CSI-Glucagon for Prevention of Hypoglycemia in Children With Congenital Hyperinsulinism

This is a Phase 2, multi-center, randomized, placebo-controlled, double-blind parallel group
study with open-label follow-up designed to evaluate the efficacy of CSI-Glucagon™ for the
prevention of hypoglycemia with lower IV glucose infusion rates when delivered subcutaneously
to patients up to 1 year of age with congenital hyperinsulinism. CSI-Glucagon™ is expected to
provide a better inpatient treatment option compared to the current standard of care.

The study will consist of three phases:

1. Baseline Phase: First is a baseline stabilization phase during which concomitant therapy
with octreotide and diazoxide will be safely weaned and continuous enteric feed will be
held constant to the degree possible, with the only factors varying being meal size and
IV glucose infusion rate (GIR) adjusted by a set plasma glucose measurement driven
algorithm.

2. Blinded, Randomized Treatment Phase: Following the stabilization phase, subjects will be
randomly assigned to blinded treatment with either glucagon or placebo, which will be
delivered for up to 48 hours with an OmniPod® infusion pump with the controller set to a
starting basal rate for glucagon of 5 μg/kg/hr and GIR adjustments used to maintain
euglycemia. After 48 hours of blinded treatment, all subjects will transition to
open-label active treatment. However, if GIR reduction from baseline is < 20% at 24
hours, subjects will be transitioned early to the open-label phase.

3. Open-label Treatment Phase: The third study period will involve use of CSI-Glucagon™ to
manage blood glucose with minimal GIR for up to 28 days of cumulative exposure.

Inclusion Criteria:

1. Diagnosed with hyperinsulinism:

a. Biochemical; detectable insulin (i.e., ≥1 µIU/L) at time of hypoglycemia (i.e,
blood glucose <50 mg/dl), and/or suppressed free fatty acids (FFA), and/or suppressed
beta-hydroxybutyrate (BOHB) and/or glycemic response to glucagon at time of
hypoglycemia.

2. Absolute necessity of intravenous glucose to prevent hypoglycemia:

1. Having failed diazoxide therapy as defined by inadequacy of 5 days maximum dose
of diazoxide to eliminate the need for IV glucose, not necessarily that diazoxide
has no effect.

2. May be on diazoxide and/or octreotide, but these drugs will be weaned off prior
to randomization.

3. May be on dextrose feeds.

3. Patient may be a participant in other study protocols such as observational studies,
as long as no investigational intervention has taken place within 24 hrs. prior to
screening.

4. Less than 12 months of age at screening.

Exclusion Criteria:

1. History of allergy to glucagon or excipients in the CSI-Glucagon formulation.

2. Currently receiving, or less than 12 hours removed from IV glucagon treatment that
resulted in a best achievable GIR > 8 mg/(kg*min), prior to the start of study drug.

3. Diazoxide naïve or within five days of starting diazoxide.

4. Receiving steroids at doses larger than 20 mg/m2/day (hydrocortisone equivalent).

5. Patients with sepsis.

6. Receiving alpha or beta agonists for blood pressure support.

7. Received an investigational or other study drug within 5 half-lives of drug.

8. Body weight less than or equal to 2.3 kg/5.0 lbs.

9. History of pancreatectomy and GIR < 8 mg/(kg*min) after weaning of all concomitant
therapies.