Multilevel Models of Therapeutic Response in the Lungs
| Status: | Recruiting |
|---|---|
| Conditions: | Pulmonary |
| Therapuetic Areas: | Pulmonary / Respiratory Diseases |
| Healthy: | No |
| Age Range: | 12 - Any |
| Updated: | 11/7/2018 |
| Start Date: | January 2017 |
| End Date: | August 2020 |
| Contact: | Elizabeth Hartigan, RN MPH |
| Email: | elizabeth.hartigan@chp.edu |
| Phone: | 412-692-7060 |
When developing new medications for lung diseases like Cystic Fibrosis (CF), scientists
perform lab experiments using cells from the airways, physiology studies of how the lungs
change when a drug is given, and clinical studies to determine how drugs affect overall
health. The investigators of this study are seeking to develop computer models that will
predict how patients will respond to drugs by just doing lab studies on cell samples from
their noses. Such models would allow for medications to be developed more rapidly for all
patients and allow treatments to be personalized as well. In order to develop these computer
models a series of tests will be performed on patients who have CF. Tests will include
sampling cells from the nose and measuring lung physiology using a combination of different
imaging, breathing, and other studies performed both before and after participants take a
therapy. Similar tests will be performed on people who do not have CF, and on the parents of
the CF participants who carry a single CF gene because this will provide information on how
specific genes might affect CF lung disease.
perform lab experiments using cells from the airways, physiology studies of how the lungs
change when a drug is given, and clinical studies to determine how drugs affect overall
health. The investigators of this study are seeking to develop computer models that will
predict how patients will respond to drugs by just doing lab studies on cell samples from
their noses. Such models would allow for medications to be developed more rapidly for all
patients and allow treatments to be personalized as well. In order to develop these computer
models a series of tests will be performed on patients who have CF. Tests will include
sampling cells from the nose and measuring lung physiology using a combination of different
imaging, breathing, and other studies performed both before and after participants take a
therapy. Similar tests will be performed on people who do not have CF, and on the parents of
the CF participants who carry a single CF gene because this will provide information on how
specific genes might affect CF lung disease.
The goal of this research is to develop a series of interconnected models of therapeutic
response in the diseased lung, focused primarily on Cystic Fibrosis (CF), that will
ultimately provide a means for predicting in vivo response based on patient-specific in vitro
testing, allowing for the optimization and personalization of therapies. Investigators use
both human bronchial epithelial (HBE) and more recently human nasal epithelial (HNE) cell
cultures to study CF pathophysiology. The investigators performing this study have also
developed functional imaging biomarkers in the lung that provide organ level quantification
of CF lung physiology (mucociliary clearance and airway liquid absorption), and, more
recently, in silico systems models of lung physiology at both the cell and organ level. The
in silico models provide a framework of differential equations that describe how basic
physiological processes interact and contribute to experimental outcomes. Their use allows
these mechanisms to be more specifically differentiated. Here the investigators propose to
link in vitro and in vivo response by sampling and culturing HNE cell cultures from both
non-CF and CF subjects who will also perform a series of physiological assessments, including
functional imaging scans. The in silico models will facilitate linking therapeutic studies in
cells to therapeutic outcomes in patients.
1. CF PATIENTS will perform 2 study days.
Study day 1 will include:
1. nasal potential difference measurements
2. pulmonary function testing
3. inert gas washout testing
4. urine pregnancy testing
5. nasal cell sampling
6. nuclear MCC/ABS scan (to include inhalation of isotonic or hypertonic saline -
randomized order)
7. blood draw for CFTR genotyping if not already available.
Study day 2 will include
1. pulmonary function testing
2. urine pregnancy testing
3. nuclear MCC/ABS scan (to include inhalation of isotonic or hypertonic saline -
randomized order)
2. PARENTS OF ENROLLED CF patients who choose to participate will perform 1 study day which
will include:
1. nasal potential difference measurements
2. pulmonary function testing
3. inert gas washout testing
4. urine pregnancy testing
5. nasal cell sampling
6. nuclear MCC/ABS scan (to include inhalation of isotonic saline)
7. a single blood sample drawn for CFTR genotyping.
3. HEALTHY CONTROLS will perform 1 screening and 1 study day which will include:
1. pulmonary function testing
2. inert gas washout testing
3. urine pregnancy testing
4. nasal cell sampling
5. nuclear MCC/ABS scan (to include inhalation of isotonic saline)
6. a single blood sample drawn for CFTR genotyping (at screening).
response in the diseased lung, focused primarily on Cystic Fibrosis (CF), that will
ultimately provide a means for predicting in vivo response based on patient-specific in vitro
testing, allowing for the optimization and personalization of therapies. Investigators use
both human bronchial epithelial (HBE) and more recently human nasal epithelial (HNE) cell
cultures to study CF pathophysiology. The investigators performing this study have also
developed functional imaging biomarkers in the lung that provide organ level quantification
of CF lung physiology (mucociliary clearance and airway liquid absorption), and, more
recently, in silico systems models of lung physiology at both the cell and organ level. The
in silico models provide a framework of differential equations that describe how basic
physiological processes interact and contribute to experimental outcomes. Their use allows
these mechanisms to be more specifically differentiated. Here the investigators propose to
link in vitro and in vivo response by sampling and culturing HNE cell cultures from both
non-CF and CF subjects who will also perform a series of physiological assessments, including
functional imaging scans. The in silico models will facilitate linking therapeutic studies in
cells to therapeutic outcomes in patients.
1. CF PATIENTS will perform 2 study days.
Study day 1 will include:
1. nasal potential difference measurements
2. pulmonary function testing
3. inert gas washout testing
4. urine pregnancy testing
5. nasal cell sampling
6. nuclear MCC/ABS scan (to include inhalation of isotonic or hypertonic saline -
randomized order)
7. blood draw for CFTR genotyping if not already available.
Study day 2 will include
1. pulmonary function testing
2. urine pregnancy testing
3. nuclear MCC/ABS scan (to include inhalation of isotonic or hypertonic saline -
randomized order)
2. PARENTS OF ENROLLED CF patients who choose to participate will perform 1 study day which
will include:
1. nasal potential difference measurements
2. pulmonary function testing
3. inert gas washout testing
4. urine pregnancy testing
5. nasal cell sampling
6. nuclear MCC/ABS scan (to include inhalation of isotonic saline)
7. a single blood sample drawn for CFTR genotyping.
3. HEALTHY CONTROLS will perform 1 screening and 1 study day which will include:
1. pulmonary function testing
2. inert gas washout testing
3. urine pregnancy testing
4. nasal cell sampling
5. nuclear MCC/ABS scan (to include inhalation of isotonic saline)
6. a single blood sample drawn for CFTR genotyping (at screening).
Cystic Fibrosis Subjects: Inclusion Criteria
- Ages 12 or older
- Diagnosis of cystic fibrosis as determined by sweat test or genotype
- Clinically stable as determined by a physician co-investigator
Cystic Fibrosis Subjects: Exclusion Criteria
- Smokers or users of electronic cigarettes
- FEV1%p <30% of predicted
- Nursing, pregnant or unwilling to test for pregnancy
- Intolerant to hypertonic saline
- Unable or unwilling to discontinue hypertonic saline, Pulmozyme, and long acting
bronchodilators for 24 hrs before testing and short acting bronchodilators on testing
days.
CF parents: Inclusion Criteria
- Ages 18 and older
- Biological parent of a CF patient who is also enrolled in the study
CF parents: Exclusion Criteria
- Smokers or users of electronic cigarettes
- FEV1%p <30% of predicted
- Nursing, pregnant or unwilling to test for pregnancy
- Unwilling to discontinue long acting bronchodilators for 24 hrs before testing and
short acting bronchodilators on testing days.
- Unwilling to perform CFTR genotyping.
Healthy controls: Inclusion Criteria
- Ages 18 and older
- No history of lung disease
Healthy Controls: Exclusion Criteria
- Smokers or users of electronic cigarettes
- FEV1%p <70% of predicted
- Nursing, pregnant or unwilling to test for pregnancy
- Carriers of known disease causing CFTR mutations
- Unwilling to perform CFTR genotyping.
We found this trial at
1
site
200 Lothrop St
Pittsburgh, Pennsylvania 15213
Pittsburgh, Pennsylvania 15213
Principal Investigator: Tim Corcoran, Ph.D.
Phone: 412-692-7060
University of Pittsburgh Medical Center UPMC is one of the leading nonprofit health systems in...
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