Study to Evaluate the Effects of BPN14770 on Scopolamine-induced Cognitive Impairment in Healthy Volunteers

A total of 38 subjects will be enrolled into the study. The study duration will be up to 12
weeks with 6 weeks of single-dose Treatment Visits. The study will consist of a Screening
Visit (up to 28 days prior to first study drug administration), six inpatient Treatment
Visits (Periods 1 through 6), and a Follow-Up/Early Termination Visit (7-10 days after the
last dose of study medication). An additional study visit may be necessary to complete the
required cognitive test familiarization if not completed during the Screening Visit. Each
Treatment Visit will occur approximately one week apart, allowing a 6 to 8 day washout
period.

Subjects will be randomized to 1 of 6 treatment sequences based on a computer-generated
randomization schedule. Subjects will receive all 6 treatments as specified by the treatment
sequence according to a 6 × 6 Williams Latin square design. The following treatments will be
administered:

A. Scopolamine placebo + BPN14770 placebo + donepezil placebo

B. Scopolamine 0.5 mg + BPN14770 placebo + donepezil placebo

C. Scopolamine 0.5 mg + BPN14770 10 mg + donepezil placebo

D. Scopolamine 0.5 mg + BPN14770 50 mg + donepezil placebo

E. Scopolamine 0.5 mg + donepezil 10 mg +BPN14770 placebo

F. Scopolamine 0.5 mg + BPN14770 50 mg + donepezil 10 mg

During each of the Treatment Visits (Periods 1 through 6), subjects will be admitted to the
Clinical Research Unit (CRU) the day prior to each study drug administration (Day -1) and
discharged the day after study drug administration (Day 2). On the morning of study drug
administration (Day 1), subjects will be given study drug (BPN14770, donepezil, or placebo)
with 240mL of room temperature water 2 hours prior to the scopolamine or scopolamine placebo
sc injection. The timing for study drug administration will be referred to as t-2 (Hour -2).
Breakfast should be available approximately 30 minutes following the morning drug
administration. Two hours after study drug administration, the scopolamine or scopolamine
placebo sc injection will be administered. The time at which the scopolamine or scopolamine
placebo sc injection is administered will be referred to as t0 (Hour 0).

Cognitive testing will be performed 30 minutes prior to treatment with scopolamine injection
and at hours 1, 2, 3, 4, and 6 post-scopolamine injection.

PK samples will be collected during the treatment period to confirm study drug is present.

Safety assessments throughout the study will include physical exams, ECGs, vital signs,
chemistry, hematology, and urinalysis.

Inclusion Criteria:

1. Healthy males or females between the ages of 18 to 55 years at Screening.

2. Body mass index between 18 kg/m2 to 33 kg/m2, inclusive, and body weight of >50 kg
(110 pounds).

3. Female subjects must be surgically sterile (bilateral tubal ligation, hysterectomy, or
bilateral oophorectomy at least 6 months prior to first study drug administration), at
least two years post-menopausal, or willing to either (1) utilize hormonal
contraception plus use one barrier method or (2) use two barrier methods of
contraception from initial screening until one month after taking the final dose.
Barrier methods of contraception include diaphragm, cervical cap, male condom, female
condom, and spermicidal foam and sponges. An intrauterine device (IUD) is also
considered a barrier method of contraception in this study; if the subject is using an
IUD, she will need to use an additional barrier method of contraception. Menopausal
status will be verified by testing for follicle stimulating hormone (FSH ≥25 mIU/mL)
at Screening. In addition, all females must have a negative blood test for pregnancy
within 28 days during the Screening period and negative urine test for pregnancy on
Day-1 of each Treatment Visit regardless of childbearing potential.

4. Male subjects must be willing to inform female partners of their participation in the
study and must agree to use adequate contraceptive methods (vasectomy performed at
least 6 months prior to first study drug administration, or use at least one barrier
method of birth control).

5. Able to understand the study procedures, voluntarily consent to participate in this
study, and provide written informed consent prior to start of any study-specific
procedures.

6. Willing and able to remain in the study unit for the required periods and return for
each treatment of the six treatment periods, including the outpatient visits.

Exclusion Criteria:

1. Clinically significant abnormality, in the Investigator's judgement, in Screening
hematology, chemistry, or urinalysis tests, or from medical history, social history,
vital sign, or physical examination

2. Active liver disease or positive serology results for hepatitis B surface antigen
(HbsAg), hepatitis C virus (HCV), or human immunodeficiency virus (HIV).

3. Abnormal liver function test at the Screening Visit (aspartate aminotransferase or
alanine aminotransferase >2 × the upper limit of normal [ULN]; total bilirubin >1.5 ×
ULN; or alkaline phosphatase >2 × ULN based on appropriate age and gender normal
values).

4. Current or past history of angle closure glaucoma, or diagnosis of angle closure
glaucoma.

5. Marked hypotension (systolic blood pressure [BP] ˂90 mmHg or diastolic BP ˂50 mmHg) or
hypertension (systolic BP ˃150 mmHg or diastolic BP ˃100 mmHg) based on sitting values
obtained. Out-of-range results may be repeated once at Screening. This exclusion
applies to the vital signs performed at Screening and on Day -1 on Treatment Period 1.

6. Marked bradycardia (heart rate ˂45 beats per minute [bpm]) or tachycardia (heart rate
˃110 bpm) based on supine ECG values obtained. Out-of-range results may be repeated
once at Screening. This exclusion applies to the vital signs performed at Screening
and on Day -1 on Treatment Period 1.

7. Current or past history of significant (in the Investigator's judgement)
cardiovascular, cerebrovascular, pulmonary, renal, or liver disease. Stable,
well-controlled hypertension and hyperlipidemias are allowed.

8. Clinically important or significant conduction abnormalities on single ECG or evidence
or history of long QT syndrome based on supine ECG values obtained at Screening.
Out-of-range results may be repeated once at Screening.

9. Current or past history of gastric or duodenal ulcers or other diseases of the
gastrointestinal tract that could interfere with absorption of study drug. Note:
Subjects with a history of appendectomy or cholecystectomy may be enrolled.

10. Active acute or chronic infectious diseases.

11. Unable to discontinue medications including anticholinergic agents, psychotropic
drugs, sedative antihistamines, or other centrally active medications [e.g., CNS-
penetrant beta blockers], and moderate to strong inhibitors or inducers of CYP3A4,
CYP2D6, or other cytochromes) 14 days prior to the first dose of study drug (Period 1,
Day 1) and during the study (Follow-Up). Other prescription or non-prescription drugs
such as antihypertensive or cholesterol lowering drugs are allowed, if, in the
Investigator's judgement, they would not interfere with the study medication or the
cognitive testing.

12. Unable to discontinue and abstain from over-the-counter, herbal preparations, dietary
supplements, nutraceuticals, vitamins and minerals at least 7 days prior to the first
dose of study drug and during the study. The one exception to this rule is
acetaminophen, which may be taken for minor ailments at doses up to 1000 mg per day.

13. Any history of alcohol or other substance abuse, including marijuana, within the
previous year prior to the Screening visit (per the current edition of the Diagnostic
and Statistical Manual of Mental Disorders, 5th Edition: DSM-5), or regular (daily)
consumption of alcohol exceeding two bottles of beer, or the equivalent amount of
other forms of alcohol (1 serving = 12 oz beer, 5.0 oz wine, or 1.5 oz distilled
spirits).

14. Any use of alcohol, grapefruit, marijuana, or other psychotropic agent within 12 hours
of admission into the CRU.

15. Active smokers or tobacco users (e.g., chew and snuff) who are unable to discontinue
tobacco use or nicotine-containing products (including e-cigarettes) at least 4 weeks
prior to Screening and to refrain from using during the study.

16. Inability or unwillingness to comply with the protocol or likely inability to complete
the study.

17. Participation in other clinical studies involving investigational drug within the
previous 30 days prior to the Screening Visit.

18. Donation of blood within the prior 4 weeks, or blood products within the prior 2
weeks, prior to first study drug administration.

19. Positive result for drugs of abuse, alcohol, or cotinine at Screening, or a positive
drug or alcohol (breath) result upon admission to CRU.

20. History of clinically significant drug allergy that includes symptoms such as
shortness of breath, rash, or edema. This includes known hypersensitivity to donepezil
hydrochloride, scopolamine, or belladonna, alkaloids.

21. Inability or unwillingness to perform the Cogstate cognitive function tests.

22. A suicidal ideation intensity score of 2 or higher per screening Columbia Suicide
Severity Rating Scale (C-SSRS) assessment and/or any suicidal behavior within the past
28 days.