The Role of Dietary Fat on Postprandial Endotoxemia in Healthy Adults
| Status: | Completed |
|---|---|
| Conditions: | Infectious Disease |
| Therapuetic Areas: | Immunology / Infectious Diseases |
| Healthy: | No |
| Age Range: | 18 - 40 |
| Updated: | 4/21/2016 |
| Start Date: | April 2014 |
| End Date: | April 2015 |
Dietary Fat Affects Postprandial Serum Endotoxin Concentration in Healthy Adults
The purpose of this study was to determine the effect of different dietary fats (saturated
or unsaturated) on postprandial endotoxemia and systemic low grade acute inflammation. The
investigators hypothesized that meals rich in saturated or n-6 fatty acids would increase
postprandial endotoxemia but meals high in n-3 fatty acids would decrease postprandial
endotoxemia.Participants were recruited via email and randomized to treatment meal in this
single-blind, cross-over study. Each test session participants reported to the laboratory
right away in the morning. An indwelling catheter was inserted into the participant
non-dominant arm by a qualified nurse and a baseline blood draw was taken. The participant
was then provided with one of four test meals (a porridge-type meal containing a different
dietary fat), which they ate in entirety within 15 minutes. The participants remained in the
laboratory for the next five and a half hours and were not allowed to consume any food or
drink except water. During this time, further blood draws were taken at intervals of one
hour for a total of five hours after the consumption of the test meal. Collected blood was
processed on-site and the serum fraction collected and tested for endotoxin, inflammatory
biomarkers, and metabolites.
or unsaturated) on postprandial endotoxemia and systemic low grade acute inflammation. The
investigators hypothesized that meals rich in saturated or n-6 fatty acids would increase
postprandial endotoxemia but meals high in n-3 fatty acids would decrease postprandial
endotoxemia.Participants were recruited via email and randomized to treatment meal in this
single-blind, cross-over study. Each test session participants reported to the laboratory
right away in the morning. An indwelling catheter was inserted into the participant
non-dominant arm by a qualified nurse and a baseline blood draw was taken. The participant
was then provided with one of four test meals (a porridge-type meal containing a different
dietary fat), which they ate in entirety within 15 minutes. The participants remained in the
laboratory for the next five and a half hours and were not allowed to consume any food or
drink except water. During this time, further blood draws were taken at intervals of one
hour for a total of five hours after the consumption of the test meal. Collected blood was
processed on-site and the serum fraction collected and tested for endotoxin, inflammatory
biomarkers, and metabolites.
Inclusion Criteria:
- Aged between 18 and 40 years old;
- Willingness to eat test meals;
- Body mass index ≥ 19.9 ±0.8 and ≤ 24.9 ±0.8;
- Weight stable (< 2 kilogram weight change in the previous 3 months)
Exclusion Criteria:
- Presence of acute or chronic disease;
- Use of tobacco products;
- Consumes more than 21 units of alcohol per week;
- Use of anti-inflammatory medication;
- History suggestive of macronutrient malabsorption
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