Second Echelon Node Study With Methylene Blue

After lymphatic mapping of the SLN with Tc-99 sulfur colloid only, the second-echelon
lymphatic mapping was then performed with methylene blue dye by injecting the SLN with
0.05-0.10 cc non dilute methylene blue and tracing via the efferent lymphatic channel to the
second echelon lymph node (Figure 1). For our initial patients a 27 gauge needle and 3cc
syringe was utilized but this was modified over the study to a 30 gauge needle and 1cc
syringe. The blue efferent lymphatic was traced with minimal spreading of the surrounding
tissue to visualize its drainage into the next lymph node(s). A second echelon lymph node was
considered any blue lymph node or any lymph node with blue dye leading directly into it. The
number of second echelon lymph nodes was recorded. The second-echelon lymph node(s) was not
removed.

Inclusion Criteria:

- Histologically confirmed primary cutaneous melanoma located on the extremity or trunk

- Patients with clinically stage I-II melanoma

- Histologically confirmed invasive ductal or lobular carcinoma

- Patient undergoing surgical treatment of breast primary and sentinel lymph node
biopsy- clinically node negative

Exclusion Criteria:

- Melanoma located on the head or neck, uveal or mucosal

- Previous surgery or radiation in or near the sentinel lymph node biopsy nodal basin

- Preoperative biopsy proven regional lymph node involvement

- Failure of lymphatic mapping with radioactive colloid

- Women who are pregnant or nursing

- Prior ipsilateral axillary surgery or radiation

- Inflammatory breast cancer

- No lymph node identified in the ipsilateral breast during pathologic review of the
mastectomy specimen

- Stage IV breast cancer