A Pilot Study of Immunization With HIV-1 Antigen Pulsed Allogenic Dendritic Cells in HIV-Infected Asymptomatic Patients With CD4+ T Cells > 350 Cells/mm3

Dendritic cells are a type of white blood cell used by the body to fight infection. They are
instrumental in presenting antigens (such as HIV antigens) to the body's immune system.
Since dendritic cells are not functioning maximally in HIV-infected patients, infusion of
dendritic cells from an HIV-negative sibling may enable the affected sibling's immune system
to recognize foreign particles more readily and increase immune response against the virus.

Dendritic cells from an HIV-negative sibling are obtained and treated with various viral
proteins (HIV vaccines) or immunomodulators. The treated dendritic cells are infused into
the HIV-infected patient monthly for 6 months. Siblings must be able to donate on multiple
occasions, and patients are followed every 2-4 weeks during the study. Patients are screened
over 3 months prior to study entry.

Inclusion Criteria

Patients must have:

- HLA A2+.

- Same cell type as donor sibling.

- CD4 count > 350 cells/mm3.

- HIV asymptomatic status.

- No HIV antivirals during study.

- Normal labs and chest x-ray.

Donor siblings must have:

- HLA A2+.

- HIV negativity.

- Ability to donate cells on multiple occasions.

- Negative status for hepatitis B and C.

Exclusion Criteria

Concurrent Medication:

Excluded:

- Antiviral therapy (unless CD4 count declines to < 350 cells/mm3).

Prior Medication:

Excluded:

- Antiviral therapy within 90 days prior to study entry.