Skin Lipid Profiles in Term and Preterm Infants
| Status: | Completed |
|---|---|
| Conditions: | Women's Studies |
| Therapuetic Areas: | Reproductive |
| Healthy: | No |
| Age Range: | Any |
| Updated: | 5/20/2017 |
| Start Date: | May 2010 |
| End Date: | December 2016 |
This study is designed to compare the skin lipid and protein composition between term and
premature infants and determine how the skin composition changes over the first four weeks
of life. The investigators hope to elucidate the unique characteristics of premature skin by
measuring the lipid and protein content in skin, how it changes during the first month of
life, and how it varies with formula feeding versus breast feeding. Additionally, the
investigators will study the relationships among diet, skin composition and plasma lipids in
premature infants over the first four weeks of life.
premature infants and determine how the skin composition changes over the first four weeks
of life. The investigators hope to elucidate the unique characteristics of premature skin by
measuring the lipid and protein content in skin, how it changes during the first month of
life, and how it varies with formula feeding versus breast feeding. Additionally, the
investigators will study the relationships among diet, skin composition and plasma lipids in
premature infants over the first four weeks of life.
Infants in the UCDMC Neonatal Intensive Care Unit with no congenital or acquired diseases of
the skin or cardiovascular system will be enrolled after informed consent has been obtained
from their parents. This is an observational study with no intervention. Procedures: At
enrollment, 2 weeks and 4 weeks of age, skin lipids will be collected using three methods.
First two small pieces of blotting paper (2 cm x 2 cm) will be placed on the infant's skin,
one on the left side of the abdomen just above the umbilicus and one on the left inner
thigh. The blotting paper will be left in place for 15 seconds and then removed. Second, two
adhesive discs will be placed on the skin, one just below the umbilicus and one on the right
inner thigh. The discs will be left in place for 30 seconds and then removed. Third, two
areas of skin, one on the right side of the abdomen just above the umbilicus and the other
on the inner right thigh, will be gently swabbed with sterile cotton swabs (one for each
location). The intent is to remove a thin layer of oil/lipid from the skin upon removal of
the paper or the adhesive disc or with swabbing without disrupting the skin surface (similar
to taking a fingerprint). Blood specimens will be obtained three times, each time 1 ml :
once at enrollment, at 2 weeks and the last one at 4 weeks of age, to generate a plasma
lipoprotein profile and size distribution (HDL, LDL, VLDL, total cholesterol). For infants
that are fed expressed human milk, we will also obtain a sample of mother's milk for
analysis of lipid profile (about 2 ml).
the skin or cardiovascular system will be enrolled after informed consent has been obtained
from their parents. This is an observational study with no intervention. Procedures: At
enrollment, 2 weeks and 4 weeks of age, skin lipids will be collected using three methods.
First two small pieces of blotting paper (2 cm x 2 cm) will be placed on the infant's skin,
one on the left side of the abdomen just above the umbilicus and one on the left inner
thigh. The blotting paper will be left in place for 15 seconds and then removed. Second, two
adhesive discs will be placed on the skin, one just below the umbilicus and one on the right
inner thigh. The discs will be left in place for 30 seconds and then removed. Third, two
areas of skin, one on the right side of the abdomen just above the umbilicus and the other
on the inner right thigh, will be gently swabbed with sterile cotton swabs (one for each
location). The intent is to remove a thin layer of oil/lipid from the skin upon removal of
the paper or the adhesive disc or with swabbing without disrupting the skin surface (similar
to taking a fingerprint). Blood specimens will be obtained three times, each time 1 ml :
once at enrollment, at 2 weeks and the last one at 4 weeks of age, to generate a plasma
lipoprotein profile and size distribution (HDL, LDL, VLDL, total cholesterol). For infants
that are fed expressed human milk, we will also obtain a sample of mother's milk for
analysis of lipid profile (about 2 ml).
Inclusion Criteria:
- infants who are likely to be inpatients in the NICU for at least 4 weeks
Exclusion Criteria:
- congenital or acquired skin disease,
- cyanotic congenital heart disease,
- neonates that are not viable and
- those with lethal anomalies such as anencephaly, trisomy 13, trisomy 18, renal
agenesis
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