An Open-label, Single-centre Study Evaluating the Pharmacokinetics of Digoxin Alone and When Administered at Various Doses of Ezogabine/Retigabine in Healthy Adults. The Pharmacokinetics of Ezogabine/Retigabine and the N-acetyl Metabolite of Ezogabine/Retigabine (NAMR) Will Also be Assessed

This is an open-label, single-centre study to evaluate the pharmacokinetics of single-dose
digoxin alone and when administered at various up titration doses of ezogabine/retigabine in
healthy adult subjects. The pharmacokinetics of ezogabine/retigabine and the n-acetyl
metabolite of ezogabine/retigabine (NAMR) will also be assessed.

Inclusion Criteria:

- Male or female between 18 and 65 years of age inclusive, at the time of signing the
informed consent.

- Healthy as determined by a responsible and experienced physician, based on a medical
evaluation including medical history, physical examination, laboratory tests and
cardiac monitoring. A subject with a clinical abnormality or laboratory parameters
outside the reference range for the population being studied may be included only if
the Investigator and the GSK Medical Monitor agree that the finding is unlikely to
introduce additional risk factors and will not interfere with the study procedures.

- A female subject is eligible to participate if she is of: Non-childbearing potential
defined as pre-menopausal females with a documented tubal ligation or hysterectomy; or
postmenopausal defined as 12 months of spontaneous amenorrhea [in questionable cases a
blood sample with simultaneous follicle stimulating hormone (FSH) greater than 40
MlU/ml and estradiol less than 40 pg/ml (less than 147 pmol/L) is confirmatory].
Females on hormone replacement therapy (HRT) and whose menopausal status is in doubt
will be required to use one of the contraception methods in the protocol if they wish
to continue their HRT during the study. Otherwise, they must discontinue HRT to allow
confirmation of post-menopausal status prior to study enrollment. For most forms of
HRT, at least 2-4 weeks should elapse between the cessation of therapy and the blood
draw; this interval depends on the type and dosage of HRT. Following confirmation of
their post-menopausal status, they can resume use of HRT during the study without use
of a contraceptive method; Child-bearing potential and agrees to use one of the
contraception methods listed in the protocol for an appropriate period of time (as
determined by the product label or investigator) prior to the start of dosing to
sufficiently minimize the risk of pregnancy at that point. Female subjects must agree
to use contraception until 1 week post-last dose of ezogabine/retigabine.

- Male subjects with female partners of child-bearing potential must agree to use one of
the contraception methods listed in the protocol. This criterion must be followed from
the time of the first dose of study medication until at least 1 week post-last dose.

- BMI of 18 to 30 kg/m2 and total body weight greater than 50 kg (110 lbs).

- Normal creatinine clearance assessed by the Cockcroft-Gault Method.

- Subject's aspartate aminotransferase, ALT, alkaline phosphatase, and bilirubin are
greater than and equal to 1.5 × ULN (isolated bilirubin greater than 1.5 × ULN is
acceptable if bilirubin is fractionated and direct bilirubin less than 35%).

- Subject is a nonsmoker (defined as a nonsmoker during the last 3 months prior to
Screening and have a negative cotinine test at screening).

- Subject has a negative drug screen (amphetamines, barbiturates, cocaine metabolites,
opiates, benzodiazepines, and cannabinoids) at Screening and at check-in at Day -1.

- Subject is capable of giving written informed consent, which includes compliance with
the requirements and restrictions listed in the consent form.

Exclusion Criteria:

- Subject has positive test results for hepatitis B surface antigen, positive hepatitis
C virus, or human immunodeficiency virus (HIV)-1 or -2 at Screening

- Has active suicidal plan/intent or has had active suicidal thoughts in the past 6
months. Has history of suicide attempt in the last 2 years or more than 1 lifetime
suicide attempt.

- Presence of proteinuria (at least ++) or hematuria or other clinically significant
findings in urinalysis at screening.

- Has a history of urinary retention or risk factors for urinary retention that in the
Investigator's judgment could potentially affect subject safety.

- Subject has a history of syncope, clinically significant palpitations, bradyarrhythmia
or tachyarrhythmia conduction abnormality (e.g., atrioventricular block of any degree,
e.g., left bundle-branch block, right bundle-branch block), or Wolf-ParkinsonWhite
syndrome, or any clinically relevant abnormality identified on the screening medical
assessment, laboratory examination, ECG, or immunogenicity test

- Subject's blood pressure is greater than and equal to 140/90 mm Hg or heart rate is
greater than 100 beats/min at Screening; repeat blood pressures should be taken if the
subject's blood pressure is greater than and equal to 140/90 mm Hg and if the results
are consistently greater than and equal to 140/90 mm Hg, then the subject should be
excluded and advised to consult a physician.

- Subject's QTc interval is greater than 450 ms (per ECG machine interpretation) at
screening; repeat measurement should be taken if the QTc greater than 450 ms and if
the results are consistently greater than 450 ms, then the subject should be excluded

- Female subject is pregnant or breast-feeding

- Subject has a history of any anaphylactic reaction to any drug or a known
hypersensitivity to: LANOXIN/digoxin and/or its excipients, or other digoxin-like
medications; or Ezogabine/retigabine; History of sensitivity to any of the study
medications, or components thereof or a history of drug or other allergy that, in the
opinion of the investigator or GSK Medical Monitor, contraindicates their
participation.

- Subject has a history of significant cardiovascular or pulmonary dysfunction prior to
Screening

- Subject has a fasting triglyceride level greater than 300 mg/dL at Screening

- Subject has a current or chronic history of liver disease, or known hepatic or biliary
abnormalities (with the exception of Gilbert syndrome or asymptomatic gallstones)

- Subject has a history of alcohol or substance abuse within the last 2 years

- Subject has donated blood in the excess of 500 mL within 56 days prior to dosing or
intention of donating in the month after completing the study

- Subject has a history of gastrointestinal surgery which could influence gastric
emptying (e.g., gastrectomy, gastric bypass)

- Subject has a history of inflammatory bowel disease or irritable bowel syndrome

- Subject has received an investigational drug within 30 days prior to dosing with study
medication or 5 half-lives, whichever is longer

- Subject is unlikely to comply with the study protocol and/or to complete the study or
required study procedures or is unlikely or unable to return for follow-up visits

- Subject has used prescription medications or over-the-counter medications, herbal
supplements, or multivitamins within 14 days prior to dosing with any study medication
(beginning with the first dose of digoxin) (see Section 9.2 for additional details)

- Subject has consumed grapefruit juice, cranberry products (such as juice, fruit, or
nutritional supplements), or alcohol, caffeine , or xanthine-containing products
within 7 days (or 5 elimination half lives whichever is shorter) prior to start the
dosing with study medication