Selective HDAC6 Inhibitor ACY-241 in Combination With Ipilimumab and Nivolumab

This is a Phase 1b, open-label, dose-escalation cohort study. The study will consist of a
dose escalation assessment of the safety and tolerability of ACY-241 administered
concurrently in combination with ipilimumab and nivolumab to patients with advanced
melanoma. Treatment will be divided into induction and maintenance phases.

Determine the safety, tolerability, dose-limiting toxicities (DLTs), maximum tolerated dose
(MTD), and recommended Phase 2 dose (RP2D) of ACY-241 in combination with ipilimumab at 1
mg/kg and nivolumab at 3 mg/kg every 3 weeks for 4 doses each during a 12-week induction
period, then administered with nivolumab at a flat dose of 240 mg every 2 weeks in
maintenance for up to 1 year in patients with unresectable Stage III/Stage IV melanoma.

It is anticipated that this clinical study will enable selection of the RP2D and dose
schedule of this 3-drug combination for further clinical testing. The trial will include an
assessment of the pharmacodynamic activity of ACY-241.

Inclusion Criteria:

1. Patients must have signed and dated an Institutional Review Board/Independent Ethics
Committee -approved written informed consent form in accordance with regulatory and
institutional guidelines. This must be obtained before the performance of any
protocol-related procedures that are not part of normal patient care

2. Patients must be willing and able to comply with scheduled visits, treatment
schedule, laboratory tests, tumor biopsies, and other requirements of the study.

3. All patients must be either Stage IIIb/c or Stage IV according to the American Joint
Committee on Cancer (AJCC) (7th edition) and have histologically-confirmed melanoma
that is felt to be surgically unresectable in order to be eligible. Please refer to
the AJCC Cancer Staging Manual, 7th edition for a description of tumor, lymph node,
metastasis and staging.

- All melanomas, except ocular/uveal melanoma, regardless of primary site of
disease will be allowed; mucosal melanomas are eligible.

- Patients must not have received prior anticancer treatment for metastatic
disease (for example, but not limited to, systemic, local, radiation,
radiopharmaceutical).

-Exceptions: Surgery for melanoma and/or postresection brain radiotherapy (RT)
if central nervous system (CNS) metastases and/or prior treatment with adjuvant
interferon (IFN) (as described in Exclusion Criterion 2).

- All patients must have their disease status documented by a complete physical
examination and imaging studies within 4 weeks prior to the first dose of study
drug. Imaging studies must include computerized tomography (CT) scan of neck,
chest, abdomen, pelvis, and all known sites of resected disease in the setting
of Stage IIIb/c or Stage IV disease, and brain magnetic resonance imaging
([MRI], brain CT allowable if MRI is contraindicated).

- The complete set of baseline radiographic images must be available before
treatment initiation.

4. Eastern Cooperative Oncology Group (ECOG) performance status score of 0 or 1.

5. Tumor tissue from the resected site of disease must be provided for biomarker
analyses

6. Prior treated CNS metastases must be without MRI evidence of recurrence for at least
4 weeks after treatment. Patients must be off immunosuppressive doses of systemic
steroids (≥ 10 mg/day prednisone or equivalent) for at least 14 days prior to study
drug administration, and must have returned to neurologic baseline status
postoperatively.

• The 4-week period of stability is measured after the completion of the neurologic
interventions (ie, surgery and/or radiation).

7. In addition to neurosurgery to treat CNS metastases, adjuvant radiation after the
resection of CNS metastasis is allowed. Immunosuppressive doses of systemic steroids
(doses ≥ 10 mg/day prednisone or equivalent) must be discontinued at least 14 days
before study drug administration.

8. Prior surgery that required general anesthesia must be completed at least 4 weeks
before study drug administration. Surgery requiring local/epidural anesthesia must be
completed at least 72 hours before study drug administration.

9. All baseline laboratory requirements will be assessed and should be obtained within
14 days of first dose of study drug. Screening laboratory values must meet the
following criteria:

- White blood cells ≥ 2000/µL

- Neutrophils ≥ 1500/µL

- Platelets ≥ 100 × 10³/µL

- Hemoglobin ≥ 9.0 g/dL

- Serum creatinine ≤ 1.5 × upper limit of normal (ULN) or creatinine clearance >
40 mL/minute (using Cockcroft/Gault formula)

10. Patient Re-enrollment: This study permits the re-enrollment of a patient that has
discontinued the study as a screen failure (ie, patient has not been dosed/has not
been treated). If re-enrolled, the patient must be re-consented.

11. Males and females ≥ 18 years of age.

12. Women of childbearing potential (WOCBP) must have a negative serum or urine pregnancy
test (minimum sensitivity 25 IU/L or equivalent units of human chorionic gonadotropin
hormone) within 24 hours prior to the start of study drug.

13. Women must not be breastfeeding.

14. Women of childbearing potential must agree to follow instructions for method(s) of
contraception for the duration of treatment with study drug(s) plus 5 half-lives of
study drug plus 30 days (duration of ovulatory cycle). The half-lives of nivolumab
and ipilimumab is up to 25 days and 18 days, respectively. Given the blinded nature
of this study, WOCBP should therefore use an adequate method to avoid pregnancy for a
total of 23 weeks posttreatment completion.

15. Males who are sexually active with WOCBP must agree to follow instructions for
method(s) of contraception for the duration of treatment with study drug(s) plus 5
half lives of the study drug(s) plus 90 days (duration of sperm turnover). The
half-lives of nivolumab and ipilimumab are up to 25 days and 18 days, respectively.
Given the blinded nature of this study, men should therefore use an adequate method
of contraception for a total of 31 weeks posttreatment completion.

16. Azoospermic males and WOCBP who are continuously not heterosexually active are exempt
from contraceptive requirements. However, they must still undergo pregnancy testing
as described in this section.

Investigators shall counsel WOCBP and male patients who are sexually active with WOCBP on
the importance of pregnancy prevention and the implications of an unexpected pregnancy.
Investigators shall advise WOCBP and male patients who are sexually active with WOCBP on
the use of highly effective methods of contraception. Highly effective methods of
contraception have a failure rate of < 1% when used consistently and correctly.

Exclusion Criteria:

1. Patients with carcinomatosis meningitis or a history of ocular/uveal melanoma are
excluded.

2. Patients with previous nonmelanoma malignancies are excluded unless a complete
resection or remission was achieved at least 2 years prior to study entry and no
additional therapy is required or anticipated to be required during the study period
(exceptions include, but are not limited to, nonmelanoma skin cancers, in situ
bladder cancer, in situ gastric cancer or gastrointestinal stromal tumor, in situ
colon cancers, in situ cervical cancers/dysplasia, or breast carcinoma in situ).

3. Patients with active, known, or suspected autoimmune disease. Patients with type I
diabetes mellitus, residual hypothyroidism due to autoimmune thyroiditis only
requiring hormone replacement, or skin disorders (such as vitiligo, psoriasis, or
alopecia) not requiring systemic treatment are permitted to enroll. For any cases of
uncertainty, it is recommended that the Principal Investigator be consulted prior to
signing informed consent.

4. Patients with a condition requiring systemic treatment with either corticosteroids (≥
10 mg daily prednisone or equivalent) or other immunosuppressive medications within
14 days of study drug administration. Inhaled or topical steroids are permitted in
the absence of active autoimmune disease.

5. Prior therapy for melanoma with the following exceptions which are allowed: 1)
surgery for the melanoma lesion(s), 2) adjuvant RT after neurosurgical resection for
CNS lesions, and 3) prior adjuvant IFN (see qualifier below). Specifically, patients
who received prior therapy with anti-PD-1, anti PD L1, anti-PD-L2, anti-CD137, or
anti-CTLA-4 antibody (including ipilimumab or any other antibody or drug specifically
targeting T cell costimulation or checkpoint pathways) are not eligible.

• Prior treatment with adjuvant IFN is allowed if completed ≥ 3 months prior to
treatment.

6. Treatment directed against the melanoma (eg, chemotherapy, targeted agents,
biotherapy, limb perfusion) that is administered after a prior complete resection
other than adjuvant radiation after neurosurgical resection and IFN for resected
melanoma.

7. Previous therapy with histone deacetylase inhibitor.

8. Any of the following laboratory abnormalities:

- ANC < 1,500/µL

- Platelet count < 100,000/µL

- Hematologic growth factors are not allowed at screening or during the first
cycle of treatment

- Hemoglobin < 9 g/dL (< 5.5 mmol/L; previous red blood cell transfusion is
permitted)

- Creatinine > 1.5 × ULN

- AST or ALT > 2.5 × ULN. For patients with liver metastasis, AST or ALT > 5 × ULN

- Serum total bilirubin > 1.5 mg/dL or > 3 × ULN for patients with hereditary
benign hyperbilirubinemia

9. Corrected QT interval (QTc) using Fridericia's formula value > 480 msec at screening;
family or personal history of long QTc syndrome or ventricular arrhythmias including
ventricular bigeminy at screening; previous history of drug induced QTc prolongation
or the need for treatment with medications known or suspected of producing prolonged
QTc intervals on electrocardiogram (ECG).

10. Congestive heart failure (New York Heart Association Class III or IV), myocardial
infarction within 12 months before starting study treatment, or unstable or poorly
controlled angina pectoris, including Prinzmetal variant angina pectoris.

11. Any serious or uncontrolled medical disorder or active infection that, in the opinion
of the Investigator, may increase the risk associated with study participation, study
drug administration, or would impair the ability of the patient to receive protocol
therapy.

12. Any positive test result for hepatitis B virus or hepatitis C virus indicating acute
or chronic infection.

13. Known history of testing positive for human immunodeficiency virus or known acquired
immunodeficiency syndrome.

14. History of Grade ≥ 3 allergy to human monoclonal antibodies.

15. Prisoners or patients who are involuntarily incarcerated.

16. Patients who are compulsorily detained for treatment of either a psychiatric or
physical (eg, infectious disease) illness.

17. Pregnant or nursing women.

18. Psychological, familial, sociological, or geographical conditions that potentially
hamper compliance with the study protocol and follow-up schedule; those conditions
should be discussed with the patient before registration in the trial.