Biomarkers in Tissue Samples From Young Patients With Liver Cancer

PRIMARY OBJECTIVES:

I. To identify biomarker signatures associated with hepatoblastoma subtypes (by microRNA
array profiling), and their potential correlation with liver developmental stages as well as
outcome.

II. To correlate expression array data with copy number array data.

SECONDARY OBJECTIVES:

I. To carry out a focused sequencing interrogation of amplified or deleted genomic regions
as well as genes in key cell pathways involved in embryonic development and/or stem cell
signaling pathways for which preliminary data suggests involvement in the pathogenesis of
hepatoblastoma.

II. To perform whole exome sequencing in a subset of tumors.

OUTLINE: This is a multicenter study.

Archived tumor tissue samples are analyzed for hepatoblastoma and other liver tumor
biomarkers by microRNA array profiling and exome sequencing. Results are then compared with
patients' clinical data.

Inclusion Criteria:

- Diagnosis of hepatoblastoma or other liver cancer including the following subtypes:

- Pure fetal histology

- Transitional histology

- Small cell undifferentiated histology

- Participated on the Children's Oncology Group (COG) clinical trials

- Available data and tumor samples