Chemotherapy-Induced Changes to Cognition and DNA in Breast Cancer Survivors

The primary objective of this proposal is to obtain preliminary data regarding the
association between DNA damage and cognitive functioning in breast cancer survivors.
Specifically, we predict that:

1. Breast cancer survivors treated with chemotherapy and hormonal therapy will have higher
levels of DNA damage as measured by the Comet assay as compared to age and education
matched survivors treated with hormonal therapy only and healthy controls.

2. Survivors who meet criteria for cognitive impairment will have higher levels of DNA
damage as compared to cancer survivors who do not meet criteria for cognitive impairment
and healthy controls.

Inclusion Criteria:

- Breast cancer survivors (Stage I-III) 2-6 years post all cancer treatments, but
currently receiving hormone therapy.

- History of treatment with chemotherapy and/or hormonal therapy, or hormone therapy
only.

- No evidence of active/recurrent disease.

- Less than 70 years old at time of recruitment.

- Post-menopausal prior to initial treatment.

- In the judgment of the consenting professional, is able to provide informed consent.

- Patient is able to understand English, through verbal and written communication.

Exclusion Criteria:

- Recurrence of breast cancer or diagnosis of another cancer except basal cell
carcinoma.

- Exposure to chemotherapy or radiation therapy for any medical condition unrelated to
breast cancer.

- Neurobehavioral risk factors including history of neurological disorder, or moderate
to severe head trauma (loss of consciousness > 60 min or evidence of structural brain
changes on imaging).

- Neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease, multiple
sclerosis, etc.

- Self-reported sleep disorders that could influence cognitive functioning including
sleep apnea and chronic fatigue syndrome.

- Self-reported Axis I psychiatric disorder (DSM-IV), major affective disorder
(untreated), bipolar disorder, schizophrenia.

- Disorder (DSM-IV), major affective disorder (untreated), bipolar disorder,
schizophrenia.

- Male.

Healthy Control Inclusion Criteria:

- Have had no diagnosis of cancer except basal cell carcinoma.

- Less than 70 years old at time of recruitment.

- Post-menopausal.

- In the judgment of the consenting professional, is able to provide informed consent.

- Patient is able to understand English, through verbal and written communication

Healthy Control Exclusion Criteria:

- Exposure to chemotherapy or radiation therapy for any medical condition.

- Neurobehavioral risk factors including history of neurological disorder, or moderate
to severe head trauma (loss of consciousness > 60 min or evidence of structural brain
changes on imaging).

- Neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease, multiple
sclerosis etc.

- Self-reported sleep disorders that could influence cognitive functioning including
sleep apnea and chronic fatigue syndrome.

- Self-reported Axis I psychiatric disorder (DSM-IV), major affective disorder
(untreated), bipolar disorder, schizophrenia.

- Male.