Cryotherapy and GM-CSF in Treating Patients With Lung Metastases or Primary Lung Cancer

OBJECTIVES:

Primary

- Determine whether percutaneous cryotherapy in combination with aerosolized sargramostim
(GM-CSF) has any demonstrable immunologic effect in patients with pulmonary metastases
or primary lung cancer.

- Determine whether any systemic immune response is detectable by the combination of
cryotherapy as the antigen presentation source and GM-CSF as the immunologic adjuvant.

- Determine whether low morbidities will be maintained in patients treated with this
regimen.

- Determine whether effective immunization is associated with a drop in CD4+, CD25+,
LTP(TGF-β1)+, Tr cells as measured by flow cytometry or ELISPOT assay for
TGF-β1-secreting cells.

Secondary

- Determine clinical response (i.e., tumor control in the dominant masses undergoing
cryotherapy or in other metastatic sites) as measured by CT criteria.

- Determine the toxicity of this regimen in these patients.

OUTLINE: Patients undergo CT-guided core biopsy of a dominant lung mass and placement of at
least 2 cryoprobes. Prior to initiating the freeze, patients receive an interstitial
injection of sargramostim (GM-CSF) near the tumor. Patients then undergo percutaneous
cryotherapy over 2 hours utilizing a freeze-thaw-freeze cycle. Beginning within 3 days of
cryotherapy, patients receive aerosolized GM-CSF twice daily for 1 week. Beginning on day 32,
patients may elect to undergo a second course of treatment as described above in the absence
of disease progression or unacceptable toxicity.

Patients undergo blood and tumor tissue collection at baseline and periodically during study
for immunological correlative studies. Peripheral blood mononuclear cells isolated from blood
samples are analyzed for antigen-specific CD4-positive or CD8-positive T-cell response by
flow cytometry or by TGF-β1 ELISPOT assay to measure TGF-β1- secreting cells. Tumor cell
lysates extracted from tumor samples are pulsed with autologous dendritic cells and analyzed
by ELISPOT assay to measure T-cell reactivity in tumor specimens.

After completion of study therapy, patients are followed at 6 and 12 months.

DISEASE CHARACTERISTICS:

- Histologically confirmed diagnosis of 1 of the following:

- Primary non-small cell lung cancer (NSCLC)

- Any stage nonoperative NSCLC or patient refuses surgery

- Any cancer with pulmonary metastatic disease (including renal cell cancer)

- Stage IV disease (any T, any N, M1)

- Must have 1-10 pulmonary or mediastinal masses meeting the following criteria:

- At least 1 mass is appropriate for 2 sessions of core biopsy and cryotherapy with
relatively easy access/low risk in nonoperative patients (or those refusing
surgery)

- The two dominant masses are defined as either the largest and/or those that may
cause imminent morbidity from continued local progression, thereby potentially
benefiting from thoracic cryotherapy alone

- Optimal tumor size > 1.0 cm

- Dominant masses up to 6 cm in diameter may be considered if thorough
cryotherapy coverage can be anticipated with minimal additional treatment
morbidity

- Measurable disease, defined as tridimensional measurements of up to 6 different
pulmonary or mediastinal masses ≥ 0.5 cm by CT scan

- No active pleural effusion that could be related to respiratory infection or requires
further work-up

- No untreated and/or unstable brain metastases

PATIENT CHARACTERISTICS:

- Karnofsky performance status 70-100%

- Life expectancy ≥ 12 weeks

- Granulocyte count ≥ 1,500/mm³

- Platelet count ≥ 50,000/mm³

- INR < 1.5 (i.e., normal PT/PTT)

- Hemoglobin ≥ 8.0 g/dL

- Bilirubin ≤ 2 times upper limit of normal (ULN)

- AST ≤ 3 times ULN

- Satisfactory pulmonary function test as determined by supervising oncologist, thoracic
surgeon, or pulmonologist

- Not pregnant or lactating

- Negative pregnancy test

- Fertile patients must use effective contraception

- No other active malignancy except nonmelanoma skin cancer or carcinoma in situ of the
cervix

- Inactive history of cancer allowed if the patient has been disease-free for > 2
years

- No serious medical or psychiatric illnesses that would preclude informed consent or
limit survival to < 12 wks

- No uncontrollable cough or inability to lie flat

- No New York Heart Association class III or IV heart disease

- No known immunodeficiency state

- No uncontrolled infection

- No uncontrolled coagulopathy or bleeding diathesis

- No advance directive that would prevent the investigator from treating the participant
in the event of a complication occurring during or after the procedure

- No medical contraindication or potential problem that would preclude protocol
compliance

PRIOR CONCURRENT THERAPY:

- More than 4 weeks since prior biologic therapy

- More than 4 weeks since prior immunotherapy

- More than 4 weeks since prior filgrastim (G-CSF) or sargramostim (GM-CSF)

- More than 4 weeks since prior chemotherapy (6 weeks for nitrosoureas or mitomycin)

- More than 4 weeks since prior radiotherapy

- More than 2 weeks since prior corticosteroids

- More than 1 week since prior parenteral antibiotics

- At least 1 week since prior aspirin or aspirin-like medications

- At least 3 days since prior warfarin, clopidogrel bisulfate, or similar compounds

- No concurrent GM-CSF other than study drug

- No concurrent G-CSF

- No concurrent radiotherapy

- No concurrent glucocorticosteroids

- No concurrent parenteral antibiotics

- No concurrent immunosuppressive agents

- No concurrent drugs that cause bleeding tendencies

- No other concurrent biologic therapy, immunotherapy, radiotherapy, or chemotherapy