Daily Consumption of Well-Cooked Broccoli May Affect Glucosinolate Metabolites and Inflammatory Biomarkers
| Status: | Recruiting |
|---|---|
| Conditions: | Healthy Studies |
| Therapuetic Areas: | Other |
| Healthy: | No |
| Age Range: | 21 - 70 |
| Updated: | 1/11/2017 |
| Start Date: | January 2017 |
| End Date: | May 2017 |
| Contact: | Craig Charron, PhD |
| Email: | craig.charron@ars.usda.gov |
The objectives of the study are 1) to determine the influence of daily consumption of
well-cooked broccoli on plasma and urinary glucosinolate metabolites, and 2) to determine
inflammatory marker changes consistent with decreased cancer risk.
well-cooked broccoli on plasma and urinary glucosinolate metabolites, and 2) to determine
inflammatory marker changes consistent with decreased cancer risk.
Consumption of Brassica vegetables is inversely associated with incidence of several
cancers, including cancer of the lung, stomach, liver, colon, rectum, breast, endometrium,
and ovaries. Brassica vegetables are a good source of many nutrients, but the unique
characteristic of Brassicas (Broccoli in particular) is their rich content of
glucosinolates. Glucosinolates are sulfur-containing compounds that are converted to
isothiocyanates (ITC) by an enzyme in the plant called myrosinase, which is released when
the vesicles containing myrosinase are ruptured by chewing or cutting. The isothiocyanates
are considered to be the active agent for cancer prevention. Some of the mechanisms by which
isothiocyanates likely inhibit cancer include modulation of cytochrome P450 enzymes,
induction of phase II enzymes, and apoptosis.
The aim of this study is to investigate how daily consumption of broccoli with myrosinase
inactivated by cooking influences glucosinolate metabolism and absorption, and consequent
regulation of inflammatory markers.
cancers, including cancer of the lung, stomach, liver, colon, rectum, breast, endometrium,
and ovaries. Brassica vegetables are a good source of many nutrients, but the unique
characteristic of Brassicas (Broccoli in particular) is their rich content of
glucosinolates. Glucosinolates are sulfur-containing compounds that are converted to
isothiocyanates (ITC) by an enzyme in the plant called myrosinase, which is released when
the vesicles containing myrosinase are ruptured by chewing or cutting. The isothiocyanates
are considered to be the active agent for cancer prevention. Some of the mechanisms by which
isothiocyanates likely inhibit cancer include modulation of cytochrome P450 enzymes,
induction of phase II enzymes, and apoptosis.
The aim of this study is to investigate how daily consumption of broccoli with myrosinase
inactivated by cooking influences glucosinolate metabolism and absorption, and consequent
regulation of inflammatory markers.
Inclusion Criteria:
- Non tobacco user
- Cancer Free
- Not currently taking glucosinolate/isothiocyanate containing supplements
Exclusion Criteria:
- Type 2 diabetes requiring the use of diabetes pills, insulin, or non-insulin shots
- Use of blood-thinning medications such as Coumadin (warfarin), Dicumarol, or Miradon
(anisinidione)
- History of bariatric surgery or nutrient malabsorption disease
- Pregnant, lactating, or intending to become pregnant during the study period
- Crohn's disease or diverticulitis
- Suspected or known strictures, fistulas or physiological/mechanical GI obstruction
- Self-report of alcohol or substance abuse within the past 12 months and/or current
acute treatment or rehabilitation program for these problems (long-term participation
in Alcoholics Anonymous is not an exclusion)
We found this trial at
1
site
Beltsville, Maryland 20705
Principal Investigator: Craig Charron, Ph.D.
Click here to add this to my saved trials
