Quadrivalent Influenza VLP Vaccine Dose Ranging Study in Young Adults

Inclusion Criteria:

1. Healthy adult male or female, 18-49 years of age

2. Willing and able to give informed consent prior to study enrollment

3. Able to comply with study requirements

4. Women of child-bearing potential must have a negative urine pregnancy test at
vaccination, will be advised through the Informed Consent process to avoid becoming
pregnant over the duration of the study, and must assert that they will employ an
effective form of birth control for the duration of the study. Acceptable forms of
birth control are: credible history of continuous abstinence from heterosexual
activity, or prior surgical sterilization, hormonal contraceptives (oral, injectable,
implant, patch, ring), barrier contraceptives (condom or diaphragm), and intrauterine
device (IUD). Women with an adequately documented history of surgical sterility are
exempt from urine pregnancy testing.

Exclusion Criteria:

1. Any ongoing, symptomatic acute or chronic illness requiring medical or surgical care.

- Asymptomatic conditions or findings (e.g., mild hypertension, dyslipidemia) that
are not associated with evidence of end-organ damage are not exclusionary
provided that they are being appropriately managed and are clinically stable
(i.e., unlikely to result in symptomatic illness within the time-course of this
study) in the opinion of the investigator.

- Acute or chronic illnesses or conditions which may be reasonably predicted to
become symptomatic if treatment were withdrawn or interrupted are exclusionary,
even if stable.

- Acute or chronic illnesses reasonably expected to be associated with increased
risks associated with influenza (e.g., cardio-pulmonary diseases, diabetes
mellitus, renal or hepatic dysfunction, hemoglobinopathies) are exclusionary,
even if stable.

- Note that illnesses or conditions may be exclusionary, even if otherwise stable,
due to therapies used to treat them (see exclusion criteria 2,5,8,9).

2. Participation in research involving investigational product (drug/ biologic/ device)
within 45 days before planned date of first vaccination

3. History of a serious reaction to prior influenza vaccination, known allergy to
constituents of licensed TIV (e.g., egg proteins) or polysorbate-80.

4. History of Guillain-Barré Syndrome (GBS) within 6 weeks following a previous
influenza vaccine.

5. Received any vaccine in the 4 weeks preceding the study vaccination and any influenza
vaccine within six months preceding the study vaccination.

6. History of receipt of any avian influenza vaccine containing an H5 antigen, or known
exposure to birds infected with an H5 virus.

7. Any known or suspected immunosuppressive illness, congenital or acquired, based on
medical history and/or physical examination.

8. Chronic administration (defined as more than 14 continuous days) of
immunosuppressants or other immune-modifying drugs within 6 months prior to the
administration of the study vaccine. An immunosuppressant dose of glucocorticoid will
be defined as a systemic dose greater or equal to 10mg of prednisone per day or
equivalent. The use of topical, inhaled, and nasal glucocorticoids will be permitted.

9. Administration of immunoglobulins and/or any blood products within the three months
preceding the administration of the study vaccine or during the study.

10. Acute disease at the time of enrollment (defined as the presence of a moderate or
severe illness with or without fever, or an oral temperature >38.0°C on the planned
day of vaccine administration.

11. Any condition that in the opinion of the investigator would pose a health risk to the
subject if enrolled or could interfere with evaluation of the vaccine or
interpretation of study results (including neurologic or psychiatric conditions
deemed likely to impair the quality of safety reporting).

12. Known disturbance of coagulation.

13. Women who are breastfeeding or plan to become pregnant during the study.

14. Suspicion or recent history (within one year of planned vaccination) of alcohol or
other substance abuse.