Bioavailability of Zinc and Iron From a Whey-based Protein Supplement Consumed With a Habitual Plant-based Diet

Study 1: Zinc absorption studies The zinc study employed a 2-day cross-over design, labeling
the WPS diet with a different zinc stable isotope (67Zn) than the control diet (70Zn). All
meals during the 2-day period were labeled with tracer. The dual isotope ratio technique was
used with a 3rd Zn stable isotope (68Zn) given intravenously and urine enrichment of all
isotopes measured on Study Days 6-9 to measure fractional absorption of Zn (FAZ). The amount
of Zn absorbed for the day was determined by multiplying the Zn intake for the day
(determined from lab analyses of duplicate test meals) by the FAZ. Children (n=16) were
randomized as to the order in which they consume the test and control meals on Study Day 1
and 2.

Study 2: Iron absorption studies The iron study was a cross sectional study with one group
receiving control meals and the second group receiving the same control meal plus WPS. Iron
absorption was measured using the erythrocyte iron incorporation technique with labeling of
all meals over 2 days (58Fe). This was preceded by a reference dose of Fe57 and ascorbate on
the previous day. Children (n=32) were randomized to receive the control diet or intervention
diet (control diet + WPS).

Inclusion Criteria:

- 2-3 years of age

- Live in poor, rural communities

- Healthy

- Parents had provided informed consent

Exclusion Criteria:

- An acute or chronic illness which affects gut function, or

- They are breast feeding.