursing mothers

- Evidence of infection with Human Immunodeficiency Virus, Hepatitis B or C