Absorption of Zinc (Zn) From Zn-biofortified and Zn-fortified Maize in Young Zambian Children Between 24-36 Months
| Status: | Completed |
|---|---|
| Conditions: | Food Studies |
| Therapuetic Areas: | Pharmacology / Toxicology |
| Healthy: | No |
| Age Range: | Any |
| Updated: | 4/21/2016 |
| Start Date: | October 2012 |
| End Date: | December 2015 |
The objective of this study is to compare the quantity of Zn absorbed from an accurately
weighed quantity (~100 g) of minimally milled control maize (~15 µg Zn/g maize), from
biofortified maize (~30 µg Zn/g) and from the same control maize that has been fortified
(total level of ~60 µg Zn/g) when fed to young children age 24-36 months whose major
habitual food staple is maize.
weighed quantity (~100 g) of minimally milled control maize (~15 µg Zn/g maize), from
biofortified maize (~30 µg Zn/g) and from the same control maize that has been fortified
(total level of ~60 µg Zn/g) when fed to young children age 24-36 months whose major
habitual food staple is maize.
Participating children were randomized to receive an accurately weighed quantity of
~100g/day of either the control, fortified or biofortified study maize for 1 day.
On day 1, mothers brought their child to the health center before breakfast. All meals were
extrinsically labeled with a zinc stable isotope (70Zn). Meals on this day were consumed in
the presence of the research staff. The quantities of nshima or porridge prepared and eaten
were accurately weighed and duplicate meals were collected for subsequent mineral analyses.
Between lunch and dinner on day 1 at the Health Center, a blood sample was collected for Hb
and plasma Zn analyses and, immediately after and through the same needle, a stable isotope
of zinc was intravenously administered into a forearm vein over 1-2 minutes. The family then
returned home.
Morning spot urine samples were collected twice daily starting on day 5 and continuing
through day 8. Once these collections were finished, the study for this child was complete.
Dietary Zn was measured from laboratory assays of the duplicate test meals. Fractional
absorption of Zn (FAZ) was measured by dual isotope tracer ratio methods in urine. Absorbed
Zn was calculated by multiplying dietary Zn by FAZ.
~100g/day of either the control, fortified or biofortified study maize for 1 day.
On day 1, mothers brought their child to the health center before breakfast. All meals were
extrinsically labeled with a zinc stable isotope (70Zn). Meals on this day were consumed in
the presence of the research staff. The quantities of nshima or porridge prepared and eaten
were accurately weighed and duplicate meals were collected for subsequent mineral analyses.
Between lunch and dinner on day 1 at the Health Center, a blood sample was collected for Hb
and plasma Zn analyses and, immediately after and through the same needle, a stable isotope
of zinc was intravenously administered into a forearm vein over 1-2 minutes. The family then
returned home.
Morning spot urine samples were collected twice daily starting on day 5 and continuing
through day 8. Once these collections were finished, the study for this child was complete.
Dietary Zn was measured from laboratory assays of the duplicate test meals. Fractional
absorption of Zn (FAZ) was measured by dual isotope tracer ratio methods in urine. Absorbed
Zn was calculated by multiplying dietary Zn by FAZ.
Inclusion Criteria:
- Children age 24 to 36 months
- Maize meal accounts for at least 75% energy intake
- Able to consume 100 g maize flour/day
- Clinically healthy (stunting is acceptable)
- Lives in target community
Exclusion Criteria:
- Children with chronic or other known chronic disease
- If children are receiving zinc-fortified products, these products will need to be
withheld for 2 weeks prior to the study
We found this trial at
2
sites
13001 E 17th Pl
Aurora, Colorado 80045
Aurora, Colorado 80045
(303) 724-5000
University of Colorado Anschutz Medical Campus Located in the Denver metro area near the Rocky...
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