A Phase II Study of High Dose Bolus IL2 in Patients With Inoperable Stage III or Stage IV Melanoma Who Have Failed Prior Anti-PD1 Immunotherapy: Efficacy and Biomarker Study

This is a phase II study of high dose bolus interleukin-2 (HD IL2) in patients with advanced
inoperable stage III or stage IV melanoma who have prior anti-PD1 immunotherapy.

Each course consists of 2 cycles of HD IL2 as follows: high-dose IL2 at 600,000 IU/kg is
given intravenously (IV) every 8 hours for up to 14 doses (one cycle), followed by a rest
period of 1-2 weeks and readmission for a second HD IL2 cycle for up to 14 doses (second
cycle).

The planned treatment consists of 3 courses (6 cycles) of HD IL-2. Response assessment will
occur at the end of each course of therapy and patients without evidence of disease
progression (Response Evaluation Criteria in Solid Tumors, RECIST, version 1.1) or limiting
toxicities will be offered additional courses of treatment of HD IL2 for a maximum of 3
courses.

Inclusion Criteria:

- Patients must have histologically or cytologically confirmed metastatic melanoma. This
includes American Joint Committee on Cancer (AJCC) stage IV or advanced/inoperable
stage III. This also includes patients with a history of lower stage melanoma and
subsequent recurrent metastatic disease that is either locally/regionally
advanced/inoperable disease or distant metastases.

- Patients must have measurable disease, according to RECIST version 1.1

- Patients must be free of active brain metastasis by contrast-enhanced CT/MRI scans
within 4 weeks prior to enrollment. If known to have prior brain metastases, these
must have been adequately managed with standard of care radiation therapy,
stereotactic radiosurgery or surgery prior to registration on the study.

- A patient must have previously received anti-PD1 immunotherapy (nivolumab or
pembrolizumab) and later experienced disease progression, within 3 months of
registration on this study.

- Patients must not have received systemic therapy or radiotherapy within the preceding
3 weeks. Patients must have recovered from adverse events from previous therapy by the
time registration.

- Patients must be at least 4 weeks from major surgery and have fully recovered from any
effects of surgery, and be free of significant detectable infection prior to
registration.

- For patients who have received prior anti-CTLA4 monoclonal antibody therapy
(ipilimumab or tremelimumab), there is a risk of bowel perforation with IL-2 therapy.
Therefore, for these patients if they have a history of colitis or diarrhea during
anti-CTLA4 monoclonal antibody therapy, it is recommended that they have a formal
evaluation by a gastroenterologist and a colonoscopy/endoscopy should be considered to
demonstrate the absence of active bowel inflammation before initiating IL-2 therapy on
this protocol.

- Life expectancy of greater than 3 months in the opinion of the investigator.

- Eastern Cooperative Oncology Group (ECOG) performance status 0 or 1 (Karnofsky >/=70%)

- Patients must have normal organ and marrow function as defined in the clinical trial
protocol.

- Patients on full-dose anticoagulants (e.g., warfarin) with prothrombin time (PT)/
international normalized ratio (INR) >1.5 are eligible provided that both of the
following criteria are met: (a) The patient has an in-range INR (usually between 2 and
3) on a stable dose of oral anticoagulant or on a stable dose of low molecular weight
heparin. (b) The patient has no active bleeding or pathological condition that carries
a high risk of bleeding (e.g., tumor involving major vessels or known varices).

- Pulmonary: forced expiratory volume at one second (FEV1) > 2.0 liters or > 75% of
predicted for height and age. Pulmonary function tests (PFTs) are required for
patients over 50 years old or with significant pulmonary or smoking history.

- Cardiac: No evidence of congestive heart failure, symptoms of coronary artery disease,
myocardial infarction less than 6 months prior to entry, serious cardiac arrhythmias,
or unstable angina. Patients who are over 40 years old or have had previous myocardial
infarction greater than 6 months prior to study entry or have significant cardiac
family history (CAD or serious arrhythmias) will be required to have a negative or low
probability cardiac stress test (for example, thallium stress test, stress multigated
acquisition (MUGA), stress echo or exercise stress test) for cardiac ischemia within 8
weeks prior to registration.

- Central Nervous System (CNS): No history of cerebrovascular accident or transient
ischemic attacks within the past 6 months from registration.

- Women of child-bearing potential and men must agree to use adequate contraception
(hormonal or barrier method of birth control; abstinence) prior to study entry, for
the duration of study participation, and for at least 6 months after completion of
study therapy. Should a woman become pregnant or suspect she is pregnant while
participating in this study, she should inform her treating physician immediately.

- Women should not be lactating and, if of childbearing age, should have a negative
pregnancy test (b-HCG test; serum or urine, minimum sensitivity 25 IU/L or equivalent
units of b-HCG) within two week of registration in the study.

- Ability to understand and the willingness to sign a written informed consent document.

Exclusion Criteria:

- Patients who have had systemic therapy for melanoma or radiotherapy within 3 weeks
prior to registering on the study or those who have not recovered from adverse events
due to agents administered more than 3 weeks earlier. Patients with a history of
endocrinopathies (e.g. hypothyroidism, adrenal insufficiency, hypopituitarism) are
eligible if they are stable on hormone replacement therapy.

- Patients may not be receiving any other investigational agents.

- Patients with active brain metastases should be excluded from this clinical trial
except as noted above.

- Patients with clinically significant cardiovascular or cerebrovascular disease:

1. history of cerebrovascular accident or transient ischemic attack within past 6
months from registration.

2. myocardial infarction, coronary artery bypass grafting (CABG) or unstable angina
within the past 6 Months from registration.

3. New York Heart Association grade III or greater congestive heart failure, serious
cardiac arrhythmia requiring medication, unstable angina pectoris within past 6
months from registration.

4. clinically significant peripheral vascular disease within past 6 months from
registration.

- PT INR >1.5 unless the patient is on full-dose warfarin.

- Uncontrolled intercurrent illness including, but not limited to, ongoing or active
infection or psychiatric illness/social situations that would limit compliance with
study requirements.

- Patients who have other current malignancies are not eligible. Patients with other
malignancies are eligible if they have been continuously disease free for > 2 years
prior to the time of registration. Patients with prior history at any time of any in
situ cancer, lobular carcinoma of the breast in situ, cervical cancer in situ,
atypical melanocytic hyperplasia or melanoma in situ are eligible. Patients with prior
history of basal or squamous skin cancer are eligible. Patients who have had multiple
primary melanomas are eligible.

- Patients must not have autoimmune disorders or conditions of immunosuppression that
require current ongoing treatment with systemic corticosteroids (or other systemic
immunosuppressants), including oral steroids (i.e., prednisone, dexamethasone) or
continuous use of topical steroid creams or ointments or ophthalmologic steroids or
steroid inhalers. If a patient had been taking steroids, at least 2 weeks must have
passed since the last dose. Patients stable on physiologic replacement doses of
steroids or other forms of hormone replacement therapy are eligible.