Drug-Drug Interaction Study of AG120 in Healthy Subjects

Inclusion Criteria:

1. Healthy, adult, male or female, 18 - 55 years of age, inclusive, at screening.

2. Continuous non smoker who has not used nicotine containing products for at least 3
months prior to the first dose and throughout the study.

3. BMI ≥ 18.5 and ≤ 32.0 kg/m2 at screening.

4. Medically healthy with no clinically significant medical history, physical
examination, laboratory profiles, vital signs or ECGs, as deemed by the PI or
designee.

5. For a female of childbearing potential: either be sexually inactive (abstinent) for
14 days prior to the first dose and throughout the study or be using one of the
following acceptable birth control methods:

- non-hormone releasing intrauterine device for at least 3 months prior to the
first dose and with either a physical (e.g. condom, diaphragm, or other) or a
chemical (e.g., spermicide) barrier method from the time of screening and
throughout the study.

- surgical sterilization of the partner (vasectomy for 4 months minimum the first
dose/dosing) and with either a physical (e.g. condom, diaphragm, or other) or a
chemical (e.g., spermicide) barrier method from the time of screening and
throughout the study.

- two physical barrier method (e.g. condom, diaphragm, or other) with spermicide
for at least 14 days prior to the first dose and throughout the study.

In addition, female subjects of childbearing potential will be advised to remain
sexually inactive or to keep the same birth control method for at least 90 days
following the last dose.

6. For a female of non childbearing potential: must have undergone one of the following
sterilization procedures at least 6 months prior to the first dose:

- hysteroscopic sterilization;

- bilateral tubal ligation or bilateral salpingectomy;

- hysterectomy;

- bilateral oophorectomy. or be postmenopausal with amenorrhea for at least 1 year
prior to the first dose and follicle stimulating hormone (FSH) serum levels
consistent with postmenopausal status as per PI or designee judgment.

7. A non vasectomized, male subject must agree to use a condom with spermicide or
abstain from sexual intercourse during the study until 90 days beyond the last dose
of study drug. (No restrictions are required for a vasectomized male provided his
vasectomy has been performed 4 months or more prior to first dose of study drug. A
male who has been vasectomized less than 4 months prior to study first dose must
follow the same restrictions as a non vasectomized male).

8. If male, must agree not to donate sperm from the first dose until 90 days after the
last dose.

9. Understands the study procedures in the informed consent form (ICF), and be willing
and able to comply with the protocol.

Exclusion Criteria:

1. Subject is mentally or legally incapacitated or has significant emotional problems at
the time of the screening visit or expected during the conduct of the study.

2. History or presence of clinically significant medical or psychiatric condition or
disease in the opinion of the PI or designee.

3. History of any illness that, in the opinion of the PI or designee, might confound the
results of the study or poses an additional risk to the subject by their
participation in the study.

4. History or presence of alcoholism or drug abuse within the past 2 years prior to the
first dose.

5. History or presence of hypersensitivity or idiosyncratic reaction to the study
drug(s) or related compounds.

6. History or presence of ventricular dysfunction or risk factors for Torsades de
Pointes (e.g., heart failure, cardiomyopathy, family history of Long QT Syndrome).

7. Female subjects with a positive pregnancy test or lactating.

8. Positive urine drug or alcohol results at screening or each check in.

9. Positive results at screening for human immunodeficiency virus (HIV), hepatitis B
surface antigen (HBsAg) or hepatitis C virus (HCV).

10. QTcF interval is >450 msec (males) or >460 msec (females) or has ECG findings deemed
abnormal with clinical significance by the PI or designee at screening.

11. Unable to refrain from or anticipates the use of:

- Any drug, including prescription and non prescription medications, herbal
remedies, or vitamin supplements beginning 14 days prior to the first dose and
throughout the study. Acetaminophen (up to 2 g per 24 hour period) may be
permitted during the study.

- Any drugs known to be significant inducers of CYP enzymes and/or P gp, including
St. John's Wort, for 28 days prior to the first dose and throughout the study.
Appropriate sources will be consulted by the PI or designee to confirm lack of
PK/pharmacodynamic interaction with study drug(s).

12. Has been on a diet incompatible with the on study diet, in the opinion of the PI or
designee, within the 28 days prior to the first dose and throughout the study.

13. Donation of blood or significant blood loss within 56 days prior to the first dose.

14. Plasma donation within 7 days prior to the first dose.

15. Participation in another clinical study within 28 days prior to the first dose. The
28 day window will be derived from the date of the last blood collection or dosing,
whichever is later, in the previous study to Day 1 of Period 1 of the current study.