Development of Biomarkers of Effect From Chronic Tobacco Usage

This single site, three-group (healthy, adult male smokers (SMK), moist snuff consumers
(MSC), and non-tobacco consumers (NTC), single-blind, cross-sectional study, included one
overnight confinement of study subjects for Test Visit (Visit #3). No lifestyle changes were
required, inclusive of tobacco habits. In addition to the Screening Visit (Visit #1), two
other visits (Acceptance Visit [Visit #2] and a Test Visit [Visit #3]) were conducted at the
clinical study site.

Inclusion Criteria:

1. Males between 35 and 60 years of age, inclusive;

2. Met cohort-specific requirements as follows:

1. Smokers: Must have been exclusive full flavor (FF) (>13 mg FTC [Federal Trade
Commission] "tar") or full flavor low "tar" (FFLT) (6.0 to 10.3 mg FTC "tar")
smokers who self-reported smoking at least 10 cigarettes daily for at least the
past three years prior to screening and whose expired carbon monoxide (ECO) is
10 to 100 parts per million (ppm) at screening and study check-in. Note:
Subjects with an ECO of 2 to 9 ppm may have been included upon joint review by
the Sponsor and the Investigator.

2. Moist Snuff Consumers: Must have been exclusive moist snuff consumers of any
brand (Copenhagen, Skoal, Grizzly, Kodiak, Timber Wolf, Longhorn, Red Man,
etc.), any style (snuff cut, long cut, mid cut, fine cut, or pouch) and any
flavor (natural, straight, mint, wintergreen, etc.) who reported using at least
two can per week for at least the past three years prior to screening and whose
ECO is 0 5 ppm at screening and study check-in. Note: Subjects with an ECO of 6
to 10 ppm may have been included upon joint review by the Sponsor and the
Investigator.

3. Non-Tobacco Consumers: Must have been non-consumers of any tobacco or
nicotine-containing products for at least five years prior to study screening
and whose ECO is 0 to 5 ppm at screening and study check-in. Note: Subjects with
an ECO of 6 to 10 ppm may have been included upon joint review by the Sponsor
and the Investigator.

3. Subjects who are tobacco smokers: who did NOT intend to quit smoking nor intended to
quit using moist snuff. "Intent to quit" was defined as planning a quit attempt
within a month of screening.

4. All subjects must have met the American Red Cross Blood Donation Eligibility
Guidelines (64) (inclusive of, but not limited to, age, blood pressure, bleeding
conditions, recent donation, and weight) and have been generally healthy and felt
well with no restrictions or limitations on activities.

5. Weighed at least 110 pounds.

6. Had a hemoglobin >/= 12.5 g/dL and a hematocrit >/= 38%.

7. Tested negative for drugs of abuse by urine drug screen at screening and at study
check-in, unless subject was able to present proof of a corresponding prescription
concomitant medication.

8. Performed a negative breath alcohol test at screening and study check-in.

9. Had a negative urine cotinine test at screening and study check-in for non-tobacco
consumers.

10. Had a positive urine cotinine test at screening and study check-in for tobacco
consumers.

11. Subjects must, in the opinion of the Investigator, had been free of clinically
significant health problems, including oral health.

12. Not taking daily medication for a chronic medical condition deemed clinically
significant by the Investigator.

13. Not regularly taking creatine supplements.

14. Not have used aspirin (or related drugs, other than aspirin prophylaxis up to 81
mg/day) in the seven days prior to study check-in.

15. Tested negative for Hepatitis B Surface Antigen (HBsAg), negative for Hepatitis C
Antibody (HCAb), and had a negative Human Immunodeficiency Virus (HIV) screen.

16. At least 60 days had passed since the subject's last whole blood (standard) donation.

17. Able to comprehend and willing to sign an Informed Consent Form (ICF).

18. Able to read and comprehend questionnaires in English.

Exclusion Criteria:

Subjects who met any of the following criteria will be excluded from the study:

1. Met cohort specific exclusions as follows:

1. Smokers: For three years prior to the study through Day -1, the use of any
tobacco- or nicotine containing product or device other than FF or FFLT
tobacco-burning cigarettes, including tobacco-heating cigarettes, e cigarettes,
beedis, chewing tobacco, dry snuff, moist snuff, snus, or any nicotine
replacement therapy (NRT) (e.g., patch, gum, lozenge, inhaler, spray, etc.).
NOTE: Subjects who have smoked < 6 cigars in the past 12 months prior to
screening would NOT have been excluded. Subjects who received short-term
administration of a NRT as a temporary bridging treatment would NOT have been
excluded.

2. Moist Snuff Consumers: For three years prior to the study through Day -1, the
use of any other tobacco- or nicotine-containing product or device other than
moist snuff, including tobacco-burning cigarettes, tobacco-heating cigarettes,
e-cigarettes, beedis, cigars, pipes, chewing tobacco, snus, dry snuff, or any
NRT (e.g., patch, gum, lozenge, inhaler, spray, etc.). NOTE: Subjects who
received short-term administration of a NRT as a temporary bridging treatment
would NOT have been excluded.

3. Non Tobacco Consumers: For five years prior to the study through Day -1, the use
of any kind of tobacco products or the use of any non-tobacco
nicotine-containing products (NRTs) or devices (e.g., e-cigarettes).

2. Subjects who were tobacco consumers: expressed an interest in quitting smoking or
using moist snuff (defined as planning a quit attempt within a month of screening).

3. Subjects who were MSC users or non tobacco consumers: an ECO > 5 ppm at screening or
study check-in (ECO of 6 to 10 ppm may not have been excluded based on joint review
by the Sponsor and Investigator).

4. Subjects who were smokers: an ECO < 10 ppm at screening or study check-in (ECO of 2
to 9 ppm may not have been excluded based on joint review by the Sponsor and
Investigator).

5. Any chronic illness that, in the opinion of the Investigator, is unstable.

6. A temperature above 99.5° F.

7. Poor peripheral venous access.

8. Clinically uncontrolled hypertension (>/= 180 mmHg systolic or >/= 100 mmHg diastolic
blood pressure).

9. Diabetes mellitus (either insulin-dependent or non-insulin dependent).

10. Blood sugar levels > 130 mg/dL after the minimum 8 hour fast requested at screening.

11. Peak flow ≤ 70% of predicted average peak expiratory flow (PEF) for age and height.

12. Clinical manifestations of significant metabolic, hepatic, renal, hematological,
pulmonary, cardiovascular, gastrointestinal, urological, neurological, or psychiatric
disorders.

13. History or presence of an abnormal electrocardiogram (ECG), which, in the
Investigator's opinion, is clinically significant.

14. History of alcohol abuse or illicit drug use within one year prior to study entry.
NOTE: Subjects who self-reported drinking less than or equal to 14 servings of
alcoholic beverages per week (1 serving = 12 ounces of beer, 6 ounces of wine, or 1
ounce of liquor) were NOT excluded.

15. Presence of an acute infection, with or without antibiotic treatment.

16. Any history of abnormal bleeding or clotting disorder, or an individual taking any
anticoagulants.

17. Any history of clotting disorder from Factor V who is taking anticoagulants.

18. Cancer that has been treated successfully, but less than 5 years since completion of
treatment, other than skin cancer (exclusionary of melanoma).

19. Squamous or basal cell cancers of the skin that had NOT been completely removed.

20. History of leukemia or lymphoma, including Hodgkin's disease and other cancers of the
blood.

21. Known tuberculosis.

22. Sickle cell disease.

23. Acquired Immune Deficiency Syndrome (AIDS) or a positive HIV antibody test.

24. History of illegal intravenous (IV) drug use.

25. Required use of concomitant medications that were excluded from the study.

26. Less than 12 months since a blood transfusion from another person in the United
States (unless his own "autologous" blood), or exposure to someone else's blood.

27. Less than 6 months since diagnosis and treatment of a significant heart murmur.

28. Less than 12 months since treatment for syphilis or gonorrhea was completed.

29. Less than 60 days since participation in an investigational study in which receipt of
an investigation drug or product occurred.

30. Evidence of visible oral cancer, as found in an oral health examination at the
screening visit.