H7N9 Vaccination With and Without AS03 and Unadjuvanted H3N2v Vaccination: Standard and Systems Biology Analyses

This is a single center, randomized, partially-blinded, Phase II, small, targeted,
prospective study in approximately 30 healthy male and non-pregnant female subjects aged 18
to 49 years old, inclusive, designed to evaluate and compare the immunogenicity between an
intramuscular monovalent inactivated influenza A/H7N9 virus vaccine manufactured by Sanofi
Pasteur given with and without AS03 adjuvant manufactured by GlaxoSmithKline, and an
intramuscular unadjuvanted monovalent inactivated influenza A/H3N2v virus vaccine
manufactured by Sanofi Pasteur. The primary objectives include assessing the serum anti-HA
hemagglutination-inhibition (HAI) response to influenza A/H7N9 antigen (with and without
adjuvant) at Day 57 (approximately one month after the second study vaccination with A/H7N9
vaccine ± AS03) and influenza A/H3N2v antigen at Day 29, identifying differentially expressed
genes in human immune cells on Days 2, 4, and 29, and identifying differentially abundant
cellular proteins in human immune cells on Days 2, 4, and 29. The secondary objectives are to
compare plasma cytokine and chemokine profiles at specific time points and between treatment
arms at post vaccination points, assess the neutralizing antibody responses to influenza
A/H7N9 antigen, identify differentially expressed genes in human immune cells on Days 2, 4,
and 8 following one intramuscular dose of influenza A/H3N2v , and identifying differentially
abundant cellular proteins in human immune cells on Days 2, 4, and 8 following one
intramuscular dose of influenza A/H3N2v vaccine compared to baseline assessments performed
prior to study vaccination.

Inclusion Criteria:

1. Provide written informed consent prior to initiation of any study procedures. 2. Are
able to understand and comply with planned study procedures and be available for all study
visits.

3. Are males or non-pregnant females, 18 to 49 years old, inclusive. 4. Are in good health
a. As determined by medical history and targeted physical examination, if indicated based
on medical history, to evaluate acute or currently ongoing chronic medical diagnoses or
conditions, defined as those that have been present for at least 90 days, that would affect
the assessment of the safety of subjects or the immunogenicity of study vaccinations.
Chronic medical diagnoses or conditions should be stable for the last 60 days. This
includes no change in chronic prescription medication, dose, or frequency as a result of
deterioration of the chronic medical diagnosis or condition in the 60 days prior to
enrollment. Any prescription change that is due to change of health care provider,
insurance company, etc., or that is done for financial reasons, as long as in the same
class of medication, will not be considered a deviation of this inclusion criterion. Any
change in prescription medication due to improvement of a disease outcome, as determined by
the site principal investigator or appropriate sub-investigator, will not be considered a
deviation of this inclusion criterion. Subjects may be on chronic or as needed (prn)
medications if, in the opinion of the site principal investigator or appropriate
sub-investigator, they pose no additional risk to subject safety or assessment of
reactogenicity and immunogenicity and do not indicate a worsening of medical diagnosis or
condition. Similarly, medication changes subsequent to enrollment and study vaccination are
acceptable provided there was no deterioration in the subject's chronic medical condition
that necessitated a medication change, and there is no additional risk to the subject or
interference with the evaluation of responses to study vaccination. Note: Topical, nasal,
and inhaled medications (with the exception of inhaled corticosteroids as outlined in the
Subject Exclusion Criteria (see Section 5.1.2)), herbals, vitamins, and supplements are
permitted.

5. Oral temperature is less than 100.4F. 6. Pulse is 50 to 115 bpm, inclusive. 7. Systolic
blood pressure is 85 to 150 mm Hg, inclusive. 8. Diastolic blood pressure is 55 to 95 mm
Hg, inclusive. 9. Erythrocyte sedimentation rate (ESR) is less than 30 mm per hour. 10.
Women of childbearing potential 2 must use an acceptable contraception method 3 from 30
days before first study vaccination until 60 days after last study vaccination a. Not
sterilized via tubal ligation, bilateral oophorectomy, hysterectomy or successful Essure®
placement (permanent, non-surgical, non-hormonal sterilization) with documented
radiological confirmation test at least 90 days after the procedure, and still menstruating
or <1 year of the last menses if menopausal. b. Includes, but is not limited to, non-male
sexual relationships abstinence from sexual intercourse with a male partner, monogamous
relationship with vasectomized partner who has been vasectomized for 180 days or more prior
to the subject receiving the first study vaccination, barrier methods such as condoms or
diaphragms with spermicide or foam, effective intrauterine devices, NuvaRing®, and licensed
hormonal methods such as implants, injectables or oral contraceptives ("the pill").

11.Women of childbearing potential must have a negative urine or serum pregnancy test
within 24 hours prior to study vaccination

Exclusion Criteria:

1. Have an acute illness 4, as determined by the site principal investigator or
appropriate sub-investigator, within 72 hours prior to study vaccination. a.Including
acute or chronic medical disease or condition, defined as persisting for at least 90
days that would place the subject at an unacceptable risk of injury, render the
subject unable to meet the requirements of the protocol, or may interfere with the
evaluation of responses or the subject's successful completion of this study.

2. Have any medical disease or condition that, in the opinion of the site principal
investigator or appropriate sub-investigator, is a contraindication to study
participation. a.Including acute or chronic medical disease or condition, defined as
persisting for at least 90 days that would place the subject at an unacceptable risk
of injury, render the subject unable to meet the requirements of the protocol, or may
interfere with the evaluation of responses or the subject's successful completion of
this study

3. Have immunosuppression as a result of an underlying illness or treatment, or use of
anticancer chemotherapy or radiation therapy (cytotoxic) within 3 years prior to study
vaccination.

4. Have known active neoplastic disease (excluding non-melanoma skin cancer) or a history
of any hematologic malignancy.

5. Have known human immunodeficiency virus (HIV), hepatitis B, or hepatitis C infection.

6. Have known hypersensitivity or allergy to eggs, egg or chicken protein, squalene-based
adjuvants, or other components of the study vaccines.

7. Have a history of severe reactions following previous immunization with licensed or
unlicensed influenza virus vaccines.

8. Have a personal or family history of narcolepsy.

9. Have a history of Guillain-Barré syndrome.

10. Have a history of convulsions or encephalomyelitis within 90 days prior to study
vaccination.

11. Have a history of a potentially immune-mediated medical condition.

12. Have a history of alcohol or drug abuse within 5 years prior to study vaccination.

13. Have any diagnosis, current or past, of schizophrenia, bipolar disease, or other
psychiatric diagnosis that may interfere with subject compliance or safety
evaluations.

14. Have been hospitalized for psychiatric illness, history of suicide attempt, or
confinement for danger to self or others within 10 years prior to study vaccination.

15. Have taken oral or parenteral (including intra-articular) corticosteroids of any dose
within 30 days prior to study vaccination. 16. Have taken high-dose inhaled
corticosteroids within 30 days prior to study vaccination. High-dose defined as >840
mcg/day of beclomethasone dipropionate CFC or equivalent. 17. Received licensed live
vaccine within 30 days prior to the first study vaccination, or plans to receive
licensed live vaccine within 30 days before or after each study vaccination.

18. Received licensed inactivated vaccine within 14 days prior to the first study
vaccination, or plans to receive licensed inactivated vaccine within 14 days before or
after each study vaccination.

19. Received immunoglobulin or other blood products (with exception of Rho D
immunoglobulin) within 90 days prior to study vaccination 20. Received an experimental
agent7 within 30 days prior to the first study vaccination, or expects to receive an
experimental agent8 during the 13-month study-reporting period. Including vaccine, drug,
biologic, device, blood product, or medication. Other than from participation in this
study.

21. Are participating or plan to participate in another clinical trial with an
interventional agent9 that will be received during the 13-month study-reporting period.
Including licensed or unlicensed vaccine, drug, biologic, device, blood product, or
medication.

22. Prior participation in a clinical trial of influenza A/H7 vaccine10 or have a history
of influenza A/H7 virus actual or potential exposure or infection prior to the first study
vaccination. and assigned to a group receiving influenza A/H7 vaccine, does not apply to
documented placebo recipients.

23. Prior participation in a clinical trial of influenza A/H3N2v vaccine11 or have a
history of influenza A/H3N2v virus actual or potential exposure or infection prior to the
first study vaccination.

24. Occupational exposure to or substantial direct physical contact12 with birds in the
past year or during the 28 days after each study vaccination. A.Casual contact with birds
at petting zoos or county or state fairs or having pet birds does not exclude subjects from
study participation.

25. Occupational exposure to or substantial direct physical contact13 with pigs in the past
year or during the 28 days after each study vaccination. a. Casual contact with pigs at
petting zoos or county or state fairs does not exclude subjects from study 26. Female
subjects who are breastfeeding or plan to breastfeed at any given time from the first study
vaccination until 30 days after the last study vaccination.

27. Plan to travel outside the US (continental US, Hawaii, and Alaska) within 28 days after
each study vaccination. 28. Blood donation or planned blood donation within 30 days before
enrollment until 30 days after the last blood draw for this study.