EEG/ERP Longitudinal Study in Alzheimer's Disease (AD)

In study NCT00582127, two age-matched cohorts (mild AD, n=98 and healthy controls, n=100)
were recruited to 7 clinical sites to undergo a broad range of clinical, neuropsychological,
and neuroelectrophysiological tests. The complete battery of ADNI neuropsychological tests
were performed on the mild AD subjects as well as standard blood tests and MRI using the
ADNI protocol. MRI volumetry analysis was performed on the MRI data. Both cohorts underwent
a 3-minute EEG recording and event-related potential (ERP) testing using a standard
2-deviant auditory oddball paradigm with the COGNISION(tm) System from Neuronetrix. The
study showed that specific EEG/ERP features could be used to discriminate the mild AD cohort
from healthy controls.

The current study is intended as a follow up to NCT00582127.AD subjects that successfully
completed COGNISION™ and psychometric testing in the previous study are eligible to be part
of the present follow up.

In the first part of the current study, de-identified cognitive and functional data for the
AD patients that the sites have collected during regular check ups after testing for the
original Neuronetrix study will be shared with the study Sponsor. The retrospective chart
review has three main objectives:

- Quantify the rate of cognitive and functional decline in these patients.

- Investigate whether ERP and/or qEEG data collected during testing for the original
Neuronetrix study can provide any insight on subsequent rate of cognitive and
functional decline.

- Identify study subjects that might still be functional enough to successfully complete
a follow-up ERP/qEEG test. These subjects will be offered an opportunity to come back
to the sites for follow-up ERP/qEEG and psychometric testing.

Up to 40 subjects will be invited back to the sites. Subjects that choose to enroll in the
second part of the study will have an ERP/qEEG test administered by a Neuronetrix certified
test administrator. Subjects that complete the test successfully will move on to clinical
evaluation and psychometric testing.

Results obtained from this part of the study will be compared with ERP/qEEG and psychometric
data from the completed Neuronetrix study mentioned above. The two main objectives of the
comparisons will be:

- Investigate whether ERP and qEEG measures collected in outpatient settings show
significant changes over time in subjects with AD.

- Correlate longitudinal changes in ERP and qEEG measures (if any) with cognitive and
functional decline as measured by psychometric tests.

Inclusion criteria:

Between 60 and 95 years old (inclusive) A diagnosis of Alzheimer's disease A successful
ERP/EEG test performed with the COGNISION™ system 2-5 years prior to testing for the
current study

A sub-group of up to 40 subjects will be called back to the sites for follow-up ERP/qEEG
and psychometric testing. Additional inclusion criteria for these subjects will be:

- Willing and able to undergo ERP/EEG and psychometric testing

- Have a study partner able to provide an independent evaluation of functioning

- Permitted medications stable for at least 2 weeks prior to testing

Exclusion Criteria:

Subjects will not be called back to sites for follow-up testing if they have:

- Known allergy to latex

- Neuropsychological tests administered within three months of study enrollment

- Investigational agents administered within three months of study enrollment

- Severe cognitive and functional deficits that in the judgement of the site PI will
prevent them from successfully completing ERP/EEG and/or psychometric testing

- Any significant systemic illness or unstable medical condition at the time of testing
which could lead to difficulty complying with the protocol

- Use of psychoactive substances (Gingko Biloba, melatonin, sedatives etc.) will be
allowed as long as the subject suspends their use on the day of testing. Smokers will
not be required to abstain.